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Acute phase proteins serum concentrations in children are related to urinary iodine excretion.

https://arctichealth.org/en/permalink/ahliterature29304
Source
Rocz Akad Med Bialymst. 2005;50:279-83
Publication Type
Article
Date
2005
Author
S A Lialikau
L L Haurylik
M. Sobieska
N M Klachko
W. Samborski
Author Affiliation
State Medical University, Grodno, Belarus.
Source
Rocz Akad Med Bialymst. 2005;50:279-83
Date
2005
Language
English
Publication Type
Article
Keywords
Acute-Phase Proteins - metabolism
Adolescent
Child
Chromatography, Affinity
Concanavalin A - metabolism
Female
Glycosylation
Humans
Immunoelectrophoresis
Iodine - urine
Ligands
Male
Radioimmunoassay
Thyroid Hormones - metabolism
Abstract
PURPOSE: The paper presents links between iodine provision and selected acute phase proteins' (APP) serum concentrations as well as their glycosylations profiles (investigated with the use of affinity immunoelectrophoresis with Concanavalin A as ligand) in children. MATERIAL AND METHOD: 116 children (58 girls and 58 boys) were enrolled. Iodine level was measured in the morning (7:30-8:30) urine portion, using Cr-As method. According to iodine level children were divided into two groups. The first one consisted of 56 children with decreased iodine level (lower than 100 micrograms/L), second--60 children with iodine level higher than 100 micrograms/L. In serum the concentration of ferritin, beta2-microglobulin (beta2-MG), thyroxin (T4), triiodothyronin (T3), thyrotrophic hormone (TSH) were measured by radioimmunoassay (BELORIS, Belarus). Concentrations of APP: C-reactive protein (CRP), alphal-acid glycoprotein (AGP), alphal-antichymotrypsin (ACT), alphal-antitrypsin (AT), haptoglobin (Hp), alpha2-macroglobulin (A2-M), ceruloplasmin (Cp) and transferrin (Tf) were measured in sera samples by rocket immunoelectrophoresis acc. to Laurell with antibodies and standard from DakoCytomation, Denmark. Microheterogeneity of AGP, ACT and Tf was estimated using affinity immunoelectrophoresis with ConA as a ligand, acc. to Bøg-Hansen. RESULTS: It was established, that CRP level was lower than upper limit of normal range. Levels of other investigated proteins were reliably dependent on the level of iodine. Especially for AGP lower level was observed for children of the group with low iodine level. In children with low iodine level along with the decrease of serum AGP concentration altered glycosylations profile was observed, namely decrease in the content of variant non-reactive to ConA (W0) and increase in content of weakly reactive (W1) and reactive (W2) variants content, which resulted in increase of the reactivity coefficient (AGP-RC). Similar tendency in alterations of distinctly glycosylated variants in relation to iodine level could be shown for ACT. Serum concentration of any investigated protein was not dependent on the concentration of the hormones of pituitary-thyroid system. CONCLUSIONS: It seems that the influence of the iodine level is direct, not via thyroid hormones. It could be suggested that in euthyroid children with low iodine excretion with urine a hidden iodine deficiency is already registered by the regulatory mechanisms and a kind of acute phase reaction is started, may be in order to increase iodine uptake and storage.
PubMed ID
16358983 View in PubMed
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Age-associated changes in mitogen-induced lymphoproliferation and lymphokine production in the long-lived brown-Norway rat: effect of caloric restriction.

https://arctichealth.org/en/permalink/ahliterature62026
Source
Mech Ageing Dev. 1995 Sep 7;83(2):103-16
Publication Type
Article
Date
Sep-7-1995
Author
I M Goonewardene
D M Murasko
Author Affiliation
Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia 19129, USA.
Source
Mech Ageing Dev. 1995 Sep 7;83(2):103-16
Date
Sep-7-1995
Language
English
Publication Type
Article
Keywords
Aging - metabolism
Animals
Concanavalin A - pharmacology
Energy intake
Interferons - biosynthesis
Lymphokines - biosynthesis
Rats
Rats, Inbred BN
Research Support, U.S. Gov't, P.H.S.
Abstract
We have previously demonstrated that age-related declines in Concanavalin A (ConA), induced proliferation and lymphokine production, occur in ad-libitum fed Brown Norway (AL BN) rats. Since caloric restriction (CR) extends lifespan, we expected that the age related changes in immune parameters would be delayed by CR. CR does act to delay age-related changes in proliferation in response to ConA. In addition, CR postpones the plateau in ConA induced interferon (IFN) production seen after 23 months of age in AL rats. However, CR does not postpone the age-related decline in ConA induced interleukin-2 (IL-2) production. Therefore, ConA induced IFN production maybe a good candidate as an early marker of physiologic aging, while ConA induced proliferative response is a possible candidate for a marker of late stages of aging.
PubMed ID
8569285 View in PubMed
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[Analysis of polymorphism of the interleukin-4 gene of healthy and HIV-infected persons]

