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Adoptively transferred late allergic airway responses are associated with Th2-type cytokines in the rat.

https://arctichealth.org/en/permalink/ahliterature57629
Source
Am J Respir Cell Mol Biol. 1997 Jan;16(1):69-74
Publication Type
Article
Date
Jan-1997
Author
A. Watanabe
H. Mishima
T C Kotsimbos
M. Hojo
P M Renzi
J G Martin
Q A Hamid
Author Affiliation
Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.
Source
Am J Respir Cell Mol Biol. 1997 Jan;16(1):69-74
Date
Jan-1997
Language
English
Publication Type
Article
Keywords
Adoptive Transfer
Airway Resistance
Animals
Bronchoalveolar Lavage Fluid - cytology - immunology
CD4-Positive T-Lymphocytes - immunology - transplantation
CD8-Positive T-Lymphocytes - immunology - transplantation
Interferon Type II - biosynthesis
Interleukin-2 - biosynthesis
Interleukin-4 - biosynthesis
Interleukin-5 - biosynthesis
Interleukins - biosynthesis
Ovalbumin - immunology
Rats
Research Support, Non-U.S. Gov't
Respiratory Hypersensitivity - immunology
Th2 Cells - immunology
Abstract
Late allergic airway responses can be transferred by CD4+ T cells in the rat. To investigate the role of T-cell cytokines in these responses, we examined the expression of mRNA for Th2 (interleukin [IL]-4 and IL-5) and Th1 (IL-2 and interferon gamma [INF-gamma])-type cytokines in Brown Norway rats that were administered either antigen-primed W3/25(CD4)+ or OX8(CD8)+ T cells. Donors were actively sensitized by subcutaneous injection of ovalbumin (OVA) in the neck and T cells were obtained from the cervical lymph nodes by immunomagnetic cell sorting for administration to unsensitized rats. Control rats received bovine serum albumin (BSA)-primed CD4+ and CD8+ T cells. Two days later, recipient rats were challenged with aerosolized OVA, and bronchoalveolar lavage (BAL) was performed 8 h after challenge. BAL cells expressing mRNA for IL-2, IL-4, IL-5, and INF-gamma were analyzed using the technique of in situ hybridization. Recipients of OVA-primed CD4+ T cells had an increase in the fraction of BAL cells expressing mRNA for IL-4 and IL-5 compared with BSA-primed CD4+ or OVA-primed CD8+ cells (P
PubMed ID
8998081 View in PubMed
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Adoptive transfer of allergen-specific CD4+ T cells induces airway inflammation and hyperresponsiveness in brown-Norway rats.

https://arctichealth.org/en/permalink/ahliterature57604
Source
Immunology. 1997 Jun;91(2):176-85
Publication Type
Article
Date
Jun-1997
Author
A. Haczku
P. Macary
T J Huang
H. Tsukagoshi
P J Barnes
A B Kay
D M Kemeny
K F Chung
R. Moqbel
Author Affiliation
Department of Allergy and Clinical Immunology, Guy's Hospital, London, UK.
Source
Immunology. 1997 Jun;91(2):176-85
Date
Jun-1997
Language
English
Publication Type
Article
Keywords
Adoptive Transfer
Animals
Bronchial Hyperreactivity - immunology
Bronchoalveolar Lavage Fluid - immunology
CD4-Positive T-Lymphocytes - immunology - transplantation
CD8-Positive T-Lymphocytes - immunology - transplantation
Cell Culture Techniques
Cell Division - immunology
Eosinophils - immunology
Leukocyte Count
Lymphocyte Transfusion
Male
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Spleen - immunology
Abstract
Following allergen exposure, sensitized Brown-Norway rats develop airway hyperresponsiveness (AHR) and eosinophilic inflammation together with an increase in activated T cells (CD25+) in the airways. We tested the hypothesis that CD4+ T cells are involved directly in the acquisition of AHR. Spleen T cells from animals that were injected intraperitoneally on three consecutive days with ovalbumin/Al(OH)3, showed a dose-dependent proliferative response in vitro to ovalbumin, but not to bovine serum albumin, as measured by [3H]thymidine uptake. For total T-cell transfer, spleen cells obtained from donor rats 4 days after sensitization were depleted of adherent cells by a nylon wool column separation. CD4+ and CD8+ T cells were purified by immunomagnetic beads cell separation. Recipient naive rats were injected intravenously with 50 x 10(6) total T cells, 20 x 10(6) and 5 x 10(6) CD4+ cells, and 5 x 10(6) CD8+ cells, and were exposed to ovalbumin aerosol 24 hr afterwards. After a further 24 hr, airway responsiveness to acetylcholine (ACh) was measured and provocative concentration (PC) values PC100, PC200 and PC300) (the ACh concentration needed to achieve 100, 200 and 300% increase in lung resistance above baseline) were calculated. Airway responsiveness was significantly increased in recipients of sensitized total T cells compared with recipients of cells from saline-injected donor rats (P
PubMed ID
9227314 View in PubMed
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Allogeneic intrabone marrow-bone marrow transplantation plus donor lymphocyte infusion suppresses growth of colon cancer cells implanted in skin and liver of rats.

