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Antibodies against RNA hydrolyze RNA and DNA.

https://arctichealth.org/en/permalink/ahliterature92322
Source
J Mol Recognit. 2008 Sep-Oct;21(5):338-47
Publication Type
Article
Author
Krasnorutskii Michael A
Buneva Valentina N
Nevinsky Georgy A
Author Affiliation
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, pr. Lavrent'eva 8, Novosibirsk, Russia.
Source
J Mol Recognit. 2008 Sep-Oct;21(5):338-47
Language
English
Publication Type
Article
Keywords
Animals
Antibodies, Antinuclear - pharmacology
Antibodies, Catalytic - metabolism - pharmacology
Cellulose - analogs & derivatives - pharmacology
DNA - metabolism - pharmacology
Deoxyribonucleases - metabolism
Hydrolysis - drug effects
Immunoglobulin G - metabolism - pharmacology
RNA - immunology - metabolism
Rabbits
Ribonucleases - metabolism
Abstract
Immunization of animals with DNA leads to the production of anti-DNA antibodies (Abs) demonstrating both DNase and RNase activities. It is currently not known whether anti-RNA Abs can possess nuclease activities. In an attempt to address this question, we have shown that immunization of three rabbits with complex of RNA with methylated BSA (mBSA) stimulates production of IgGs with RNase and DNase activities belonging to IgGs, while polyclonal Abs from three non-immunized rabbits and three animals immunized with mBSA are catalytically inactive. Affinity chromatography of IgGs from the sera of autoimmune (AI) patients on DNA-cellulose usually demonstrates a number of fractions, all of which effectively hydrolyze both DNA and RNA, while rabbit catalytic IgGs were separated into Ab subfractions, some of which demonstrated only DNase activity, while others hydrolyzed RNA faster than DNA. The enzymic properties of the RNase and DNase IgGs from rabbits immunized with RNA distinguish them from all known canonical RNases and DNases and DNA- and RNA-hydrolyzing abzymes (Abzs) from patients with different AI diseases. In contrast to RNases and AI RNA-hydrolyzing Abs, rabbit RNase IgGs catalyze only the first step of the hydrolysis reaction but cannot hydrolyze the formed terminal 2',3'-cyclophosphate. The data indicate that Abzs of AI patients hydrolyzing nucleic acids in part may be Abs against RNA and its complexes with proteins.
PubMed ID
18729241 View in PubMed
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[Development of an optimal enteric-soluble film-forming composite based on acetylphthalylcellulose for coating tablets in a fluidized bed]

https://arctichealth.org/en/permalink/ahliterature8886
Source
Farm Zh. 1975 Mar-Apr;(2):47-51
Publication Type
Article

[Development of the optimal film-forming composition based on ethylcellulose for the protective coating of tablets on a fluidized bed]

https://arctichealth.org/en/permalink/ahliterature13216
Source
Farm Zh. 1976 Mar-Apr;(2):65-8
Publication Type
Article

Influence of emulsifying agents on the properties of cellulose acetate butyrate and ethylcellulose microcapsules.

https://arctichealth.org/en/permalink/ahliterature11502
Source
J Microencapsul. 1994 Nov-Dec;11(6):633-9
Publication Type
Article
Author
T. Kristmundsdóttir
K. Ingvarsdóttir
Author Affiliation
Department of Pharmacy, University of Iceland, Reykjavík.
Source
J Microencapsul. 1994 Nov-Dec;11(6):633-9
Language
English
Publication Type
Article
Keywords
Capsules
Cellulose - analogs & derivatives - chemistry
Chemistry, Pharmaceutical - methods
Excipients - pharmacology
Ibuprofen - administration & dosage - chemistry
Polysorbates - chemistry - pharmacology
Polyvinyl Alcohol - chemistry - pharmacology
Research Support, Non-U.S. Gov't
Abstract
Microcapsules of ibuprofen were prepared according to the solvent evaporation method, using two coating polymers, cellulose acetate butyrate and ethylcellulose. The influence of two emulsifying agents, polysorbat 80 and polyvinyl alcohol, on the properties of the microcapsules was investigated. The results show that type and concentration of emulsifying agent influences size distribution, drug loading and amount of free drug on the surface of the microcapsules.
PubMed ID
7884628 View in PubMed
Less detail

[Isolation and purification of nonspecific nuclease of cyanobacterium Plectonema boryanum CALU 465]

https://arctichealth.org/en/permalink/ahliterature9486
Source
Mikrobiol Z. 2004 Jan-Feb;66(1):29-35
Publication Type
Article
Author
N V Tsymbal
V A Samoilenko
S A Syrchin
M I Mendzhul
Author Affiliation
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Source
Mikrobiol Z. 2004 Jan-Feb;66(1):29-35
Language
Russian
Publication Type
Article
Keywords
Cellulose - analogs & derivatives
Centrifugation
Cyanobacteria - enzymology
Deoxyribonucleases - chemistry - isolation & purification
English Abstract
Ethanolamines
Ion Exchange
Molecular Weight
Sucrose
Abstract
Nonspecific nuclease has been isolated from the cells of cyanobacterium Plectonema boryanum and purified to homogenic state. It has been established that the method of centrifugation of cell-free culture extract in the sucrose density gradient is efficient for the separation of pigment proteins and enzyme concentration. Under the successive use of two ion-exchangers the nuclease activity was determined in the concentration range of NaCl 0.065-0.085 M after separation of the cell-free cyanobacterium extract on the column with phosphocellulose in the range of 0.2-0.25 M, on the column with DEAE--Toyopearl respectively. The molecular mass of nuclease which is 40 kDa, has been determined by electrophoresis in polyacrylamide gel under denaturating conditions and gel-filtration on Sephadex G-100. It has been also established that the given enzyme is monosubunitary as to its structure.
PubMed ID
15104052 View in PubMed
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Some consequences of using cigarette machine smoking regimes with different intensities on smoke yields and their variability.

