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12 records – page 1 of 2.

A 1-year evaluation of Syva MicroTrak Chlamydia enzyme immunoassay with selective confirmation by direct fluorescent-antibody assay in a high-volume laboratory.

https://arctichealth.org/en/permalink/ahliterature217461
Source
J Clin Microbiol. 1994 Sep;32(9):2208-11
Publication Type
Article
Date
Sep-1994
Author
E L Chan
K. Brandt
G B Horsman
Author Affiliation
Laboratory and Disease Control Services, Saskatchewan Health, Regina, Canada.
Source
J Clin Microbiol. 1994 Sep;32(9):2208-11
Date
Sep-1994
Language
English
Publication Type
Article
Keywords
Algorithms
Chlamydia Infections - diagnosis - epidemiology - microbiology
Chlamydia trachomatis - immunology - isolation & purification
Cost Control
Densitometry
Diagnostic Tests, Routine - economics
Evaluation Studies as Topic
Female
Fluorescent Antibody Technique - economics
Humans
Immunoenzyme Techniques - economics
Male
Predictive value of tests
Prevalence
Reagent kits, diagnostic
Saskatchewan - epidemiology
Seasons
Sensitivity and specificity
Urethritis - diagnosis - epidemiology - microbiology
Uterine Cervicitis - diagnosis - epidemiology - microbiology
Abstract
TThe Syva MicroTrak Chlamydia enzyme immunoassay (EIA; Syva Company, San Jose, Calif.) with cytospin and direct fluorescent-antibody assay (DFA) confirmation was evaluated on 43,630 urogenital specimens over a 1-year period in the Provincial Laboratory in Regina, Saskatchewan, Canada. This was a two-phase study intended to define a testing algorithm for Chlamydia trachomatis that would be both highly accurate and cost-effective in our high-volume (> 3,000 tests per month) laboratory. The prevalence of C. trachomatis infection in our population is moderate (8 to 9%). In phase 1, we tested 6,022 male and female urogenital specimens by EIA. All specimens with optical densities above the cutoff value and those within 30% below the cutoff value were retested by DFA. This was 648 specimens (10.8% of the total). A total of 100% (211 of 211) of the specimens with optical densities equal to or greater than 1.00 absorbance unit (AU) above the cutoff value, 98.2% (175 of 178) of the specimens with optical densities of between 0.500 and 0.999 AU above the cutoff value, and 83% (167 of 201) of the specimens with optical densities within 0.499 AU above the cutoff value were confirmed to be positive. A total of 12% (7 of 58) of the specimens with optical densities within 30% below the cutoff value were positive by DFA. In phase 2, we tested 37,608 specimens (32,495 from females; 5,113 from males) by EIA. Only those specimens with optical densities of between 0.499 AU above and 30% below the cutoff value required confirmation on the basis of data from phase 1 of the study. This was 4.5% of all specimens tested. This decrease in the proportion of specimens requiring confirmation provides a significant cost savings to the laboratory. The testing algorithm gives us a 1-day turnaround time to the final confirmed test results. The MicroTrak EIA performed very well in both phases of the study, with a sensitivity, specificity, positive predictive value, and negative predictive value of 96.1, 99.1, 90.3, and 99.7%, respectively, in phase 2. We suggest that for laboratories that use EIA for Chlamydia testing, a study such as this one will identify an appropriate optical density range for confirmatory testing for samples from that particular population.
Notes
Cites: Epidemiol Rev. 1983;5:96-1236357824
Cites: J Clin Microbiol. 1993 Jun;31(6):1646-78315010
Cites: Diagn Microbiol Infect Dis. 1992 Nov-Dec;15(8):663-81478048
Cites: J Clin Microbiol. 1990 Nov;28(11):2473-62254422
PubMed ID
7814548 View in PubMed
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Childhood trachoma in a nonendemic area. Danish trachoma patients and their close contacts, 1963 to 1973.