https://arctichealth.org/en/permalink/ahliterature78890
Source
Stem Cells. 2007 Feb;25(2):385-91
Publication Type
Article
Date
Feb-2007
Author
Koike Yasushi
Adachi Yasushi
Suzuki Yasuhiro
Iwasaki Masayoshi
Koike-Kiriyama Naoko
Minamino Keizo
Nakano Keiji
Mukaide Hiromi
Shigematsu Akio
Kiyozuka Yasuhiko
Tubura Airo
Kamiyama Yasuo
Ikehara Susumu
Author Affiliation
First Department of Pathology, Kansai Medical University, 10-15 Moriguchi, Osaka, Japan.
Source
Stem Cells. 2007 Feb;25(2):385-91
Date
Feb-2007
Language
English
Publication Type
Article
Keywords
Animals
Bone Marrow Transplantation
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Cell Proliferation
Colonic Neoplasms - pathology
Killer Cells, Natural - immunology
Liver - pathology
Lymphocyte Transfusion
Rats
Rats, Inbred BN
Rats, Inbred F344
Skin - pathology
Spleen - cytology - immunology
Survival Rate
Transplantation, Homologous
Xenograft Model Antitumor Assays
Abstract
We have recently found that allogeneic intrabone marrow-bone marrow transplantation (IBM-BMT) + donor lymphocyte infusion (DLI) using CD4(+) cell-depleted spleen cells (CD4(-) cells) can prevent graft-versus-host disease (GvHD) but suppress tumor growth (Meth A: fibrosarcoma) in mice. In the present study, we show that allogeneic IBM-BMT + DLI using CD4(-) cells also has suppressive effects on the growth of colon cancer cells implanted not only in the skin but also in the liver of rats. First, we examined the effects of allogeneic IBM-BMT + DLI on the subcutaneously inoculated ACL-15 (rat colon cancer cell line). Lethally irradiated Fischer rats (F344 rats) were transplanted with T-cell-depleted bone marrow cells (BMCs) from Brown Norway (BN) rats. Simultaneously, DLI was performed using whole spleen cells (whole cells), CD4(+) cell-depleted spleen cells (CD4(-) cells) or CD8(+) cell-depleted spleen cells (CD8(-) cells) of BN rats. Although allogeneic IBM-BMT + DLI suppressed tumor growth, a considerable number of rats treated with allogeneic IBM-BMT + DLI using whole cells or CD8(-) cells died due to GvHD. In contrast, allogeneic IBM-BMT + DLI using CD4(-) cells also suppressed tumor growth, but there was no GvHD. Based on these findings, we next examined the effects of allogeneic IBM-BMT + DLI using CD4(-) cells on the cancer cells implanted in the liver. Allogeneic IBM-BMT + DLI using CD4(-) cells via the portal vein significantly prolonged the survival. These results suggest that allogeneic IBM-BMT + DLI using CD4(-) cells could become a new strategy for the treatment of solid tumors.
PubMed ID
17284650 View in PubMed
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Anti-CD8 treatment reduces the severity of inflammatory arthritis, but not vasculitis, in mercuric chloride-induced autoimmunity.

https://arctichealth.org/en/permalink/ahliterature14277
Source
Clin Exp Immunol. 1996 Nov;106(2):280-5
Publication Type
Article
Date
Nov-1996
Author
P D Kiely
D. O'Brien
D B Oliveira
Author Affiliation
Division of Renal Medicine, St George's Hospital Medical School, London, UK.
