Skip header and navigation

Refine By

2 records – page 1 of 1.

[Molecular genetics typing of Brucella circulating in several provinces of Mongolia].

https://arctichealth.org/en/permalink/ahliterature141341
Source
Zh Mikrobiol Epidemiol Immunobiol. 2010 May-Jun;(3):17-22
Publication Type
Article
Author
Iu K Kulakov
J. Erdenebaator
L E Tsirelson
T A Tolmacheva
M M Zheludkov
E I Korenberg
Source
Zh Mikrobiol Epidemiol Immunobiol. 2010 May-Jun;(3):17-22
Language
Russian
Publication Type
Article
Keywords
Animals
Bacterial Typing Techniques - methods
Brucella - classification - genetics - isolation & purification
Brucellosis - epidemiology - microbiology - veterinary
DNA Primers
DNA, Bacterial - genetics
Disease Reservoirs - microbiology - veterinary
Genetic Variation
Humans
Molecular Epidemiology
Mongolia - epidemiology
Ruminants - microbiology
Sensitivity and specificity
Abstract
Comparative molecular-genetic typing of Brucella strains isolated in Mongolia from different animal species as well as from humans.
Twenty-one strains of Brucella isolated from different hosts in 7 provinces of Mongolia were typed. Conventional phenotypic methods, genotyping by PCR with primers for genus- and species-specific differentiating targets of Brucella genes as well as multiple locus variable number tandem repeats analysis (MLVA) with 12 pairs of primers bounding locus variable tandem repeats of different length (from 134 bp to 8 bp).
Phenotypic identification and genotyping by PCR using primers for differentiating DNA markers allowed to attribute 14 isolates to B. melitensis biovar 2, and 7 - to B. abortus biovar 3. By using the MLVA method, connection of MLVA genotypes of 9 Brucella isolates with their reservoir hosts (sheep, cows) was shown providing their circulation in Khentii, Bulqan, and Khubsgul provinces bordering with Russia. Nine isolates from different hosts (camel, yaks, goats, sheep) isolated in Ovorkhangai, Dundgovi, and Dornogovi provinces, which have not border with Russia, had closely related MLVA genotypes indicating an opportunity of migration of pathogenic Brucella species to not-typical hosts.
Molecular-genetic typing of Brucella isolated in Mongolia was done for the first time; levels of their genetic relation and diversity were demonstrated. Circulation of Brucella isolated with specific MLVA genotypes was connected to territories of specific Mongolian provinces. The study proved migration of Brucella to not-typical hosts. Comparative study of isolates circulating in frontier with Mongolia areas of Russia (Irkutsk region, Tyva and Buryat Republics) are necessary to perform.
PubMed ID
20734715 View in PubMed
Less detail

Prevalence of Coxiella burnetii and Brucella spp. in tissues from subsistence harvested northern fur seals (Callorhinus ursinus) of St. Paul Island, Alaska.

https://arctichealth.org/en/permalink/ahliterature276865
Source
Acta Vet Scand. 2014;56:67
Publication Type
Article
Date
2014
Author
Colleen Duncan
Bobette Dickerson
Kristy Pabilonia
Amy Miller
Tom Gelatt
Source
Acta Vet Scand. 2014;56:67
Date
2014
Language
English
Publication Type
Article
Keywords
Alaska - epidemiology
Animals
Brucella - isolation & purification
Brucellosis - epidemiology - microbiology - veterinary
Coxiella burnetii - isolation & purification
DNA, Bacterial - analysis
Fur Seals
Prevalence
Q Fever - epidemiology - microbiology - veterinary
Real-Time Polymerase Chain Reaction - veterinary
Abstract
The northern fur seal (Callorhinus ursinus) is an important cultural and nutritional resource for the Aleut community on St. Paul Island Alaska. In recent years, an increasing number of zoonotic pathogens have been identified in the population, but the public health significance of these findings is unknown. To determine the prevalence of Coxiella burnetii and Brucella spp. in northern fur seal tissues, eight tissue types from 50 subsistence-harvested fur seals were tested for bacterial DNA by real-time polymerase chain reaction.
Of the 400 samples tested, only a single splenic sample was positive for Brucella spp. and the cycle threshold (ct) value was extremely high suggesting a low concentration of DNA within the tissue. C. burnetii DNA was not detected.
Findings suggest that the risk of humans contracting brucellosis or Q fever from the consumption of harvested northern fur seals is low.
Notes
Cites: Rev Sci Tech. 2013 Apr;32(1):89-10323837368
Cites: Acta Clin Belg. 2000 Jan-Feb;55(1):30-310783505
Cites: Occup Med (Lond). 1997 Sep;47(7):432-49374073
Cites: Vet Rec. 1999 Apr 24;144(17):48310358880
Cites: Clin Microbiol Rev. 1999 Oct;12(4):518-5310515901
Cites: QJM. 2005 Jan;98(1):7-2015625349
Cites: Scand J Infect Dis. 2006;38(11-12):1119-2217148093
Cites: J Clin Microbiol. 2006 Dec;44(12):4363-7017035490
Cites: Int J Surg Pathol. 2007 Apr;15(2):172-317478774
Cites: Am J Trop Med Hyg. 2009 Oct;81(4):691-419815888
Cites: J Wildl Dis. 2010 Apr;46(2):450-7320688638
Cites: Annu Rev Microbiol. 2011;65:523-4121939378
Cites: Vector Borne Zoonotic Dis. 2012 Mar;12(3):192-522017469
Cites: Clin Infect Dis. 2012 Nov 15;55(10):1387-922893578
Cites: J Wildl Dis. 2013 Jan;49(1):163-723307383
Cites: J Wildl Dis. 2013 Apr;49(2):441-623568925
Cites: Transbound Emerg Dis. 2013 Aug;60(4):345-5022747976
Cites: Epidemiol Infect. 2000 Jun;124(3):543-910982079
Cites: Emerg Infect Dis. 2003 Apr;9(4):485-812702232
PubMed ID
25266039 View in PubMed
Less detail