https://arctichealth.org/en/permalink/ahliterature7401
Source
Zh Mikrobiol Epidemiol Immunobiol. 2001 Nov-Dec;(6):28-32
Publication Type
Article
Author
V I Konenkov
M V Smol'nikova
V A Kozlov
S V Sennikov
L P Siziakina
E. Taleb
Author Affiliation
Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, Novosibirsk, Russia.
Source
Zh Mikrobiol Epidemiol Immunobiol. 2001 Nov-Dec;(6):28-32
Language
Russian
Publication Type
Article
Keywords
Adolescent
Adult
Alleles
Cells, Cultured
Comparative Study
Concanavalin A - pharmacology
English Abstract
European Continental Ancestry Group
HIV Infections - genetics - immunology
Homozygote
Humans
Interleukin-4 - biosynthesis - genetics
Leukocytes, Mononuclear - drug effects - immunology
Male
Middle Aged
Polymorphism, Genetic
Promoter Regions (Genetics) - genetics
Sex Factors
Statistics, nonparametric
Abstract
The distribution of the allel variants of the promoter area (C = 590T) of the interleukin-4 (IL-4) gene in HIV-infected and relatively healthy representatives of the Caucasoid population has been studied. The relationship between the genotypes of this polymorphism and the production of IL-4 by mononuclear cells of peripheral blood as well as distribution of IL-4 genotypes among males and females is analyzed. The occurrence of the homozygous combination of the allel variant C/C of the promoter of IL-4 has been shown to prevail almost twofold over the occurrence of the variant C/T among healthy donors and HIV-infected patients. Sexual differences play an essential role in the character of inheriting the allel variants of the genes of IL-4, the presence of the homozygous variant C/C or T/T being a risk factor of HIV infection in males. As revealed in this study, in the peripheral blood of healthy donors mononuclear cells having genotype C/C differ from cells with the heterozygous variant C/T in higher spontaneous production of IL-4 and, simultaneously, in lower capacity for the activation of its production in response to stimulation with mitogen. In HIV-infected patients mononuclear cells differ in higher spontaneous production of IL-4 in comparison with controls. We may thus infer that the human genotype controlling the initial level of the production of IL-4 by lymphocytes Th2 may influence the intensity of antibody production in the process of infection.
PubMed ID
11881490 View in PubMed
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Cellular and reaginic immune responses to ragweed antigen E in inbred rats.

https://arctichealth.org/en/permalink/ahliterature57918
Source
Int Arch Allergy Appl Immunol. 1980;61(3):259-70
Publication Type
Article
Date
1980
Author
T J Yoo
C Y Kuo
Source
Int Arch Allergy Appl Immunol. 1980;61(3):259-70
Date
1980
Language
English
Publication Type
Article
Keywords
Animals
Antibody formation
Comparative Study
Concanavalin A - pharmacology
Female
Immunity, Cellular
Immunization, Passive
In Vitro
Lymphocyte Activation
Phytohemagglutinins - pharmacology
Plant Lectins
Pollen - immunology
Rats
Rats, Inbred Strains
Reagins - biosynthesis
Research Support, U.S. Gov't, Non-P.H.S.
Species Specificity
Time Factors
Abstract
The cellular and reaginic immune responses to ragweed were examined in six strains of inbred rats. Three differentiable patterns of primary reaginic responses were observed: (a) Lewis rats--good uniform responders--produced high titers of IgE antibody for a short (less than 2 weeks) time; (b) Brown Norway rats--good uniform responders--produced high titers of IgE antibody for a long time (more than 1 month), and (c) intermediate responders--Buffalo, ACI, Fischer 344 and Wistar-Furth rats--produced poor-to-moderate titers of IgE for an intermediate time. There were no secondary reaginic immune responses to ragweed AgE in these six strains of rats. Furthermore, extensive study of Lewis rats indicated that IgE antibody production in primary immunization could be suppressed by passive transfer of either AgE-primed spleen lymphocytes, AgE-primed blood lymphocytes, or Con-A stimulated lymphocytes. Cellular responses to AgE and PHA stimulation were suppressed in rats receiving AgE-primed lymphocytes or Con-A stimulated lymphocytes.
PubMed ID
7353902 View in PubMed
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Dietary effect of tocopherols and tocotrienols on the immune function of spleen and mesenteric lymph node lymphocytes in Brown Norway rats.