https://arctichealth.org/en/permalink/ahliterature41692
Source
JAMA. 1978 Apr 28;239(17):1765-71
Publication Type
Article
Date
Apr-28-1978
Author
C H Mordhorst
S P Wang
J T Grayston
Source
JAMA. 1978 Apr 28;239(17):1765-71
Date
Apr-28-1978
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Age Factors
Antibodies, Bacterial - analysis
Child
Chlamydia trachomatis - immunology - isolation & purification
Conjunctiva - microbiology
Denmark
Female
Follow-Up Studies
Humans
Male
Middle Aged
Recurrence
Trachoma - epidemiology - transmission
Urethra - microbiology
Vagina - microbiology
Abstract
During ten years of study of Chlamydia trachomatis eye infections, trachoma was diagnosed in 14 Danish patients with onset during childhood. Clinical findings in the eye were characteristic of classical trachoma. The infecting C trachomatis immunotype was identified in all but one case. At the time of diagnosis, seven patients were still children (6 to 10 years of age), three were teenagers, and four were adults. In five young girls the disease was extremely severe. Delay in proper diagnosis and adequate therapy contributed to the prolongation and severity of the disease. Failure to diagnose is attributed to widespread opinion and teaching of physicians in Western countries that trachoma eye disease has disappeared. Studies of family members and other contacts of the patients contributed to an understanding of the pathogenesis of this disease. The source of initial eye infection with C trachomatis organisms in these cases was thought to be the birth canal. It was further postulated that reinfection of the eyes of these children occurred either from a reservoir in their own or their mother's genital tract.
PubMed ID
633586 View in PubMed
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Chlamydia trachomatis infection in patients with laparoscopically verified acute salpingitis. Results of isolation and antibody determinations.

https://arctichealth.org/en/permalink/ahliterature65837
Source
Am J Obstet Gynecol. 1980 Dec 1;138(7 Pt 2):960-4
Publication Type
Article
Date
Dec-1-1980
Author
K T Ripa
L. Svensson
J D Treharne
L. Weström
P A Mårdh
Source
Am J Obstet Gynecol. 1980 Dec 1;138(7 Pt 2):960-4
Date
Dec-1-1980
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Antibodies, Bacterial - analysis
Cervix Uteri - microbiology
Chlamydia trachomatis - immunology - isolation & purification
Female
Humans
Immunoglobulin G - analysis
Immunoglobulin M - analysis
Laparoscopy
Lymphogranuloma Venereum - immunology
Middle Aged
Pregnancy
Salpingitis - etiology - immunology
Abstract
Culture and serology studies have shown Chlamydia trachomatis (CT) to be one of the causes of acute salpingitis (AS). In the present investigation, results of cervical cultures were correlated with serum antibody titers to CT in patients with laparoscopically verified AS. Serum samples from 206 patients, including paired sera from 80, were assayed. Of 206 patients, 118 had chlamydial IgG antibody titers of 1:64 or more. Patients with negative cultures for CT and an IgG titer of 1:64 or more had a significantly higher geometric mean titer than corresponding patients with positive cultures. In paired sera, a seroconversion or a fourfold or greater rise in IgG titer to CT was demonstrated in 35%, while a further 11% had detectable IgM antibody in a titer of 1:8 or more. The overall isolation frequency of CT was 33%, compared with 19% for Neisseria gonorrhoeae.
PubMed ID
6451178 View in PubMed
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Cytokine polymorphisms and severity of tubal damage in women with Chlamydia-associated infertility.

https://arctichealth.org/en/permalink/ahliterature151594
Source
J Infect Dis. 2009 May 1;199(9):1353-9
Publication Type
Article
Date
May-1-2009
Author
Ohman H
Tiitinen A
Halttunen M
Lehtinen M
Paavonen J
Surcel H-M
Author Affiliation
National Institute for Health and Welfare, Oulu, Finland.
Source
J Infect Dis. 2009 May 1;199(9):1353-9
Date
May-1-2009
Language
English
Publication Type
Article
Keywords
Adult
Antibodies, Fungal - blood
Antibody formation
Chaperonin 60 - immunology
Chlamydia Infections - genetics - immunology - pathology
Chlamydia trachomatis - immunology - isolation & purification
Codon - genetics
Cytokines - genetics
Fallopian Tubes - microbiology - pathology
Female
Fertilization in Vitro
Finland
Genetic Predisposition to Disease
Humans
Immunity, Cellular
Immunoglobulin G - blood
Infertility, Female - genetics - microbiology - pathology
Interferon-gamma - genetics
Interleukin-10 - genetics
Introns
Polymorphism, Genetic
Promoter Regions, Genetic
Transforming Growth Factor beta1 - genetics
Tumor Necrosis Factor-alpha - genetics
Young Adult
Abstract
Chronic inflammation induced by Chlamydia trachomatis can lead to tubal factor infertility (TFI). To investigate the genetic basis of chlamydial TFI and various manifestations of tubal damage, we studied functional polymorphisms in selected cytokine genes (IL-10 -1082 A/G, -819 T/C, and -592 A/C; IFN-gamma +874 T/A; TNF-alpha -308 G/A; TGF-beta1 codons 10 T/C and 25 G/C; and IL-6 -174 G/C) in 114 women with laparoscopically verified TFI (hereafter known as "cases") and in 176 controls. Evidence of past infection with C. trachomatis was demonstrated in 96 cases by use of a combined test for humoral and cell-mediated immune responses to chlamydial elementary bodies (EBs) and chlamydial heat-shock protein 60 antigens. We found that the IL-10 -1082 AA genotype and the TNF-alpha -308 A allele increased the risk of severe tubal damage in women with infertility associated with C. trachomatis (odds ratio [OR], 7.3 [95% confidence interval {CI}, 1.3-42] and 4.0 [95% CI, 1.0-16], respectively), suggesting that differences in these genes contribute to the wide spectrum of disease manifestations.
PubMed ID
19358670 View in PubMed
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Detection of Chlamydia trachomatis antigens in first-void urine to identify asymptomatic male carriers.