Source
Clin Exp Immunol. 1996 Nov;106(2):280-5
Date
Nov-1996
Language
English
Publication Type
Article
Keywords
Animals
Antibodies, Monoclonal - pharmacology
Antigens, CD8 - immunology
Arthritis - chemically induced - immunology - prevention & control
Autoantibodies - analysis
Autoimmunity - drug effects
CD8-Positive T-Lymphocytes - immunology
Cecal Diseases - chemically induced - immunology
Collagen - immunology
Enzyme-Linked Immunosorbent Assay
Immunoglobulin E - analysis
Interferon Type II - blood
Killer Cells, Natural - immunology
Lymphocyte Depletion
Lymphocyte Subsets - immunology
Mercuric Chloride - toxicity
Peroxidase - immunology
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Vasculitis - chemically induced - immunology
Abstract
Mercuric chloride (HgCl2) induces a T cell-dependent autoimmune syndrome in Brown-Norway (BN) rats characterized by a humoral response, tissue injury with an accumulation of CD8+ and CD4+ T cells, and an increase in tissue IL-4 mRNA and serum IgE suggesting Th2 cell activation. In other models of autoimmune disease, CD8+ cells act in both anti- and pro-inflammatory capacities, suggesting that functionally distinct CD8+ populations exist in vivo. The effect of treatment with OX8, a depleting anti-CD8 MoAb, on the initiation of HgCl2-induced autoimmunity was assessed in two experiments in a total of 20 BN rats, and compared with 20 animals treated with a control MoAb or PBS. OX8 significantly depleted peripheral blood CD8+ lymphocytes, had no effect on HgCl2-induced anti-collagen or myeloperoxidase antibodies, nor on the incidence or severity of caecal vasculitis. The severity of HgCl2-induced arthritis was significantly reduced in OX8-treated animals; median peak score reduced from 7.5 to 3.0 (experiment 1) and from 7.0 to 4 (experiment 2) (P = 0.009, Mann-Whitney U-test). OX8 treatment also exacerbated the early rise in HgCl2-induced IgE and induced a significant rise in plasma interferon-gamma (IFN-gamma), suggesting that CD8+ cells may have a regulatory influence on Th cell populations. These data provide direct evidence that CD8+ cells may act in a proinflammatory capacity in both this model of autoimmunity and the pathogenesis of inflammatory arthritis.
PubMed ID
8918574 View in PubMed
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Antigen-specific beta-chemokine production and CD8 T-cell noncytotoxic antiviral activity in HIV-2-infected individuals.

https://arctichealth.org/en/permalink/ahliterature7177
Source
Scand J Immunol. 2005 Jan;61(1):63-71
Publication Type
Article
Date
Jan-2005
Author
R K S Ahmed
H. Norrgren
Z. da Silva
A. Blaxhult
E-L Fredriksson
G. Biberfeld
S. Andersson
R. Thorstensson
Author Affiliation
Tumorbiology Center, Karolinska Institute, Solna, Sweden. raija.ahmed@smi.ki.se
Source
Scand J Immunol. 2005 Jan;61(1):63-71
Date
Jan-2005
Language
English
Publication Type
Article
Keywords
Adult
CD8-Positive T-Lymphocytes - immunology
Chemokines, CC - biosynthesis
Comparative Study
Female
Guinea-Bissau
HIV Antigens
HIV Infections - immunology - virology
HIV-1 - immunology - pathogenicity - physiology
HIV-2 - immunology - pathogenicity - physiology
Humans
In Vitro
Macrophage Inflammatory Protein-1 - biosynthesis
Male
Middle Aged
RANTES - biosynthesis
Research Support, Non-U.S. Gov't
Sweden
Virus Replication
Abstract
Human immunodeficiency virus-2 (HIV-2) is less pathogenic than HIV-1, and the disease progression in HIV-2-infected individuals seems to be similar to that seen in HIV-1-infected long-term nonprogressors. Cell-mediated immune responses and the production of noncytotoxic CD8+ T-cell antiviral factors (CAF) and beta-chemokines have been correlated to protection against HIV-1 and associated with asymptomatic infection and slower disease progression. We investigated the antigen-induced beta-chemokine production in HIV-2-infected patients living in Sweden and in Guinea-Bissau. We also compared in vitro CD8+ T-cell-mediated noncytotoxic antiviral activity against beta-chemokine-sensitive R5 virus (HIV-1Bal) and beta-chemokine-insensitive X4 virus (HIV-1IIIB) in HIV-2-infected patients with that in HIV-1-infected patients. HIV-2-specific beta-chemokine production was demonstrated in a majority of the HIV-2-infected subjects. CD8+ T cells of both HIV-1 and HIV-2-infected individuals suppressed R5 virus replication in vitro in a similar manner, while the inhibition of X4 virus replication seemed to be more frequent and of a higher magnitude among HIV-2-infected patients compared to HIV-1-infected subjects. Taken together, our results indicate that the production of CD8+ T-cell noncytotoxic antiviral factors may contribute to the low transmission of the virus and slower disease progression in HIV-2-infected patients.
