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[Antineoplastic properties of extracellular fructoso-1,6-bisphosphatase of Bacillus subtilis 668 and preparation isolated from cultural liquids of Bacillus subtilis 7025]

https://arctichealth.org/en/permalink/ahliterature83765
Source
Mikrobiol Z. 2007 May-Jun;69(3):68-73
Publication Type
Article
Author
Didenko H V
Dvorshchenko O S
Iastrebova O V
Potebnia M H
Panchenko L P
Skrypal' I H
Source
Mikrobiol Z. 2007 May-Jun;69(3):68-73
Language
Ukrainian
Publication Type
Article
Keywords
Animals
Antineoplastic Agents - isolation & purification - pharmacology
Apoptosis - drug effects
Bacillus subtilis - enzymology
Cell Line, Tumor
Culture Media - chemistry
Extracellular Fluid - enzymology
Fructose-Bisphosphatase - isolation & purification - pharmacology
Sarcoma 37 - pathology
Abstract
It was shown for the first time that extracellular FBPase of B. subtilis 668 like the preparation obtained from culture liquid of B. subtilis B 7025 displays citotoxicity activity in respect of tumor cells of sarcoma 37 in vitro. It is shown that the preparations remove TA antigens from the surface of the tumor cell. It is supposed that the mechanisms of citotoxic effect of extracellular FBFase of B. subtilis 668 and preparation from the culture liquid of B. subtilis B 7025 in vitro on cells of sarcoma 37 is probably realized through the apoptosis.
PubMed ID
17682534 View in PubMed
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Radiation technology in the preparation of polyethylene oxide hydrophilic gels and immobilization of proteases for use in medical practice.

https://arctichealth.org/en/permalink/ahliterature10192
Source
Arch Pharm Res. 2001 Jun;24(3):229-33
Publication Type
Article
Date
Jun-2001
Author
E I Vereschagin
D H Han
A W Troitsky
O V Grishin
S E Petrov
E P Gulyaeva
L A Bogdanova
M V Korobeinikov
V L Auslender
Author Affiliation
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk.
Source
Arch Pharm Res. 2001 Jun;24(3):229-33
Date
Jun-2001
Language
English
Publication Type
Article
Keywords
Bacillus subtilis - enzymology
Chemistry, Pharmaceutical
Chemistry, Physical
Endopeptidases - chemistry - radiation effects
Enzymes, Immobilized - chemistry - radiation effects
Gels
Molecular Weight
Oxidation-Reduction
Polyethylene Glycols - chemistry - radiation effects
Radiochemistry
Solutions
Abstract
This paper deals with the development of a technology for making a hydrophilic gel of polyethylene oxide reception in which radiating ability is employed to cause cross-linking of polymers in a water solution. The gel of polyethylene oxide was shown to be non-toxic, contain 5-50% of polymer and be useful in composite medicinal forms along with biologically active substances including Bac. subtilis proteases. Proteases immobilized in the gel possess high thermal stability and proteolytic activity and are readily applied in medicine. The effect of immobilized proteolytic and glucolytic enzymes of Bac. subtillis (Immozimase) on the warm ischemia-reperfusion (I/R) which can cause hepatic and jejunum injury was also studied. These enzymes were immobilized on water-soluble polymer polyethylene glycol by means of an electron beam. The number of degranulated mast cells as well as serum ALT after I/R in the group with Immozimase was decreased to almost half as compared with the control group. Pretreatment with Immozimase resulted in significant reduction of hepatic and gut neutrophil accumulation as compared with control animals. It was concluded that Immozimase has a protective effect for hepatic and gut ischemia/reperfusion, and this effect seems to be associated with prevention of leukocyte accumulation.
PubMed ID
11440083 View in PubMed
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