Skip header and navigation

Refine By

37 records – page 1 of 4.

[Alimentary and oxygen deprivation as the modulator of the bone tissue physiological remodelling rate in young rats]

https://arctichealth.org/en/permalink/ahliterature86619
Source
Fiziol Zh. 2008;54(1):85-93
Publication Type
Article
Date
2008
Author
Litovka I H
Source
Fiziol Zh. 2008;54(1):85-93
Date
2008
Language
Ukrainian
Publication Type
Article
Keywords
Alkaline Phosphatase - blood - metabolism
Animals
Anoxia - metabolism - physiopathology
Body Weight - physiology
Bone Remodeling - physiology
Bone and Bones - metabolism
Caloric Restriction
Electrophoresis, Agar Gel
Gene Expression - physiology
Glycosaminoglycans - blood
Insulin-Like Growth Factor I - genetics
Liver - metabolism
Male
Melatonin - blood
Polymerase Chain Reaction
Rats
Rats, Wistar
Abstract
Influencing of 28-days alimentary deprivation and intermittent hypoxia to 3 months Wistar male rats (n = 30) bone tissue physiological remodelling is studied. We investigated 3 groups of animals, I group was control, II--food limitation mode (-40% in relation to the normal ration) and III--animals, which breathed hypoxic gas mixture with 13% O2 in nitrogen (HGM) during 4 hours in the intermittent mode (10 min of deoxygenation and 10 min of reoxygenation). It is showed significant increasing of melatonin and glicozaminoglikans levels in the rats II and III gr. serum. Activity of alkaline phosphatase in the bone tissue of III gr. rats increased and in the bone tissue and serum of II gr. rats for 1.2 and 1.4 time accordingly. We registered significant increasing of serum acid phosphatase activity in animals, which have breathed hypoxic gas mixture. The IGF-I gene expression level did not change practically in both experimental groups. We conclude, that alimentary deprivation and intermittent hypoxia have positive effects on the physiological remodelling of bone tissue.
PubMed ID
18416190 View in PubMed
Less detail

[A method for differential cytolysis in the molecular genetic expert identification of material evidence: problems in optimizing the procedure].

https://arctichealth.org/en/permalink/ahliterature213534
Source
Sud Med Ekspert. 1996 Jan-Mar;39(1):16-21
Publication Type
Article
Author
L I Gyské
P L Ivanov
Source
Sud Med Ekspert. 1996 Jan-Mar;39(1):16-21
Language
Russian
Publication Type
Article
Keywords
Cell Fractionation - methods
DNA - analysis - isolation & purification
Electrophoresis, Agar Gel
Expert Testimony - methods - standards
Forensic Medicine - methods - standards - statistics & numerical data
Humans
Male
Polymerase Chain Reaction - methods - standards - statistics & numerical data
Regression Analysis
Russia
Spermatozoa - chemistry - ultrastructure
Abstract
The authors discuss the problem of selective derivation of the genetic material of spermatozoa for molecular genetic identification from mixed biological traces containing sperm on material evidence. Possible methods of improving the efficacy of differential lysis of cells present in mixed traces are analyzed. Effects of some routinely used extractants on biological substrata, most often contaminating the sperm in expert material, have been studied, and conditions for their most complete elimination from objects of investigation optimized.
PubMed ID
8669057 View in PubMed
Less detail

[A molecular genetic analysis of spinal muscular atrophy (SMA) in families at high risk from different regions of Ukraine]

https://arctichealth.org/en/permalink/ahliterature33908
Source
Tsitol Genet. 1997 Nov-Dec;31(6):75-81
Publication Type
Article
Author
A Iu Ekshiian
L A Livshits
A M Bychkova
N A Afanas'eva
I R Bariliak
Source
Tsitol Genet. 1997 Nov-Dec;31(6):75-81
Language
Russian
Publication Type
Article
Keywords
Adult
Child
Chimera - genetics
DNA - genetics - isolation & purification
DNA Primers
Electrophoresis, Agar Gel
English Abstract
Exons - genetics
Gene Deletion
Heterozygote
Homozygote
Humans
Molecular Biology
Polymerase Chain Reaction - methods
Research Support, Non-U.S. Gov't
Risk factors
Spinal Muscular Atrophies of Childhood - genetics
Ukraine
Abstract
The results of DNA analysis of deletion in exons 7 and 8 of SMN gene, and exon 5 of NAIP gene in 24 SMA-families from Ukraine are presented. Deletions of SMN exons 7 and 8, or 7 were found in 46 (97.9%) of 47 SMA-chromosomes. A homozygous deletion of NAIP exon 5 was demonstrated in 4 (19%) of 21 SMA-families. The authors have demonstrated that in 2 SMA patients with homozygous deletion SMN exon 7 only, the remaining SMN exon 8 was a part of a chimeric CBCD41/SMN gene.
PubMed ID
9591348 View in PubMed
Less detail