PubMed ID
15644124 View in PubMed
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Association between CD8+ T-cell subsets and cardiovascular disease.

https://arctichealth.org/en/permalink/ahliterature116822
Source
J Intern Med. 2013 Jul;274(1):41-51
Publication Type
Article
Date
Jul-2013
Author
D. Kolbus
I. Ljungcrantz
L. Andersson
B. Hedblad
G N Fredrikson
H. Björkbacka
J. Nilsson
Author Affiliation
Department of Clinical Sciences Malmö, Lund University, Sweden.
Source
J Intern Med. 2013 Jul;274(1):41-51
Date
Jul-2013
Language
English
Publication Type
Article
Keywords
Aged
Antigens, CD56 - analysis
Biological Markers - blood
CD8-Positive T-Lymphocytes - immunology
Cardiovascular Diseases - immunology
Carotid Intima-Media Thickness
Carotid Stenosis - epidemiology - immunology - physiopathology - ultrasonography
Cohort Studies
Coronary Artery Disease - epidemiology - immunology - physiopathology - ultrasonography
Cytokines - secretion
Female
Flow Cytometry
Humans
Incidence
Interleukin-2 Receptor alpha Subunit - analysis
Kaplan-Meier Estimate
Leukocytes - immunology
Male
Middle Aged
Odds Ratio
Predictive value of tests
Proportional Hazards Models
Prospective Studies
Registries
Risk assessment
Risk factors
Severity of Illness Index
Stroke - epidemiology - immunology - physiopathology - ultrasonography
Sweden - epidemiology
Abstract
The findings of experimental studies suggest that the immune system plays a key role in atherosclerosis, but the clinical importance of different immune cells in cardiovascular disease remains poorly characterized. In this study we investigated the association between CD8(+) T cells and carotid disease as well as development of cardiovascular disease events.
The study cohort comprised 700 subjects from the cardiovascular arm of the Malmö Diet and Cancer Study. Peripheral blood mononuclear cells, obtained at the 1991-1994 baseline investigation and stored at -140 °C, were thawed and the different CD8(+) T-cell populations analysed by flow cytometry. Baseline carotid intima-media thickness and stenosis were assessed by ultrasonography and clinical events were monitored through validated national registers.
Subjects with a high fraction of CD8(+) T cells were characterized by decreased cytokine release from activated leucocytes, metabolic signs of insulin resistance and increased incidence of coronary events; hazard ratios (95% confidence intervals) for the second and third tertiles of CD8(+) T cells were 2.57 (1.16, 5.67) and 2.61 (1.19, 5,71), respectively, in a Cox proportional hazards regression model. Correlations were found between the fraction of CD8(+) CD25(+) T cells and the degree of carotid stenosis (r = 0.11, P
PubMed ID
23356723 View in PubMed
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CD4+ T cells can induce airway hyperresponsiveness to allergen challenge in the brown norway rat.

https://arctichealth.org/en/permalink/ahliterature57554
Source
Am J Respir Crit Care Med. 1998 Dec;158(6):1863-70
Publication Type
Article
Date
Dec-1998
Author
H. Mishima
M. Hojo
A. Watanabe
Q A Hamid
J G Martin
Author Affiliation
Meakins-Christie Laboratories, Royal Victoria Hospital, McGill University, and the Respiratory Health Network of Centres of Excellence, Montreal, Quebec, Canada.
