Data are submitted on individual prophylaxis of damage to the genetical apparatus of bodily normal cells in cytostatic therapy of immunodeficiency states by observing changes in biochemical indices, such as activity of enzymes, content of biologically active substances and hormones.
Cases are described of a concomitant course of acute viral hepatitis B and obstructive jaundice. Particular features are considered of the associated versus singular course of the two conditions. Clinical, biochemical, and ultrasonic data are presented together with criteria for diagnosis. Recommendations are given on diagnosis and treatment of this category of patients.
We have determined a release of a stable mitochondrial factor (SMF) into the outflowing blood in vivo. That effect was induced by ischemia/reperfusion or phenylarsin oxide (PAO)--the activators of the mitochondrial permeability transition pore (MPTP) and the oxidative stress. The SMF was measured by a spectrophotometer in the range of wave-lengths 230-260 nm with the absorption maximum of 240-250 nm. The SMF release was accompanied with a decrease in the myocardial contractility, disturbances in cardiodynamics and regional blood circulation. The data obtained have demonstrated that the SMF can be used as a marker of MPTP opening in vivo.
The data on causes and rate of purulent-necrotic complications (PNC) occurrence of pancreatic cysts (PC) are adduced. Difficulties of these complications diagnosis and treatment were noted. Diagnostic markers for the PC PNC course and the treatment efficacy were proposed.
The model of ischemia/reperfusion was reproduced on 9 unconscious dogs. Simultaneously with registration of indexes heart work, hemodynamic and mitochondrial factor (MF) in venous blood from right atrium was defined. Measures were done by spectrophotometria. We have also performed spectrophotometric determination of MF in 11 patients in course of operation with blood cardioplegia. Samples of mixed venous blood from the right atrium were taken on different stages of artificial blood circulation: ischemia and reperfusion. Besides that, patients' level of enzymes was defined: creatine kinase (CK), mv-creatine kinase (mv-CK), lactatedehydrogenase (LDG), aspartataminotransferase (AST), within first day of postoperative period, and also ECG-recording within pre- and after operative period were done. Maximal MF level correlates with frequency and severity of cardiac rhythm disturbance, severity of myocardial hypoxia (r = 0.81). Maximum MF level also demonstrated correlation with KK (r = 0.97), mvKK (r = 0.92), LDG(r = 0.81), AST (r = 0.85). Thus, in experimental and clinic conditions myocardial ischemia was accompanied by mPTP activation, which led to reperfusion myocardial injuries and to release of MF. Method of MF determination gives opportunity to propose its usage as early marker of ischemic injuries and also as marker of mPTP opening in vivo.
[Effect of a complex treatment, including glutargin and erbisol on the blood plasma content of protein P53, apoptotic markers of type II, activity of caspases and the level of sCD 117 in patients with autonomic-vascular dystonia]
It has been established that the blood content of protein P53 diminishes by 27%, the blood level of sTRAIL increases by 22%, sCD 117 increases by 44% in patients with vegeto-vascular dystonia of the hypertonic type that is accompanied by an increase of the activity of caspases-1 however the activity of caspases-3 and - 8 as well as the blood content of TNF-alpha do not change. Multimodality therapy using glutargin does not influence on the level of the blood plasma TNF-alpha and the activity of caspases-1,-3,-8, normalizes the blood content of sTRAIL and sCD 117, however does not change the plasma concentration of protein P53 which remains lower by 35% than the control indices. In patients with vegeto-vascular dystonia of the hypotonic type the concentration of blood plasma protein P53, TNF-alpha and sTRAIL and the activity of caspases-1,-3,-8 correspond to the control values against a background of an almost twofold increase of the plasma sCD 117 level. The use of erbisol in a complex of therapeutic agents does not change the activity of caspases-l,-3,-8 and does not influence on the blood content of protein p53, TNF-alpha and sTRAIL and diminishes the plasma level of sCD 117 up to control values. A considerable elevation of the blood content of type II apoptotic factors is characteristic of the mixed type of vegeto-vascular dystonia: the level of protein p53 increases 2,4 times, TNF-alpha - 1,9 times, sTRAIL - 2,3 times that is accompanied by an increased activity of caspase-1 - 4,1 times, caspase-3 - 3,3 times, caspase-8 - 3,8 times and an increase of the plasma concentration of sCD 117 - 3,5 times. The use of erbisol and glutargin in multimodality therapy normalizes the plasma concentration of TNF-alpha and diminishes the blood content of protein P53 by 33% and sTRAIL - by 42% which, nevertheless remains higher than the control value by 58% and 36% respectively. The combined effects of glutargin and erbisol in patients of this group are characterized by a decrease (but not normalization) of the blood content of sCD 117 by 47% and more than twofold decrease of the activity of caspases-1,-3 and -8.