https://arctichealth.org/en/permalink/ahliterature139330
Source
Regul Toxicol Pharmacol. 2011 Mar;59(2):293-309
Publication Type
Article
Date
Mar-2011
Author
Stephen W Purkis
Xavier Cahours
Miguel Rey
Beatrice Teillet
Valerie Troude
Thomas Verron
Author Affiliation
Imperial Tobacco Limited, Southville, Bristol, UK. steve.purkis@uk.imptob.com
Source
Regul Toxicol Pharmacol. 2011 Mar;59(2):293-309
Date
Mar-2011
Language
English
Publication Type
Article
Keywords
Canada
Carbon Monoxide - analysis - chemistry
Cellulose - analogs & derivatives
Filtration
Humans
Nicotine - analysis - chemistry
Smoke - analysis
Smoking
Tars - analysis - chemistry
Temperature
Ventilation
Water
Abstract
When smoking cigarettes under an intense regime with a combination of 100% ventilation blocking and high flow rates, as currently mandated by Health Canada, significant increases in filter temperatures and disproportionately high levels of mainstream smoke water and moisture accumulating in the spent filter were found when compared to other smoking regimes, especially for highly filter ventilated cigarettes. These effects have been reported to decrease cigarette firmness during the course of smoking, to alter filtration properties and efficiencies and to confound the measurement of particulate matter. The high filter temperatures generated also lead to significant amounts of vapour phase compounds desorbing from carbon filters and an over-estimation of the yields of these components. Less adsorption on or more desorption from carbon filters was found for compounds with the highest volatility. Therefore, yield data from the intense regime may not reflect the effectiveness of cigarette design features to reduce certain smoke components that occurs when products are smoked under conditions closer to those used by the majority of smokers in real world situations. In addition, a combination of these interacting factors may explain the worse level of between-laboratory reproducibility data for particulate matter measurement obtained during intense machine smoking. Among-laboratory data variability for vapour phase components, other than carbon monoxide, and for particulate phase components, other than nicotine, still needs to be evaluated in collaborative studies. Before proposing smoking regimes as tools to evaluate smoke emissions, it is essential to understand these various interacting factors and subsequent uncontrolled effects that such regimes can generate and the limitations of their use. These observations imply that higher tolerances may need to be set and taken into account when smoking under the intense regime before deciding that, for a given product, there are real differences between the yields determined in different laboratories.
PubMed ID
21074590 View in PubMed
Less detail

Tablets with high lactose content: effects of some binders and disintegrants on their properties.

https://arctichealth.org/en/permalink/ahliterature7115
Source
Pharmazie. 2005 Sep;60(9):711-2
Publication Type
Article
Date
Sep-2005
Author
R. Eyjolfsson
Author Affiliation
Drug Development, Hafnarfjordur, Iceland.
Source
Pharmazie. 2005 Sep;60(9):711-2
Date
Sep-2005
Language
English
Publication Type
Article
Keywords
Cellulose - analogs & derivatives - chemistry
Chemistry, Pharmaceutical
Excipients
Lactose - chemistry
Pharmaceutic Aids - chemistry
Povidone - chemistry
Starch
Tablets
Abstract
Tablets containing 91% lactose monohydrate and 4% starch pregelatinized exhibited the best technical properties in comparison to tablets of similar composition with either povidone plus croscarmellose sodium or hydroxypropylcellulose low substituted as binders/disintegrants.
PubMed ID
16222875 View in PubMed
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[The gas chromatographic determination of sulfur- and oxygen-containing organic compounds released into the air of cellulose sulfate works]

https://arctichealth.org/en/permalink/ahliterature11945
Source
Gig Tr Prof Zabol. 1992;(7):34-5
Publication Type
Article
Date
1992
Author
N A Taranenko
V B Dorogova
Source
Gig Tr Prof Zabol. 1992;(7):34-5
Date
1992
Language
Russian
Publication Type
Article
Keywords
Air Pollutants, Occupational - analysis
Cellulose - analogs & derivatives
Chromatography, Gas - instrumentation - methods
Disulfides - analysis
English Abstract
Reproducibility of Results
Siberia
Sulfides - analysis
Wood
Abstract
The article presents data on the design of sensitive, selective, useful in group analysis method to detect dimethylsulphide, dimethyldisulphide, acetic, propionic, butyric and valeric acids, methyl alcohol and phenol by means of gas chromatography in the air of cellulose sulphate production working zone. The methods were tested in examining the work conditions in Bratsk found-lavage shops.
PubMed ID
1296914 View in PubMed
Less detail

8 records – page 1 of 1.