Source
Am J Respir Crit Care Med. 1998 Dec;158(6):1863-70
Date
Dec-1998
Language
English
Publication Type
Article
Keywords
Aerosols
Allergens - immunology
Animals
Bronchial Hyperreactivity - immunology
Bronchial Provocation Tests
Bronchoalveolar Lavage Fluid - chemistry - immunology
Bronchoconstrictor Agents - diagnostic use
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Cattle
Comparative Study
Eosinophilia - immunology
Gene Expression Regulation
Immunization
Immunoglobulin E - immunology
In Situ Hybridization
Interferon Type II - genetics - immunology
Interleukin-5 - genetics - immunology
Male
Methacholine Chloride - diagnostic use
Ovalbumin - immunology
Proteins - analysis
RNA, Messenger - genetics
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Serum Albumin, Bovine - immunology
Time Factors
Abstract
Airway hyperresponsiveness to inhalational challenge with methacholine (MCh) develops by 32 h after allergen challenge of actively sensitized BN rats. To test the hypothesis that CD4+ T cells mediate allergen-induced hyperresponsiveness independent of IgE-mediated mechanisms, we administered CD4+ T cells, CD8+ T cells, and a mixture of CD4+ and CD8+ T cells (total T cells) isolated from the cervical lymph nodes of rats sensitized with ovalbumin (OA) to naive BN rats that underwent aerosol challenge with either OA or bovine serum albumin (BSA) 2 d later. Responsiveness to MCh was measured 2 d before transfer of T cells and 32 h after challenge with OA or BSA. Airway responsiveness increased significantly in recipients of CD4+ T cells after OA challenge, but not in any other of the treatment groups. Analysis of bronchoalveolar lavage (BAL) cells for major basic protein expression by immunostaining showed eosinophilia in OA-challenged CD4+ and total T-cell recipients. Cells retrieved by bronchoalveolar lavage showed increased expression of IL-5 mRNA (in situ hybridization) in CD4+ T cell recipients after OA challenge compared with other groups. Interferon-gamma mRNA was expressed to the greatest extent in CD8+ recipients, but it was elevated in both OA- and BSA-challenged animals. We conclude that CD4+ T cells can induce airway hyperresponsiveness after inhalational challenge with allergen and this is associated with IL-5 production and eosinophilia. CD8+ T cells may have a negative regulatory effect on responsiveness, possibly mediated by interferon-gamma.
PubMed ID
9847279 View in PubMed
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CD8+ alphabeta T cells can mediate late airway responses and airway eosinophilia in rats.

https://arctichealth.org/en/permalink/ahliterature57391
Source
J Allergy Clin Immunol. 2004 Dec;114(6):1345-52
Publication Type
Article
Date
Dec-2004
Author
Susumu Isogai
Rame Taha
Meiyo Tamaoka
Yasuyuki Yoshizawa
Qutayba Hamid
James G Martin
Author Affiliation
Department of Medicine, McGill University, Montreal, Quebec, Canada.
Source
J Allergy Clin Immunol. 2004 Dec;114(6):1345-52
Date
Dec-2004
Language
English
Publication Type
Article
Keywords
Adoptive Transfer
Animals
Bronchoalveolar Lavage Fluid - immunology
CD8-Positive T-Lymphocytes - immunology
Cytokines - biosynthesis
Eosinophilia - etiology
Lung Diseases - etiology
Male
Ovalbumin - immunology
Rats
Rats, Inbred BN
Receptors, Antigen, T-Cell, alpha-beta - analysis
Research Support, Non-U.S. Gov't
Abstract
BACKGROUND: The function of CD8+ T-cell subsets in mediating late allergic responses is incompletely understood. OBJECTIVE: We sought to test the hypothesis that CD8+ alphabeta T cells are proinflammatory in the airways in vivo by using a well-characterized animal model and the technique of adoptive transfer. METHODS: Brown Norway rats were administered CD8 + alphabeta T cells (10 6 ) intraperitoneally purified from lymph node cells of either naive or ovalbumin (OVA)-sensitized rats and were challenged with aerosolized OVA 2 days later. Control rats were sensitized to 100 mug of OVA in Al(OH) 3 subcutaneously or sham sensitized to saline and were OVA challenged 2 weeks later. RESULTS: The OVA-sensitized and OVA-challenged group and the recipients of OVA-primed CD8+ alphabeta T cells had significant late airway responses calculated from lung resistance measured for an 8-hour period after challenge compared with the naive CD8 + alphabeta T cell-transferred group and the sham-sensitized control group. The number of eosinophils in bronchoalveolar lavage fluid increased in the OVA-sensitized group and the OVA-primed CD8+ alphabeta T-cell recipients compared with numbers in the naive CD8+ alphabeta T-cell recipients and the sham-sensitized control group. IL-4 and IL-5 cytokine mRNA expression in bronchoalveolar lavage fluid increased in the OVA-sensitized group and the OVA-primed CD8+ alphabeta T-cell recipients compared with that in the sham-sensitized group. CONCLUSION: We conclude that antigen-primed CD8 + alphabeta T cells might have a proinflammatory role in allergen-driven airway responses in the rat.
PubMed ID
15577833 View in PubMed
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CD8 positive T cells express IL-17 in patients with chronic obstructive pulmonary disease.