The lymphocyte subsets, activation marker expression and activity of ConA-treated lymphocytes have been studied in 58 patients with a history of unexplainable pregnancy loss (UPL) and 22 normal pregnant women (control) in 4-7 weeks of pregnancy. The increase of CD16/56+ cell level and CD4+/ CD8+ ratio and decrease of CD19+ cell level have been found in peripheral blood of UPL patients in comparison with control. The expression of HLA-DR was upregulated on CD3+ and CD8+ cells and the expression of CD25 was downregulated on CD3+, CD4+, CD8+, and CD16/56+ cells in UPL women. According to correlation analysis results, low expression of CD25 was related to a low expression of CD8 on NK, high expression of CD45RA on CD4+ helper cells, high expression of HLA-DR on CD8+ cytotoxic cells. High frequency of ConA-induced activation, low frequency of ConA-induced suppression and low suppressive activity of ConA-induced lymphocyte were found in UPL patients compared to control. Conclusion: women with UPL have disorders in feto-maternal recognition in early pregnancy that led to a development of the inadequate immune response to fetus realized as a defect of NK activation, deficiency of T-cytotoxic cell limitation and memory helper cell generation, downregulation of TR cells and suppressor function.
The impact of menopause and activity of rheumatoid inflammation on the osseous tissue metabolism was studied with the aid of biochemical markers of remodelling of the osseous tissue. Osteocalcin (noncollagenous protein of bone tissue) is believed to be the most informative marker of formation of the osseous tissue. In 70 female patients presenting with rheumatoid arthritis, blood serum concentration of osteocalcin was measured. Of these, 37 were in their premenopause, 33--in postmenopause. The control group was 26 female subjects. The activity of the rheumatoid process in premenopausal period and during the postmenopausal second and third five-year periods was found out to be associated with the rate of processes of bone tissue formation. At menopause the blood content of osteocalcin gets increased but subsequently it is noted to be on the decrease.
A comprehensive immunological evaluation was conducted together with phenotyping of haptoglobin (Hp) in 373 patients with freshly detected pulmonary tuberculosis. A marked suppression of T-cellular and antituberculous immunity has been shown to be the case in those patients presenting with Hp 2-2 phenotype of haptoglobin, which fact suggests to us a linkage between the bodily immunity status in patients with pulmonary tuberculosis and genetically determined blood marker.
Blood serum concentration of osteocalcin was studied in 53 patients with rheumatoid arthritis. Osteocalcin (noncollagenous protein of bony tissue) is a marker of bony tissue formation known to be of high informative value. There were no significant differences in values for osteocalcin concentration between patients with rheumatoid arthritis and healthy people. Reduction of osteocalcin content and, correspondingly, of process of bony tissue formation was observed in rapidly progressing course of rheumatoid arthritis and in stage IV arthritis. Rises in osteocalcin concentration were recordable in those women in there postmenopause. Patients with rheumatoid arthritis were found out to experience adequate formation of bony tissue in spite of the osteopenia syndrome progression. Disordered structural and functional state of the bony tissue processes of remodelling may be explained by bony tissue remodeling processes dissociation.