https://arctichealth.org/en/permalink/ahliterature61827
Source
Biosci Biotechnol Biochem. 1999 Oct;63(10):1697-702
Publication Type
Article
Date
Oct-1999
Author
J Y Gu
Y. Wakizono
Y. Sunada
P. Hung
M. Nonaka
M. Sugano
K. Yamada
Author Affiliation
Department of Bioscience and Biotechnology, Kyushu University, Fukuoka, Japan.
Source
Biosci Biotechnol Biochem. 1999 Oct;63(10):1697-702
Date
Oct-1999
Language
English
Publication Type
Article
Keywords
Animals
Concanavalin A - pharmacology
Diet
Dinoprostone - blood
Immunoglobulins - biosynthesis
Interferon Type II - biosynthesis
Lymph Nodes - drug effects - immunology
Lymphocyte Subsets - immunology - metabolism
Lymphocytes - drug effects - immunology
Male
Peroxides - blood
Rats
Research Support, Non-U.S. Gov't
Spleen - drug effects - immunology
Time Factors
Tumor Necrosis Factor-alpha - biosynthesis
Vitamin E - administration & dosage - analogs & derivatives - pharmacology
Abstract
The immunoregulatory effects of dietary alpha-tocopherol (Toc) and tocotrienols (T-3) on humoral and cell-mediated immunity and cytokine productions were examined in Brown Norway rats. We found that the IgA and IgG productivity of spleen and mesenteric lymph node (MLN) lymphocytes was significantly enhanced in the rats fed on Toc or T-3, irrespective of concanavalin A (Con A) stimulation of the lymphocytes. On the contrary, the IgE productivity of lymphocytes from the rats fed on Toc or T-3 was less without Con A stimulation, but was greater in the presence of Con A, especially in the T-3 group. Toc or T-3 feeding significantly decreased the proportion of CD4+ T cells and the ratio of CD4+/CD8+ in both spleen and MLN lymphocytes of the rats fed on Toc or T-3. The interferon-gamma productivity of MLN lymphocytes was higher in the rats fed on Toc or T-3 than in those fed on a control diet in the presence of Con A, while that of spleen lymphocytes was lower in the rats fed on Toc or T-3. In addition, T-3 feeding decreased the productivity of tumor necrosis factor-alpha of spleen lymphocytes, while it enhanced the productivity of MLN lymphocytes. These results suggest that oral administration of Toc and T-3 affects the proliferation and function of spleen and MLN lymphocytes.
PubMed ID
10586497 View in PubMed
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[Functional activity of peritonal macrophages in liver immune damage of cellular and antibody genesis in mice]

https://arctichealth.org/en/permalink/ahliterature89020
Source
Fiziol Zh. 2009;55(1):36-42
Publication Type
Article
Date
2009
Author
Martynova T V
Aleksieieva I M
Source
Fiziol Zh. 2009;55(1):36-42
Date
2009
Language
Ukrainian
Publication Type
Article
Keywords
Animals
Antibodies, Monoclonal - immunology - pharmacology
Concanavalin A - pharmacology
Disease Models, Animal
Hepatitis, Autoimmune - immunology
Immunoglobulin Fragments - immunology
Liver - drug effects - immunology
Macrophages, Peritoneal - immunology - physiology
Male
Mice
Mice, Inbred CBA
Phagocytosis - drug effects - immunology - physiology
T-Lymphocytes - drug effects - immunology
gamma-Globulins - immunology
Abstract
The aim of present work was to compare the functional activity of peritoneal macrophages (Mf) at T-cellular and antibody induced hepatitis in mice of CBA line. T-cellular hepatitis was caused by concanavalin A (ConA), antibody-induced hepatitis was caused by administration of xenogenic anti-liver antibodies: gamma-globulin fractions of antihepatocytotoxic serum (gamma-AHCS). It was found that single injection of ConA or gamma-AHCS caused damage of liver with cytolytic syndrome through 20 hours. Functional activity of Mf in these conditions was significantly different. Application of ConA resulted in the decrease in phagocytosis of latex particles and oxygen-dependent metabolism; application of gamma-AHCS--to increase of these processes. Weakening of Mf activity may be one of the reasons for the decrease of dead cell eliminations that results in the maintenance of inflammatory reaction. At the same time significant amplification of phagocytic Mf activity may be one of the pathways of free radical endogenic sources increase that causes cell alteration and plays its role as mediators at inflammation.
PubMed ID
19441713 View in PubMed
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[Functional activity of the peritoneal macrophages in mice with concanavalin A-induced hepatitis]