Apolipoprotein E polymorphism, serum lipids and occurrence of "double pre-betalipoproteinemia' (DPBL) in subjects from two different populations.

https://arctichealth.org/en/permalink/ahliterature235273
Source
Atherosclerosis. 1987 May;65(1-2):23-8
Publication Type
Article
Date
May-1987
Author
A. Pagnan
G. Zanetti
A. Bonanome
S. Biffanti
C. Ehnholm
L. Keso
M. Lukka
Source
Atherosclerosis. 1987 May;65(1-2):23-8
Date
May-1987
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Aged
Apolipoproteins E - blood - genetics
Child
Cross-Sectional Studies
Electrophoresis, Agar Gel
Female
Finland
Genetics, Population
Humans
Hyperlipoproteinemias - genetics
Italy
Lipids - blood - genetics
Lipoproteins, VLDL - blood - genetics
Male
Middle Aged
Phenotype
Polymorphism, Genetic
Abstract
The association between apolipoprotein E phenotype and the presence of 2 electrophoretic populations of very low density (VLDL) lipoproteins from human sera, double pre-beta(VLDL)lipoproteinemia (DPBL), was studied in 2 groups of subjects, one from Italy and one from Finland. In both populations the prevalence of DPBL was significantly higher in subjects with E4/4 and E4/3 phenotypes than in the other phenotypes not containing the E4 isoprotein. This finding suggests that the presence of a structural variant of the apo E protein, the isoprotein E4, may be causally related to the appearance of DPBL phenomenon.
PubMed ID
3496894 View in PubMed
Less detail

The C4 system: formal and population genetics.

https://arctichealth.org/en/permalink/ahliterature41467
Source
Hum Genet. 1979;50(2):187-92
Publication Type
Article
Date
1979
Author
B. Olaisen
P. Teisberg
R. Jonassen
T. Gedde-Dahl
Source
Hum Genet. 1979;50(2):187-92
Date
1979
Language
English
Publication Type
Article
Keywords
Complement C4 - genetics
Electrophoresis, Agar Gel
Female
Gene Frequency
Genotype
Humans
Male
Norway
Pedigree
Phenotype
Polymorphism, Genetic
Abstract
The present study shows that the C4 system as investigated by high voltage agarose gel electrophoresis is highly polymorphic. In a series of unrelated Norwegian adults, where C4 types have been ascertained through segregation in families, six different haplotypes have been found to occur with a frequency exceeding 1%. The genotype frequencies in the population fit expected Hardy-Weinberg distribution. In family material comprising 89 matings with 327 children the distribution of offspring is as expected according to autosomal codominant inheritance of haplotypes.
PubMed ID
511133 View in PubMed
Less detail

A case of Q fever acquired in Sweden and isolation of the probable ethiological agent, Coxiella burnetii from an indigenous source.

https://arctichealth.org/en/permalink/ahliterature195805
Source
Scand J Infect Dis. 2000;32(6):605-7
Publication Type
Article
Date
2000
Author
S. Rustscheff
L. Norlander
A. Macellaro
A. Sjöstedt
S. Vene
M. Carlsson
Author Affiliation
Department of Internal Medicine, Värnamo General Hospital, Sweden.
Source
Scand J Infect Dis. 2000;32(6):605-7
Date
2000
Language
English
Publication Type
Article
Keywords
Animals
Anti-Bacterial Agents - therapeutic use
Coxiella burnetii - isolation & purification
DNA, Bacterial - analysis
Diagnosis, Differential
Doxycycline - therapeutic use
Electrophoresis, Agar Gel
Housing, Animal
Humans
Male
Middle Aged
Poaceae - microbiology
Polymerase Chain Reaction
Q Fever - diagnosis - drug therapy - transmission
Sweden
Abstract
Serologically verified indigenous Q fever is described in a 52-y-old male, who presented with persistent fever, muscle and joint pain, headache and non-purulent cough. Institution of doxycycline resulted in prompt recovery. Coxiella burnetii was isolated from mouldy hay in a barn. The strain differs from previously isolated ones in Sweden.
PubMed ID
11200368 View in PubMed
Less detail