https://arctichealth.org/en/permalink/ahliterature223438
Source
APMIS. 1992 Jul;100(7):645-9
Publication Type
Article
Date
Jul-1992
Author
M. Genç
Y. Dutertre
M. Björk
P A Mårdh
Author Affiliation
Uppsala University Centre for STD Research, Sweden.
Source
APMIS. 1992 Jul;100(7):645-9
Date
Jul-1992
Language
English
Publication Type
Article
Keywords
Adult
Antigens, Bacterial - urine
Carrier State - diagnosis - urine
Chlamydia Infections - diagnosis - urine
Chlamydia trachomatis - immunology - isolation & purification
Humans
Immunoenzyme Techniques
Male
Predictive value of tests
Sensitivity and specificity
Sweden
Urethritis - diagnosis - urine
Abstract
Early morning first-void urine collected from 279 sexually active Swedish male recruits (mean age 19.5 years) was tested by two commercial enzyme immunoassay (EIA) kits, MicroTrak and IDEIA III, and by MicroTrak direct fluorescence assay (DFA), to detect Chlamydia trachomatis antigens. A result was assumed to be true-positive when any of the two non-culture tests were positive for the same specimen. In one case where only DFA was positive, confirmatory chlamydial testing was performed by isolating the organism from a urethral swab. On these premises, the number of true-positive men was 26 (9.3% of all men studied). The sensitivity, specificity, positive predictive value and negative predictive value for MicroTrak EIA were 85%, 98%, 85%, and 98%, respectively. IDEIA III was less sensitive than MicroTrak EIA (42% vs 85%). In conclusion, the diagnosis of asymptomatic chlamydial infections in men can be established with reasonable accuracy by the detection of Chlamydia antigens in urine samples using MicroTrak EIA.
PubMed ID
1642853 View in PubMed
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[Frequency of different infectious agents persistence in mononuclear leukocytes of blood and synovial fluid in patients with rheumatoid arthritis]