https://arctichealth.org/en/permalink/ahliterature85073
Source
Fiziol Zh. 2007;53(5):47-52
Publication Type
Article
Date
2007
Author
Martynova T V
Aleksiuk L I
Source
Fiziol Zh. 2007;53(5):47-52
Date
2007
Language
Ukrainian
Publication Type
Article
Keywords
Alanine Transaminase - blood
Alkaline Phosphatase - blood
Animals
Aspartate Aminotransferases - blood
Concanavalin A
Disease Models, Animal
Hepatitis, Autoimmune - enzymology - etiology - immunology
Macrophages, Peritoneal - immunology - physiology
Male
Mice
Mice, Inbred CBA
Microspheres
Phagocytosis - immunology
Abstract
Hepatitis of T-cell genesis, a model of autoimmune damage of human liver, was caused in mice of CBA line by concanavalin A (Con A). The damage of liver was examined by activities of transaminases (alanine aminotransferase, aspartate aminotransferase) and alkaline phosphatase in mice blood plasma. Functional activity of macrophages in peritoneal fluid was studied by examination of phagocytosis of latex particles and oxygen--dependent metabolism (nitro-blue-tetrazolium- test). We demonstrated that a single intravenous injection of Con A in different doses (15 and 30 Mg/kg of body weight) caused acute hepatic damage in 20 hours. Weakening of macrophage functional activities may be one of the reasons of decrease in dead cells elimination following induction of immune hepatitis, it may support inflammatory reaction and promote the development of autoimmune process.
PubMed ID
18080493 View in PubMed
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Human immunoglobulin preparations for intravenous use prevent experimental autoimmune uveoretinitis.

https://arctichealth.org/en/permalink/ahliterature57713
Source
Int Immunol. 1993 Dec;5(12):1559-67
Publication Type
Article
Date
Dec-1993
Author
A. Saoudi
V. Hurez
Y. de Kozak
J. Kuhn
S V Kaveri
M D Kazatchkine
P. Druet
B. Bellon
Author Affiliation
INSERM U28, Hôpital Broussais, Paris, France.
Source
Int Immunol. 1993 Dec;5(12):1559-67
Date
Dec-1993
Language
English
Publication Type
Article
Keywords
Animals
Antigens - immunology
Arrestin
Autoimmune Diseases - immunology - prevention & control
Concanavalin A - immunology
Eye Proteins - immunology
Female
Flow Cytometry
Humans
Immunoglobulins, Intravenous - therapeutic use
Interleukin-2 - secretion
Lymph Nodes - cytology
Lymphocyte Activation
Male
Rats
Rats, Inbred BN
Rats, Inbred Lew
Research Support, Non-U.S. Gov't
Retinitis - immunology - prevention & control
Tuberculin - immunology
Uveitis - immunology - prevention & control
Abstract
We have evaluated the effect of human Igs for intravenous use (IVIg) on the onset and development of experimental autoimmune uveoretinitis (EAU), a T cell-dependent autoimmune disease induced in rats by a single immunization with retinal S-antigen (S-Ag). Five consecutive daily infusions of IVIg, starting on the same day as S-Ag immunization, protected (Lewis x Brown-Norway) F1 rats against EAU. The prevention of EAU was IVIg-specific, i.e. mediated by pooled human IgG from multiple donors, since neither infusions of BSA nor infusions of pooled Ig from only two healthy individuals were effective. Treatment with IVIg decreased lymphocyte proliferative and antibody responses to S-Ag and the proliferative response to concanavalin A. Lack of proliferation was not dependent upon generation of suppressor cells. Lymph node (LN) cells from IVIg-treated and S-Ag-immunized animals neither proliferated nor secreted IL-2 in response to S-Ag but proliferated when co-cultured with LN cells from rats immunized with S-Ag. Our findings are compatible with an induction of a state of functional inactivation/anergy of T lymphocytes by infusions of IVIg. This functional inactivation may be due to the presence in IVIg of antibodies that bind both in vivo and in vitro to rat lymphocytes. Results from the present study suggest a novel mechanism by which IVIg may be beneficial in human autoimmune diseases.
PubMed ID
8312226 View in PubMed
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Immune parameters in a longitudinal study of a very old population of Swedish people: a comparison between survivors and nonsurvivors.

