Skip header and navigation

3 records – page 1 of 1.

Abyssivirga alkaniphila gen. nov., sp. nov., an alkane-degrading, anaerobic bacterium from a deep-sea hydrothermal vent system, and emended descriptions of Natranaerovirga pectinivora and Natranaerovirga hydrolytica.

https://arctichealth.org/en/permalink/ahliterature275915
Source
Int J Syst Evol Microbiol. 2016 Apr;66(4):1724-34
Publication Type
Article
Date
Apr-2016
Author
Anders Schouw
Tove Leiknes Eide
Runar Stokke
Rolf Birger Pedersen
Ida Helene Steen
Gunhild Bødtker
Source
Int J Syst Evol Microbiol. 2016 Apr;66(4):1724-34
Date
Apr-2016
Language
English
Publication Type
Article
Keywords
Alkanes - metabolism
Arctic Regions
Bacterial Typing Techniques
Base Composition
Biodegradation, Environmental
Clostridiales - classification - genetics - isolation & purification
DNA, Bacterial - genetics
Fatty Acids - chemistry
Glycolipids - chemistry
Hydrothermal Vents - microbiology
Molecular Sequence Data
Peptidoglycan - chemistry
Phospholipids - chemistry
Phylogeny
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Abstract
A strictly anaerobic, mesophilic, syntrophic, alkane-degrading strain, L81T, was isolated from a biofilm sampled from a black smoker chimney at the Loki's Castle vent field. Cells were straight, rod-shaped, Gram-positive-staining and motile. Growth was observed at pH?6.2-9.5, 14-42?°C and 0.5-6?% (w/w) NaCl, with optima at pH?7.0-8.2, 37?°C and 3% (w/w) NaCl. Proteinaceous substrates, sugars, organic acids and hydrocarbons were utilized for growth. Thiosulfate was used as an external electron acceptor during growth on crude oil. Strain L81T was capable of syntrophic hydrocarbon degradation when co-cultured with a methanogenic archaeon, designated strain LG6, isolated from the same enrichment. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain L81T is affiliated with the family Lachnospiraceae, and is most closely related to the type strains of Natranaerovirga pectinivora (92?% sequence similarity) and Natranaerovirga hydrolytica (90%). The major cellular fatty acids of strain L81T were C15?:?0, anteiso-C15?:?0 and C16?:?0, and the profile was distinct from those of the species of the genus Natranaerovirga. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, three unidentified phospholipids, four unidentified glycolipids and two unidentified phosphoglycolipids. The G+C content of genomic DNA was determined to be 31.7?mol%. Based on our phenotypic, phylogenetic and chemotaxonomic results, strain L81T is considered to represent a novel species of a new genus of the family Lachnospiraceae, for which we propose the name Abyssivirga alkaniphila gen. nov., sp. nov. The type strain of Abyssivirga alkaniphila is L81T (=DSM 29592T=JCM 30920T). We also provide emended descriptions of Natranaerovirga pectinivora and Natranaerovirga hydrolytica.
PubMed ID
26822139 View in PubMed
Less detail

Alkaliphilus namsaraevii sp. nov., an alkaliphilic iron- and sulfur-reducing bacterium isolated from a steppe soda lake.

https://arctichealth.org/en/permalink/ahliterature285198
Source
Int J Syst Evol Microbiol. 2017 Jun;67(6):1990-1995
Publication Type
Article
Date
Jun-2017
Author
Anastasiya Zakharyuk
Lyudmila Kozyreva
Elena Ariskina
Olga Troshina
Dmitry Kopitsyn
Viktoria Shcherbakova
Source
Int J Syst Evol Microbiol. 2017 Jun;67(6):1990-1995
Date
Jun-2017
Language
English
Publication Type
Article
Keywords
Alkalies
Bacterial Typing Techniques
Base Composition
Clostridiales - classification - genetics - isolation & purification
DNA, Bacterial - genetics
Fatty Acids - chemistry
Ferric Compounds - metabolism
Lakes - microbiology
Phylogeny
RNA, Ribosomal, 16S - genetics
Russia
Sequence Analysis, DNA
Sulfur
Sulfur-Reducing Bacteria - classification - genetics - isolation & purification
Abstract
A novel alkaliphilic spore-forming bacterium was isolated from the benthic sediments of the highly mineralized steppe Lake Khilganta (Transbaikal Region, Russia). Cells of the strain, designated ?-07-2T, were straight to slightly curved rods, Gram-stain-positive and motile. Strain ?-07-2T grew in the pH range from 7.0 to 10.7 (optimum pH 9.6-10.3). Growth was observed at 25-47?°C (optimum 30?°C) and at an NaCl concentration from 5 to 150?g l-1 with an optimum at 40?g l-1. Strain ?-07-2T was a chemo-organoheterotroph able to reduce amorphous ferric hydroxide, Fe(III) citrate and elemental sulfur in the presence of yeast extract as the electron donor. It used tryptone, peptone and trypticase with Fe(III) citrate as the electron acceptor. The predominant fatty acids in cell walls were C16:1?8, iso-C15:0, C14?:?0 3-OH and C16?:?0. The DNA G+C content was 32.6?mol%. 16S rRNA gene sequence analysis revealed that strain ?-07-2T was related most closely to members of the genus Alkaliphilus within the family Clostridiaceae. The closest relative was Alkaliphilus peptidifermentans Z-7036T (96.4?% similarity). On the basis of the genotypic, chemotaxonomic and phenotypic data, strain ?-07-2T represents a novel species in the genus Alkaliphilus, for which the name Alkaliphilus namsaraevii sp. nov. is proposed. The type strain is ?-07-2T (=VKM ?-2746?=DSM 26418?).
PubMed ID
28632119 View in PubMed
Less detail

Ruthenibacterium lactatiformans gen. nov., sp. nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces.

https://arctichealth.org/en/permalink/ahliterature281950
Source
Int J Syst Evol Microbiol. 2016 Aug;66(8):3041-9
Publication Type
Article
Date
Aug-2016
Author
Andrei N Shkoporov
Andrei V Chaplin
Victoria A Shcherbakova
Natalia E Suzina
Lyudmila I Kafarskaia
Vladimir K Bozhenko
Boris A Efimov
Source
Int J Syst Evol Microbiol. 2016 Aug;66(8):3041-9
Date
Aug-2016
Language
English
Publication Type
Article
Keywords
Adult
Bacterial Typing Techniques
Base Composition
Child, Preschool
Clostridiales - classification - genetics - isolation & purification
DNA, Bacterial - genetics
Fatty Acids - chemistry
Feces - microbiology
Gram-Positive Bacteria - genetics
Humans
Lactic Acid - biosynthesis
Male
Phylogeny
RNA, Ribosomal, 16S - genetics
Russia
Sequence Analysis, DNA
Abstract
Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585-1T and 668, were characterized by mesophilic fermentative metabolism, production of d-lactic acid, succinic acid and acetic acid as end products of d-glucose fermentation, prevalence of C18?:?1?9, C18?:?1?9 aldehyde, C16?:?0 and C16?:?1?7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4-56.6 mol%. The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6?% similarity with Anaerofilum pentosovorans FaeT, 91.3/91.2?% with Gemmiger formicilis ATCC 27749T and 88.9/88.8?% with Faecalibacterium prausnitzii ATCC 27768T. On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae, for which the name Ruthenibacterium lactatiformans gen. nov., sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585-1T (=DSM 100348T=VKM B-2901T).
PubMed ID
27154556 View in PubMed
Less detail