https://arctichealth.org/en/permalink/ahliterature13690
Source
Lik Sprava. 2005 Jul-Sep;(5-6):28-32
Publication Type
Article
Author
A V Petrov
Source
Lik Sprava. 2005 Jul-Sep;(5-6):28-32
Language
Ukrainian
Publication Type
Article
Keywords
Antibodies, Bacterial - analysis
Antigens, Bacterial - analysis
Antigens, Viral - analysis
Arthritis, Rheumatoid - blood
Chlamydia trachomatis - immunology - isolation & purification
Comparative Study
Cytomegalovirus - immunology - isolation & purification
DNA, Bacterial - analysis
DNA, Viral - analysis
English Abstract
Female
Fluorescent Antibody Technique, Indirect
Hepacivirus - immunology - isolation & purification
Hepatitis B virus - immunology - isolation & purification
Herpes Simplex - classification - immunology
Herpesvirus 4, Human - immunology - isolation & purification
Humans
Infection - complications - microbiology - virology
Leukocytes, Mononuclear - immunology - microbiology - virology
Male
Mycoplasma arthritidis - immunology - isolation & purification
Mycoplasma fermentans - immunology - isolation & purification
Polymerase Chain Reaction
RNA, Viral - analysis
Retrospective Studies
Synovial Fluid - immunology - microbiology - virology
Ukraine - epidemiology
Ureaplasma urealyticum - immunology - isolation & purification
Abstract
The study of persistence in mononuclear leukocytes (ML) of blood and synovial fluid of 218 patients with rheumatoid arthritis (RA) Cytornegalovirus (CMV), the 1-st and 2-nd types of Herpes virus simplex (VH), Epstain-Barr virus (VEB), Mycoplasma arthritidis (Ma), Mycoplasma fermentans (Mf), Ureaplasma urealiticum (U), Chlamidia trachomatis (Ct), viruses of Hepatitis B and C was carry out by direct and indirect immunofruorescence, immunoenzymatic analysis and polymerase chain reaction. An increased frequency of contamination of blood ML with infectious agents in patients with RA was established (57,4% compared with 16,7% in control group). The following infectious agents were revieled more frequently: in ML of blood and synovial fluid the Ma (relatively 20,5% and 15,9%), Mf (15,6% and 13,2%), Ct (18,4% and 13,2%), VH (27,1% and 10,5%), VEB (12,7% and 5,3%) and CMV (11,2% and 7,9%). Types of frequency dynamics of ML contamination with these infectious agents in different time phases of RA were determined.
PubMed ID
16396287 View in PubMed
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Frequency of ocular chlamydial infection among young adults.

https://arctichealth.org/en/permalink/ahliterature224413
Source
Can J Ophthalmol. 1992 Feb;27(1):16-8
Publication Type
Article
Date
Feb-1992
Author
K D Smith
A C Tokarewicz
B C Schieven
Author Affiliation
Department of Ophthalmology, University of Western Ontario, London.
Source
Can J Ophthalmol. 1992 Feb;27(1):16-8
Date
Feb-1992
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Antibodies, Bacterial - analysis
Chlamydia Infections - blood - epidemiology
Chlamydia trachomatis - immunology - isolation & purification
Eye Infections, Bacterial - blood - epidemiology
Female
Humans
Male
Ontario - epidemiology
Prospective Studies
Abstract
Young adults are at particular risk for Chlamydia trachomatis infection. To determine whether there is a high rate of asymptomatic ocular chlamydial infection in this population, 131 eyes from 72 patients aged 18 to 30 years with no symptoms of conjunctivitis were tested for C. trachomatis by means of McCoy cell culture and a direct enzyme immunoassay. In addition, 51 of the patients underwent serologic testing to detect systemic chlamydial disease. Ocular chlamydial infection was not found in any of the patients, including the 26 with a positive result of serologic testing. We conclude that routine screening of young adults for ocular chlamydial infection would be of no benefit in detecting systemic chlamydial infection and its sequelae.
PubMed ID
1555129 View in PubMed
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Lack of evidence of Chlamydia pneumoniae infection in infants with acute lower respiratory tract disease.

https://arctichealth.org/en/permalink/ahliterature220011
Source
Eur J Clin Microbiol Infect Dis. 1993 Nov;12(11):850-3
Publication Type
Article
Date
Nov-1993
Author
S M Yeung
K. McLeod
S P Wang
J T Grayston
E E Wang
Author Affiliation
Department of Pediatrics, Hospital for Sick Children, Toronto, Canada.
Source
Eur J Clin Microbiol Infect Dis. 1993 Nov;12(11):850-3
Date
Nov-1993
Language
English
Publication Type
Article
Keywords
Acute Disease
Antibodies, Bacterial - blood
Bronchiolitis - epidemiology - microbiology
Canada - epidemiology
Case-Control Studies
Chlamydia Infections - epidemiology - microbiology
Chlamydia trachomatis - immunology - isolation & purification
Chlamydophila pneumoniae - immunology - isolation & purification
Female
Humans
Immunoglobulin G - blood
Infant
Infant, Newborn
Male
Pneumonia - epidemiology - microbiology
Prevalence
Abstract
In order to determine whether there is serologic evidence of Chlamydia pneumoniae infection in young infants with acute lower respiratory tract infection, serum samples from 86 subjects aged less than 6 months were assayed for IgG and IgM antibodies to Chlamydia pneumoniae using a microimmunofluorescence method. Infants hospitalized in Toronto, Canada, were enrolled between 15 March 1991 and 15 March 1992. No patient had infection determined by the serologic results. IgG antibody was detected at low concentrations in 32 patients, with an inverse relationship between titer and chronological age. In our setting, Chlamydia pneumoniae does not appear to be an important lower respiratory pathogen in young infants.
PubMed ID
8112356 View in PubMed
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Lifetime number of partners as the only independent risk factor for human papillomavirus infection: a population-based study.