Cell-surface-bound nucleic acids: Free and cell-surface-bound nucleic acids in blood of healthy donors and breast cancer patients.

https://arctichealth.org/en/permalink/ahliterature17606
Source
Ann N Y Acad Sci. 2004 Jun;1022:221-7
Publication Type
Article
Date
Jun-2004
Author
Pavel P Laktionov
Svetlana N Tamkovich
Elena Yu Rykova
Olga E Bryzgunova
Andrey V Starikov
Nina P Kuznetsova
Valentin V Vlassov
Author Affiliation
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. lakt@niboch.nsc.ru
Source
Ann N Y Acad Sci. 2004 Jun;1022:221-7
Date
Jun-2004
Language
English
Publication Type
Article
Keywords
Breast Neoplasms - blood - diagnosis - genetics - pathology
Chelating Agents - pharmacology
Comparative Study
DNA - blood - isolation & purification - metabolism
Deoxyribonucleases - metabolism
Edetic Acid - pharmacology
Electrophoresis, Agar Gel
Erythrocytes - drug effects
Female
Fibroadenoma - blood - diagnosis - genetics - pathology
Humans
Leukocytes - drug effects
Neoplasm Staging
Nucleic Acids - blood - isolation & purification - metabolism
RNA - blood - isolation & purification - metabolism
Research Support, Non-U.S. Gov't
Ribonucleases - metabolism
Trypsin - pharmacology
Abstract
Concentrations of extracellular DNA and RNA in the blood of healthy donors and patients with malignant and nonmalignant breast tumors were investigated. Cell-surface-bound extracellular DNA and RNA were detached by PBS-EDTA treatment or mild trypsin treatment of erythrocytes and leukocytes. In healthy donors, almost all extracellular nucleic acids (98%) are bound at the surface of blood cells. In the blood of cancer patients, extracellular nucleic acids were found in plasma and not at the cell surface. In patients with nonmalignant breast tumors, extracellular nucleic acids were found both at the surface of blood cells and in plasma. In healthy donors, the cell-surface-bound DNA is represented by 20-kbp DNA fragments and smaller fragments that varied in amounts in different fractions.
PubMed ID
15251964 View in PubMed
Less detail

Characterisation of streptomycin resistance determinants in Danish isolates of Salmonella Typhimurium.

