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Pulmonary function changes and immunomodulation of Th 2 cytokine expression induced by aminophylline after sensitization and allergen challenge in brown Norway rats.
Ann Allergy Asthma Immunol. 2002 Feb;88(2):215-22
Publication Type
Ching-Chi Lin
Ching-Yuang Lin
Shwu-Fang Liaw
Ann Chen
Author Affiliation
Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan.
Ann Allergy Asthma Immunol. 2002 Feb;88(2):215-22
Publication Type
Allergens - immunology
Aminophylline - pharmacology - therapeutic use
Asthma - drug therapy - immunology
Bronchial Hyperreactivity - drug therapy
Bronchoalveolar Lavage Fluid - immunology
Cytokines - genetics - metabolism
Lung - immunology - pathology - physiology
Ovalbumin - immunology
RNA, Messenger - metabolism
Research Support, Non-U.S. Gov't
Respiratory Function Tests
Th2 Cells - drug effects - immunology - metabolism
Theophylline - blood
BACKGROUND AND OBJECTIVE: Evidence has shown that aminophylline has bronchoprotective, anti-inflammatory, and immunomodulatory effects. Our purpose was to evaluate the effect of different doses of aminophylline on the late-phase reaction, bronchial hyperresponsiveness (BHR) and T cell-related cytokine mRNA expression in brown Norway rats induced by ovalbumin (OA) sensitization. METHODS: Forty rats were equally divided into four groups. Groups I, II, and III animals were sensitized and subsequently provoked with OA. Aminophylline 25 mg/kg was given intraperitoneally to the group I animals and 5 mg/kg to group II animals. Group III animals received intraperitoneal normal saline. Group IV breathed aerosolized saline as a control. After OA provocation, the animals were anesthetized. Pulmonary function tests were performed at baseline and after varying doses of acetylcholine. Thereafter, bronchoalveolar lavage was performed and the lungs were examined histologically. Total RNA was extracted from lung tissue and reverse transcriptase-polymerase chain reaction was performed using primers for interleukin (IL)-2, IL-4, IL-5, IL-6, IL-10, interferon-gamma, inducible nitric oxide synthase, and beta-actin. RESULTS: Group III had worse pulmonary function tests, more severe BHR, and more severe lung inflammation, higher IL-4 and IL-10 cytokine levels in bronchoalveolar lavage fluid, and higher IL-4, IL-5, IL-6, IL-10, tumor necrosis factor-alpha and inducible nitric oxide synthase mRNA expression than the other three groups. Expression of IL-2 and interferon-gamma was significantly reduced in group III. CONCLUSIONS: Both low and high dose aminophylline are effective in preventing late-phase bronchoconstriction, BHR, and an inflammatory response. Aminopylline decreases T helper cell 2-related cytokine mRNA expression but increases T helper cell 1-related cytokines mRNA expression.
PubMed ID
11868928 View in PubMed
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