https://arctichealth.org/en/permalink/ahliterature214016
Source
J Gerontol A Biol Sci Med Sci. 1995 Nov;50(6):B378-82
Publication Type
Article
Date
Nov-1995
Author
F G Ferguson
A. Wikby
P. Maxson
J. Olsson
B. Johansson
Author Affiliation
Department of Veterinary Science, Pennsylvania State University, USA.
Source
J Gerontol A Biol Sci Med Sci. 1995 Nov;50(6):B378-82
Date
Nov-1995
Language
English
Publication Type
Article
Keywords
Aged
Aged, 80 and over
Aging - immunology
Antigens, CD - analysis
Cell Division - drug effects
Cluster analysis
Concanavalin A - pharmacology
Humans
Immune System - physiology
Longitudinal Studies
Lymphocyte Count - drug effects
Lymphocyte Subsets - cytology
Retrospective Studies
Survival Analysis
Survivors
Sweden
T-Lymphocytes - cytology - drug effects - immunology
Abstract
As a part of an ongoing longitudinal investigation, this study examined relationships between survival and selected immune system parameters in a sample (n = 102) of very old individuals (86-92 years at the time of initial immune system data collection). Analyses were performed comparing initial time-point measurements from those individuals who were alive (n = 75) and those who were deceased (n = 27) two years after initial data collection. Immune system measurements consisted of determination of peripheral blood lymphocytes and lymphocyte subsets, as well as T-cell responses to activation by Concanavalin A. Cluster analysis identified a subgroup associated with nonsurvival which indicated characteristics that included: poor T-cell proliferative responses, high CD8 percentages, and low CD4 and CD19 percentages. This multivariate analysis suggested that combinations of immune system parameters predict two-year survival otherwise not apparent when single immune system parameters were evaluated in the elderly.
PubMed ID
7583794 View in PubMed
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[Immunopathophysiologic characteristics of early pregnancy in women with recurrent miscarriage]

https://arctichealth.org/en/permalink/ahliterature63201
Source
Fiziol Zh. 2005;51(2):65-72
Publication Type
Article
Date
2005
Author
T V Radysh
V P Chernyshov
Author Affiliation
Institute of Pediatrics, Obstetrics and Gynecology, Academy of Medical Science of Ukraine, Kyiv.
Source
Fiziol Zh. 2005;51(2):65-72
Date
2005
Language
Ukrainian
Publication Type
Article
Keywords
Abortion, Habitual - blood - immunology
Antigens, CD - immunology
Biological Markers - blood
Case-Control Studies
Concanavalin A - pharmacology
English Abstract
Female
HLA-DR Antigens - immunology
Humans
Immunologic Memory - immunology
In Vitro
Lymphocyte Activation - drug effects - immunology
Pregnancy
T-Lymphocyte Subsets - drug effects - immunology
T-Lymphocytes, Helper-Inducer - drug effects - immunology
T-Lymphocytes, Regulatory - drug effects - immunology
Abstract
The lymphocyte subsets, activation marker expression and activity of ConA-treated lymphocytes have been studied in 58 patients with a history of unexplainable pregnancy loss (UPL) and 22 normal pregnant women (control) in 4-7 weeks of pregnancy. The increase of CD16/56+ cell level and CD4+/ CD8+ ratio and decrease of CD19+ cell level have been found in peripheral blood of UPL patients in comparison with control. The expression of HLA-DR was upregulated on CD3+ and CD8+ cells and the expression of CD25 was downregulated on CD3+, CD4+, CD8+, and CD16/56+ cells in UPL women. According to correlation analysis results, low expression of CD25 was related to a low expression of CD8 on NK, high expression of CD45RA on CD4+ helper cells, high expression of HLA-DR on CD8+ cytotoxic cells. High frequency of ConA-induced activation, low frequency of ConA-induced suppression and low suppressive activity of ConA-induced lymphocyte were found in UPL patients compared to control. Conclusion: women with UPL have disorders in feto-maternal recognition in early pregnancy that led to a development of the inadequate immune response to fetus realized as a defect of NK activation, deficiency of T-cytotoxic cell limitation and memory helper cell generation, downregulation of TR cells and suppressor function.
PubMed ID
15943233 View in PubMed
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16 records – page 1 of 2.