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Antigen challenge of sensitized rats increases airway responsiveness to methacholine.

https://arctichealth.org/en/permalink/ahliterature16166
Source
J Appl Physiol. 1988 Oct;65(4):1642-6
Publication Type
Article
Date
Oct-1988
Author
S. Bellofiore
J G Martin
Author Affiliation
Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.
Source
J Appl Physiol. 1988 Oct;65(4):1642-6
Date
Oct-1988
Language
English
Publication Type
Article
Keywords
Airway Resistance - drug effects
Animals
Antigens - immunology
Asthma - immunology - physiopathology
Immunization
Male
Methacholine Chloride
Methacholine Compounds - pharmacology
Ovalbumin - administration & dosage - immunology
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Abstract
We measured airway responsiveness to methacholine (MCh) of highly inbred rats before and after six inhalational challenges with antigen. Ten Brown-Norway rats (130-216 g) that were actively sensitized to ovalbumin (OA) received six challenges with OA at 5-day intervals beginning 19 days after sensitization. An aerosol of OA (5% wt/vol) was inhaled for 1, 2, 5, and 10 min or until pulmonary resistance (RL) increased by at least 50%. Challenges with aerosolized MCh were performed immediately before and 14 days after sensitization, 2 days after the 3rd OA exposure, and 2, 7, 12, and 17 days after the 6th OA challenge. Four unsensitized rats underwent inhalational challenges with MCh over an equivalent time period. Responsiveness to MCh was calculated as the concentration of MCh required to increase RL to 200% of the control value (EC200RL). Seven out of 10 rats in the experimental group reacted to the first OA challenge with an immediate increase in RL of greater than 50% of control (range 70-550%). Three animals were unreactive to OA. Base-line EC200RL for all rats undergoing sensitization was 2.13 mg/ml (geometric mean), and it did not change significantly after sensitization (2.05 mg/ml). However, EC200RL of the rats that reacted to OA (n = 7) decreased significantly after 3 (1.11 mg/ml; P less than 0.005) and 6 OA exposures (0.96 mg/ml; P less than 0.005). The increase in responsiveness to inhaled MCh was present 17 days after the last OA exposure (EC200RL = 1.40 mg/ml; P less than 0.05). EC200RL of neither the unreactive sensitized rats (n = 3) nor the control rats (n = 4) changed after OA challenges.(ABSTRACT TRUNCATED AT 250 WORDS)
PubMed ID
3053586 View in PubMed
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Cow's milk allergy, asthma, and pediatric IBD.

https://arctichealth.org/en/permalink/ahliterature117251
Source
J Pediatr Gastroenterol Nutr. 2013 Jun;56(6):649-51
Publication Type
Article
Date
Jun-2013
Author
Lauri J Virta
Merja Ashorn
Kaija-Leena Kolho
Author Affiliation
Department of Research, Social Insurance Institution, Turku, Finland.
Source
J Pediatr Gastroenterol Nutr. 2013 Jun;56(6):649-51
Date
Jun-2013
Language
English
Publication Type
Article
Keywords
Adolescent
Asthma - immunology - physiopathology
Case-Control Studies
Child
Child, Preschool
Colitis, Ulcerative - epidemiology - etiology - immunology
Crohn Disease - epidemiology - etiology - immunology
Female
Finland - epidemiology
Humans
Infant
Inflammatory Bowel Diseases - epidemiology - etiology - immunology
Male
Milk Hypersensitivity - immunology - physiopathology
Odds Ratio
Registries
Risk factors
Abstract
We identified a total of 595 Finnish children born in 1994-2008 and diagnosed as having inflammatory bowel disease (IBD) by October 2010 from the National Reimbursement Register (based on certificates including the diagnostic criteria) to determine whether the presence of cow's milk allergy (CMA) or asthma is associated with the risk of contracting IBD (altogether 2380 matched controls). A diagnosis of CMA in infancy was associated with Crohn disease (odds ratio [OR] 1.92, confidence interval [CI] 1.09-3.36, P
PubMed ID
23319082 View in PubMed
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Effect of CD8+ T-cell depletion on bronchial hyper-responsiveness and inflammation in sensitized and allergen-exposed Brown-Norway rats.