https://arctichealth.org/en/permalink/ahliterature135451
Source
Respir Res. 2011;12:43
Publication Type
Article
Date
2011
Author
Ying Chang
Jessica Nadigel
Nicholas Boulais
Jean Bourbeau
François Maltais
David H Eidelman
Qutayba Hamid
Author Affiliation
Meakins-Christie Laboratories and Respiratory Division, Department of Medicine McGill University, 3626 rue St, Urbain, Montreal, QC, H2X 2P2 Canada.
Source
Respir Res. 2011;12:43
Date
2011
Language
English
Publication Type
Article
Keywords
Adult
Biopsy
Bronchi - immunology
Bronchoscopy
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Case-Control Studies
Female
Humans
Immunohistochemistry
Immunophenotyping
Interleukin-17 - analysis - genetics
Lasers
Male
Microdissection - instrumentation
Middle Aged
Pulmonary Disease, Chronic Obstructive - diagnosis - genetics - immunology
Quebec
RNA, Messenger - analysis
Reverse Transcriptase Polymerase Chain Reaction
Severity of Illness Index
Abstract
Chronic obstructive pulmonary disease (COPD) is a progressive and irreversible chronic inflammatory disease of the lung. The nature of the immune reaction in COPD raises the possibility that IL-17 and related cytokines may contribute to this disorder. This study analyzed the expression of IL-17A and IL-17F as well as the phenotype of cells producing them in bronchial biopsies from COPD patients.
Bronchoscopic biopsies of the airway were obtained from 16 COPD subjects (GOLD stage 1-4) and 15 control subjects. Paraffin sections were used for the investigation of IL-17A and IL-17F expression in the airways by immunohistochemistry, and frozen sections were used for the immunofluorescence double staining of IL-17A or IL-17F paired with CD4 or CD8. In order to confirm the expression of IL-17A and IL-17F at the mRNA level, a quantitative RT-PCR was performed on the total mRNA extracted from entire section or CD8 positive cells selected by laser capture microdissection.
IL-17F immunoreactivity was significantly higher in the bronchial biopsies of COPD patients compared to control subjects (P
Notes
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PubMed ID
21477350 View in PubMed
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CD8+ T cells modulate late allergic airway responses in Brown Norway rats.

https://arctichealth.org/en/permalink/ahliterature57532
Source
J Immunol. 1999 Nov 15;163(10):5574-81
Publication Type
Article
Date
Nov-15-1999
Author
M. Suzuki
R. Taha
D. Ihaku
Q. Hamid
J G Martin
Author Affiliation
Meakins-Christie Laboratories, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
Source
J Immunol. 1999 Nov 15;163(10):5574-81
Date
Nov-15-1999
Language
English
Publication Type
Article
Keywords
Adoptive Transfer
Animals
Bronchial Provocation Tests
Bronchoalveolar Lavage Fluid - cytology - immunology
CD8-Positive T-Lymphocytes - immunology - metabolism - transplantation
Cytokines - biosynthesis
Flow Cytometry
Hypersensitivity, Delayed - immunology
Immunoglobulin E - blood
Leukocytes, Mononuclear - cytology - immunology
Lymph Nodes - cytology - immunology - metabolism
Lymphocyte Count
Lymphocyte Subsets - cytology - immunology
Male
Ovalbumin - blood - immunology
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Spleen - cytology - immunology - metabolism
Abstract
To test the hypothesis that CD8+ T cells may suppress the allergen-induced late airway response (LAR) and airway eosinophilia, we examined the effect of administration of Ag-primed CD8+ T cells on allergic airway responses, bronchoalveolar lavage (BAL) leukocytes, and mRNA expression for cytokines (IL-4, IL-5, and IFN-gamma) in OVA-sensitized Brown Norway rats. On day 12 postsensitization to OVA, test rats were administered 2 million CD8+ T cells i.p. isolated from either the cervical lymph nodes (LN group; n = 8) or the spleen (Spl group; n = 6) of sensitized donors. On day 14, test rats were challenged with aerosolized OVA. Control rats were administered PBS i.p. on day 12, and challenged with OVA (n = 10) or BSA (n = 6) on day 14. The lung resistance was measured for 8 h after challenge. BAL was performed at 8 h. Cytospin slides of BAL were analyzed for major basic protein by immunostaining and for cytokine mRNA by in situ hybridization. The LAR was significantly less in the LN group (1.8 +/- 0.5 U; p
PubMed ID
10553086 View in PubMed
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42 records – page 1 of 5.