https://arctichealth.org/en/permalink/ahliterature10433
Source
Vet Microbiol. 2000 Jul 3;75(1):73-82
Publication Type
Article
Date
Jul-3-2000
Author
L. Madsen
F M Aarestrup
J E Olsen
Author Affiliation
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigboejlen 4, DK 1870 C, Frederiksberg, Denmark.
Source
Vet Microbiol. 2000 Jul 3;75(1):73-82
Date
Jul-3-2000
Language
English
Publication Type
Article
Keywords
Animals
Blotting, Southern - veterinary
Cattle
Cattle Diseases - drug therapy - microbiology
Colony Count, Microbial
Conjugation, Genetic - genetics
DNA Primers - chemistry
DNA, Bacterial - chemistry - isolation & purification
Denmark
Drug Resistance, Microbial - genetics
Electrophoresis, Agar Gel - veterinary
Humans
Nucleotidyltransferases - chemistry - genetics
Phosphotransferases (Alcohol Group Acceptor) - chemistry - genetics
Polymerase Chain Reaction - veterinary
Research Support, Non-U.S. Gov't
Salmonella Infections, Animal - drug therapy
Salmonella typhimurium - chemistry - drug effects - genetics
Sequence Analysis, DNA
Streptomycin - pharmacology
Swine
Swine Diseases - drug therapy - microbiology
Variation (Genetics) - genetics
Abstract
Fifty six Danish streptomycin (Sm) resistant isolates of Salmonella enterica serotype Typhimurium from pigs (n=34), calves (n=3) and humans (n=19) were characterised with respect to co-resistances (14 drugs), transferability of Sm-resistance by conjugation, genetic determinants encoding Sm-resistance and diversity with respect to localisation of genes in the genome and DNA-sequences. Forty-six strains carried resistance(s) other than Sm-resistance. Nineteen different co-resistance patterns were observed and tetracycline was the most commonly observed resistance in these patterns. In 22 of the strains, Sm-resistance was transferred by conjugation. Eleven strains contained the gene aadA only, six strains contained aadA+strA+strB, and 35 strains contained strA+strB. Partial sequences of aadA were obtained from four strains. Three strains showed identical sequences to a published aadA sequence from the transposon Tn7, and in one strain the sequence showed one synonymous substitution compared to this sequence. Partial sequences were obtained of strA and strB in seven strains. The sequence of strB was identical to the published sequence of the plasmid RSF1010 in all strains. All seven sequences of strA were identical and differed from the sequence of strA in RSF1010 by two non-synonymous substitutions.
PubMed ID
10865153 View in PubMed
Less detail

Characterization of a new LCAT mutation causing familial LCAT deficiency (FLD) and the role of APOE as a modifier gene of the FLD phenotype.

https://arctichealth.org/en/permalink/ahliterature150387
Source
Atherosclerosis. 2009 Dec;207(2):452-7
Publication Type
Article
Date
Dec-2009
Author
Alexis Baass
Hanny Wassef
Michel Tremblay
Lise Bernier
Robert Dufour
Jean Davignon
Author Affiliation
Hyperlipidemia and Atherosclerosis Research Group, Clinical Research Institute of Montreal (IRCM), Montreal, QC, Canada. a.baass@umontreal.ca
Source
Atherosclerosis. 2009 Dec;207(2):452-7
Date
Dec-2009
Language
English
Publication Type
Article
Keywords
Adult
Aged
Apolipoprotein A-I - blood
Apolipoprotein E2 - blood - genetics
Apolipoproteins B - blood
Biological Markers - blood
Cholesterol, HDL - blood
Cholesterol, LDL - blood
Chromatography, Gel
DNA Mutational Analysis
Electrophoresis, Agar Gel
Frameshift Mutation
Genetic Predisposition to Disease
Heterozygote
Homozygote
Humans
Hyperlipoproteinemia Type III - blood - enzymology - genetics
Lecithin Acyltransferase Deficiency - blood - enzymology - genetics
Male
Middle Aged
Pedigree
Phenotype
Phosphatidylcholine-Sterol O-Acyltransferase - genetics
Quebec
Risk factors
Sequence Deletion
Triglycerides - blood
Young Adult
Abstract
Familial LCAT deficiency (FLD) is a disease characterized by a defect in the enzyme lecithin:cholesterol acyltransferase (LCAT) resulting in low HDL-C, premature corneal opacities, anemia as well as proteinuria and renal failure. We have identified the first French Canadian kindred with familial LCAT deficiency. Two brothers, presenting classical signs of FLD, were shown to be homozygous for a novel LCAT mutation. This c.102delG mutation occurs at the codon for His35 and causes a frameshift that stops transcription at codon 61 abolishing LCAT enzymatic activity both in vivo and in vitro. It has a dramatic effect on the lipoprotein profile, with an important reduction of HDL-C in both heterozygotes (22%) and homozygotes (88%) and a significant decrease in LDL-C in heterozygotes (35%) as well as homozygotes (58%). Furthermore, the lipoprotein profile differs markedly between the two affected brothers who had different APOE genotypes. We propose that APOE could be an important modifier gene explaining heterogeneity in lipoprotein profiles observed among FLD patients. Our results suggest that a LCAT-/- genotype associated with an APOE epsilon2 allele could be a novel mechanism leading to dysbetalipoproteinemia.
PubMed ID
19515369 View in PubMed
Less detail

37 records – page 1 of 4.