https://arctichealth.org/en/permalink/ahliterature15670
Source
Immunology. 1999 Mar;96(3):416-23
Publication Type
Article
Date
Mar-1999
Author
T J Huang
P A MacAry
D M Kemeny
K F Chung
Author Affiliation
Thoracic Medicine, Chang Gung Memorial Hospital, Keelung Branch, Taiwan, China; Thoracic Medicine, National Heart & Lung Institute, Imperial College School of Medicine, London, UK.
Source
Immunology. 1999 Mar;96(3):416-23
Date
Mar-1999
Language
English
Publication Type
Article
Keywords
Acetylcholine - immunology
Allergens - immunology
Animals
Antibodies, Monoclonal - immunology
Asthma - immunology - physiopathology
Blotting, Southern
Bronchial Hyperreactivity - immunology
Bronchoalveolar Lavage Fluid - immunology
CD8-Positive T-Lymphocytes - immunology
Cytokines - biosynthesis
Immunoenzyme Techniques
Lung - immunology
Lymphocyte Count
Male
Mice
Ovalbumin - immunology
Rats
Rats, Inbred BN
Reverse Transcriptase Polymerase Chain Reaction
Vasodilator Agents - immunology
Abstract
We examined the role of CD8+ T cells in a Brown-Norway rat model of asthma, using a monoclonal antibody to deplete CD8+ T cells. Ovalbumin (OA)-sensitized animals were given anti-CD8 antibody (0.5 mg/rat) intravenously 1 week prior to exposure to 1% OA aerosol and were studied 18-24 hr after aerosol exposure. Following administration of anti-CD8 antibody, CD8+ cells were reduced to
PubMed ID
10233723 View in PubMed
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[Effect of polarized light on the immune status and cytokine levels of patients with bronchial asthma during immunotherapy with bronchomunal]

https://arctichealth.org/en/permalink/ahliterature15354
Source
Fiziol Zh. 2002;48(3):87-94
Publication Type
Article
Date
2002
Author
O V Kravchenko
Author Affiliation
P.L. Shupik Academy of Post-Graduate Education, Ministry of Public Health of Ukraine.
Source
Fiziol Zh. 2002;48(3):87-94
Date
2002
Language
Ukrainian
Publication Type
Article
Keywords
Adjuvants, Immunologic - therapeutic use
Asthma - immunology - physiopathology - therapy
Combined Modality Therapy
Cytokines - blood
English Abstract
Humans
Immunoglobulin E - blood
Immunotherapy
Phototherapy
T-Lymphocyte Subsets - immunology
Treatment Outcome
Abstract
The influence of polarized polychromatic light on immunocompetent cells in complex with immunomodulated bronchomunal is studied. Data of content of the main cytokines taking part in development of inflammation are presented. It is cleared up that polarized light increases the number of T-lymphocyties, normalizes ratio of subpopulation of T-lymphocyties and level of serum FNO-alpha and level of interleukin-4 reaches the level of healthy people. It is ascertained that complex use bronchomunal and polarized polychromatic increases level of serum interferon-gamma.
PubMed ID
12125291 View in PubMed
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Endothelin-1 expression during early response after antigen challenge in brown-norway rats.

https://arctichealth.org/en/permalink/ahliterature15688
Source
Pulm Pharmacol Ther. 1998 Apr-Jun;11(2-3):215-9
Publication Type
Article
Author
M V Sánchez-Cifuentes
M L Rubio
M. Ortega
R. Largo
M D Gomez-Garre
N. Gonzalez Mangado
G. Peces-Barba
Author Affiliation
Pneumology Experimental Laboratory and Nephrology Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
Source
Pulm Pharmacol Ther. 1998 Apr-Jun;11(2-3):215-9
Language
English
Publication Type
Article
Keywords
Animals
Antigens - immunology
Asthma - immunology - physiopathology
Bronchoconstriction - immunology
Endothelin-1 - biosynthesis - genetics - immunology
Gene Expression
Hypersensitivity - immunology - physiopathology
Inhalation Exposure
Male
RNA, Messenger - biosynthesis - immunology
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Respiratory Function Tests
Reverse Transcriptase Polymerase Chain Reaction
Abstract
The objective of this study is to investigate if endothelin-1 (ET-1) gene expression changes during the early response phase following antigen challenge. We used sensitized Brown-Norway rats known to develop an early airway response after antigen challenge. After ovalbumin challenge, sensitized rats presented an early response, characterized by an increase in pulmonary pressure (209+/-14.53%,P
PubMed ID
9918759 View in PubMed
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Pulmonary function changes and increased Th-2 cytokine expression and nuclear factor kB activation in the lung after sensitization and allergen challenge in brown Norway rats.