https://arctichealth.org/en/permalink/ahliterature23303
Source
Sex Transm Dis. 1995 Mar-Apr;22(2):119-27
Publication Type
Article
Author
R. Karlsson
M. Jonsson
K. Edlund
M. Evander
A. Gustavsson
E. Bodén
E. Rylander
G. Wadell
Author Affiliation
Department of Family Medicine, University of Umeå, Sweden.
Source
Sex Transm Dis. 1995 Mar-Apr;22(2):119-27
Language
English
Publication Type
Article
Keywords
Adult
Antibodies, Bacterial - blood
Antibodies, Viral - blood
Cervix Uteri - virology
Chlamydia Infections - microbiology
Chlamydia trachomatis - immunology - isolation & purification
Cohort Studies
Comparative Study
DNA, Viral - analysis
Female
Herpes Genitalis - virology
Herpesvirus 2, Human - immunology
Humans
Male
Papillomavirus, Human - genetics - isolation & purification
Papovaviridae Infections - epidemiology - psychology - virology
Polymerase Chain Reaction
Prevalence
Research Support, Non-U.S. Gov't
Risk factors
Sex Factors
Sexual Behavior
Sexual Partners
Sexually Transmitted Diseases, Viral - epidemiology - psychology - virology
Sweden - epidemiology
Tumor Virus Infections - epidemiology - psychology - virology
Abstract
BACKGROUND AND OBJECTIVES: Previous studies of relationships between genital human papillomavirus infection and tentative risk factors have yielded conflicting results, possibly because of inaccuracy of the viral detection methods used and differences in selection criteria. GOAL OF THIS STUDY: To determine human papillomavirus prevalence and identify risk factors in a group of young Swedish women. STUDY DESIGN: This was a population-based study involving completion of a structured questionnaire, analysis of cervical scrapings for human papillomavirus and Chlamydia trachomatis, and serologic tests for C. trachomatis and herpes simplex virus antibodies. RESULTS: The prevalence of human papillomavirus infection was 22% among the sexually active women and 4% among the virgins. A number of factors were associated with human papillomavirus prevalence in univariate analysis, but logistic regression analysis showed that lifetime number of male sexual partners was the only independent risk factor for human papillomavirus infection (adjusted odds ratio, 7.45; 95% CI, 2.79-19.92 for six or more partners vs. one partner). CONCLUSION: Human papillomavirus infection is a prevalent sexually transmitted disease among young Swedish women, and the lifetime number of male sexual partners is a major risk factor.
PubMed ID
7624813 View in PubMed
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[Serodiagnosis of chlamydiosis: "Chlamy-IgG-DS-Tr"-- the first domestic immunoenzyme recombinant test-system for determination of anti-Chlamydia trachomatis class G antibodies].

https://arctichealth.org/en/permalink/ahliterature197248
Source
Antibiot Khimioter. 2000;45(8):21-4
Publication Type
Article
Date
2000
Author
I N Manzeniuk
M S Vorob'eva
N M Nikitiuk
S A Fedosov
M A Gorbunov
S N Gnedoi
V I Losev
N A Krivenchuk
Author Affiliation
L.A. Tarasevich State Research Institute of Standartization and Drugs Controle, Moscow.
Source
Antibiot Khimioter. 2000;45(8):21-4
Date
2000
Language
Russian
Publication Type
Article
Keywords
Antibodies, Bacterial - immunology
Chlamydia Infections - blood - diagnosis - immunology - microbiology
Chlamydia trachomatis - immunology - isolation & purification
Humans
Immunoenzyme Techniques
Immunoglobulin G - analysis
Recombinant Proteins - immunology
Russia
Sensitivity and specificity
Serologic Tests
Abstract
The first Native test-system for determination of class G antibodies to Chlamydia trachomatis with chlamydia recombinant antigen was elaborated Test-system efficacy was demonstrated in clinical trials. The sensibility, specific activity and suitability of the "Chlamy-IgG-DS-Tr" were the same as for import analogous.
PubMed ID
10989720 View in PubMed
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12 records – page 1 of 2.