https://arctichealth.org/en/permalink/ahliterature15559
Source
Immunol Lett. 2000 Jul 3;73(1):57-64
Publication Type
Article
Date
Jul-3-2000
Author
C C Lin
C Y Lin
H Y Ma
Author Affiliation
Chest Division and Department of Medical Research, Mackay Memorial Hospital, 92, Sec 2, Chung Shan North Road, Taipei, Taiwan, ROC.
Source
Immunol Lett. 2000 Jul 3;73(1):57-64
Date
Jul-3-2000
Language
English
Publication Type
Article
Keywords
Allergens - immunology
Animals
Asthma - immunology - physiopathology
Bronchial Hyperreactivity - immunology - physiopathology
Bronchial Provocation Tests
Bronchoalveolar Lavage Fluid - immunology
Cytokines - genetics - metabolism
Eosinophils - immunology
Lung - immunology - pathology - physiopathology
Male
Maximal Expiratory Flow Rate
NF-kappa B - immunology - metabolism
Ovalbumin - immunology
RNA, Messenger - biosynthesis
Rats
Research Support, Non-U.S. Gov't
Th2 Cells - metabolism
Abstract
BACKGROUND: Our purpose was to evaluate the expression of Th-1 and Th-2 related cytokine mRNA and nuclear factor (NF) kB in the lung tissue of ovalbumin (OA) sensitized brown Norway rats (BNR). We also evaluated the correlation between bronchial hyperreactivity (BHR) and eosinophils with cytokine mRNA expression. METHODS: Eight BNR (weight range 250-350 g) were sensitized by inhaled OA (group I) with a 1-week interval between and then provoked with OA 1 week later. Pulmonary function tests (PFT) were performed at baseline and 24 h after acetylcholine challenge. Eight weight-matched normal BNR served as controls (group II). All animals were anesthetized, paralyzed with gallamine, and ventilated via tracheostomy. They were given varying doses of acetylcholine (25, 50, 75, 100 microg/kg) injected through a jugular venous catheter. Five seconds after acetylcholine injections, PFTs were performed, including a maximal forced expiratory maneuver (MFEM), airway opening pressure (P(ao)) at tidal breathing and total dynamic lung compliance (C(dyn)). Bronchoalveolar lavage (BAL) was then performed with 20 ml normal saline divided into two doses. Thereafter, the lungs were removed and examined histologically. Total RNA was extracted from lung tissue samples and reverse-transcriptase polymerase chain reaction was performed using primers for mRNA of IL-4, IL-5, IL-10, interferon-gamma (IFNr) and beta-actine. RESULTS: Group I OA treated rats had typical airway obstruction on PFTs and airway inflammation on histological examination. Ratios of IL-4, IL-5, IL-10 and inducible nitric oxide synthase (iNOS) mRNA levels to beta-actine as measured by densitometry were significantly lower in controls than in OA-sensitized rats. The IFNr mRNA to beta-actin ratio was significantly reduced in OA-sensitized rats. Group I demonstrated a band shift when compared with group II in electromobility shift assay (EMSA) for NF-kB indicating increased activation of this transcription factor. CONCLUSION: Th-2 related cytokine mRNA was increased but Th-1 related cytokine mRNA was decreased in OA-sensitized BNR. An increased level of Th-2 related cytokine mRNA correlated with decreased airflow and inflammatory changes. These results demonstrate the value of the BNR model for studying allergic asthma at the molecular level.
PubMed ID
10963812 View in PubMed
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[The validation of the frequency of performing plasmapheresis in the treatment of bronchial asthma patients]

https://arctichealth.org/en/permalink/ahliterature15696
Source
Lik Sprava. 1998 Aug;(6):108-12
Publication Type
Article
Date
Aug-1998
Author
M P Zakharash
M M Kovalenko
L S Kogosova
Iu Ia Tkachenko
A D Chopchyk
I O Panashchuk
Source
Lik Sprava. 1998 Aug;(6):108-12
Date
Aug-1998
Language
Ukrainian
Publication Type
Article
Keywords
Adrenal Cortex Hormones - therapeutic use
Antibody formation
Asthma - immunology - physiopathology - therapy
Bronchodilator Agents - therapeutic use
Combined Modality Therapy
Comparative Study
English Abstract
Humans
Immunity, Cellular
Plasmapheresis - methods
Respiratory Function Tests - statistics & numerical data
Time Factors
Abstract
On the basis of the analysis of plasmapheresis (PPh) influence on clinical and functional and immunological indices in 105 patients with bronchial asthma the authors have come to the conclusion that PPh effect is the number-of-procedures-dependent. The most pronounced effect occurred in those groups having been given three to five PPh procedures. The above effect begins to reveal itself during the first postoperative week but the greatest positive changes take place between the 7th and the 14th days. As it won't make any difference whether three or five PPh sessions are conducted, the authors recommend plasmapheresis to be done on a three-procedure basis.
PubMed ID
9844891 View in PubMed
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7 records – page 1 of 1.