The house dust mite, Dermatophagoides farinae, was fractionated by a Sephadex G-200 column. Its allergenic (IgE-reacting) and immunogenic (IgG-reacting) components were investigated. By means of skin test, the molecular weight (MW) of major allergenic components of mite was found to be approximately 9,000 to 21,000 daltons. Immunogenic components were investigated by enzyme linked immunosorbent assay using each fraction as an antigen and mice plasma and human serum as antibodies. With mouse plasma, high IgG antibody titers were observed in fractions that contained the part of the mite with high MW (greater than 150,000). With human sera, high IgG antibody titers were observed in fractions that contained the part of the mite with MW more than 30,000. Heterogeneity of human IgG antibody responses against mite antigen was also suggested.
[Antigenic anachronism of the influenza A(H2N2) viruses in Leningrad in 1980. I. The epidemiological and serological characteristics of the influenzal infection caused by A/Leningrad/80 (H2N2) viruses].
In April-May 1980 a number of unrelated outbreaks of influenza-like diseases were registered in Leningrad in an infant home (50 out of 68 children under observation, aged 3 months to 2 years, were affected) and among the pupils of a boarding school (13 out of 50 adolescents under observation, aged 15-17 years, were affected). 5 strains of influenza A virus were isolated from 3 sick children and 1 clinically healthy child. A similar virus was isolated from a sick adolescent in a boarding school, as well as from a female patient aged 24 years at a domiciliary focus of infection (a sporadic case). In the subsequent laboratory investigation all these 7 strains were identified as viruses A/H2N2. Isolated cases of seroconversion to hemagglutinin H2 were definitely registered in 6 patients during February--May 1980. In 3 cases, including the 24-year old female patient with an acute respiratory disease, seroconversion to hemagglutinin H2 was observed in combination with the release of influenza viruses A/H2N2 from these patients. 2 influenza virus strains with this antigenic characteristic were isolated from a young female patient at an interval of 3 days. Among the patients admitted to the clinics of the Research Institute of Influenza in Leningrad on account of acute respiratory diseases isolated cases of diagnostically significant seroconversion to hemagglutinin H2 constituted 3.5% among children and 4.5% among adults. The study of the level of antihemagglutinins in the population revealed that in 1980 persons aged 18-50 years showed a high level of antihemagglutinins.(ABSTRACT TRUNCATED AT 250 WORDS)
The possibility of practical use of an increase in the level of antibody to a future causative agent of epidemic from minimal to the "critical" level in the human population against the background of a relative epidemic-free state in a town as a prepotent of an epidemic increase of influenza incidence was studied. During 4 spring and 3 autumn seasons (1978--1981) titres of antihemagglutinins to influenza A (H3N2) and B viruses were determined in 200--250 blood serum specimens from residents of Moscow selected with similar age distribution for each season against a working reference human serum. From the analysis of the antibody dynamics influenza A (H3N2) epidemic in 1979 and influenza B epidemic in 1980 were timely prognosed.
BACKGROUND: Although living kidney donors are increasingly being used, in most centers, 30 to 40 percent of potential donors are being turned down due to ABO mismatch. A protocol for ABO-mismatched kidney transplantation without splenectomy and with antigen-specific adsorption of ABO antibodies instead of nonspecific plasmapheresis was therefore designed. STUDY DESIGN AND METHODS: The immunosuppressive protocol used at the Department of Transplantation Surgery, Karolinska University Hospital, Stockholm, Sweden, together with relevant clinical data for 11 of the studied patients, have been described previously. The protocol called for immunoadsorption of ABO antibodies on Days -6, -5, -2, and -1 and on Days +2, +5, and +8. Patient plasma was recirculated through a new apheresis filter, the Glycosorb ABO column (Glycorex Transplantation AB), containing synthetic terminal trisaccharide A or B blood group antigen linked to a Sepharose matrix. RESULTS: Since 2001, 15 patients, including 2 infants, have been successfully transplanted with ABO-mismatched kidneys from living donors. The donor-recipient blood groups were A1-O (n = 5), A2-O (n = 2), B-O (n = 4), B-A (n = 2), and A1B-B (n = 2). ABO antibody titers at transplantation did not exceed immunoglobulin G 4 or immunoglobulin M 4 levels. No humoral rejections and no late rebound of ABO antibodies were observed. No adverse effects related to immunoadsorption treatment have been recorded. CONCLUSION: It is concluded that ABO-mismatched kidney transplantations can be successfully performed without splenectomy and that ABO antibodies can be effectively and safely depleted with the Glycosorb ABO column.
The time course of population immunity to influenza viruses A and B in different social and age groups among the inhabitants of Severodvinsk before and after the introduction of the complex system of influenza control has been studied. The influence of mass vaccinal prophylaxis and natural influenza morbidity on population immunity among the inhabitants of the city is shown.
To give a rationale for using homologous gamma globulin with antihemagglutinin titers of 1 : 20 to 1 : 80 for the prophylaxis of tick-borne encephalitis, 5-year observations covering all persons attacked by ticks in one of the intensive natural foci of the disease in the Western Urals have been made. The threefold statistically significant difference in the morbidity rate of tick-borne encephalitis between groups of persons immunized and not immunized with gamma globulin has been shown.
Four commercial enzyme immunoassays (EIAs) for the detection of parvovirus B19-specific immunoglobulin M (IgM) antibodies [Biotrin Parvovirus B19 IgM (Biotrin International, Ireland); Parvoscan B19 IgM (Euro-Diagnostica, Sweden); Parvovirus IgM (Immunobiological Laboratories [IBL], Germany); and human parvovirus B19 IgM (Hillcrest Biologicals, USA)] were compared to indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR). Using IFA as the reference test, high sensitivities (> or = 97%) were observed with all four EIAs, though the specificities of the Biotrin and IBL EIAs (99% and 96% respectively) were significantly higher than those of the Hillcrest and Euro-Diagnostica EIAs (81% and 79% respectively).
Gm phenotype frequencies were examined in 112 Swedish myasthenia gravis patients. The G1m 1,2,3 phenotype frequency in the total patient material did not differ significantly from that found in the normal population. However, when patients were subdivided, three different patient groups were observed with regard to Gm1 frequency: (1) Thymoma patients having a low frequency of Gm1, (2) Non-thymoma patients with a mild disease having a low frequency of Gm1 and (3) Non-thymoma patients with a severe disease having a high frequency of Gm1. When patients were subdivided according to presence or absence of HLA-B8 and Gm1 respectively, severe symptoms were less frequent in the HLA-B8+, Gm(-1) group as compared to the HLA-B8+, Gm(+1) group. Furthermore, there was an increased frequency of sera with anti-immunoglobulins not inhibitable by pooled control immunoglobulins.
Blood sera from 317 children with the history of noninfectious diseases or normal children pretreated with RDE were collected and examined for antihemagglutinins to 7 influenza virus strains in order to check the results of studies of 1980 and to study the immunity status to influenza A (H1N1), A (H2N2), A (H3N2) and B in the preepidemic period of 1981. Negative results of antihemagglutinin detection to influenza A/Iksha/1/57 (H2N2) in sera of all the children have confirmed the conclusion made in the previous paper (1) that influenza A (H1N2) viruses have not circulated in recent years among the population of Moscow City and suburban areas. Fourteen sera positive in HI tests with A/Shklyawer/49 virus similar to A/PR/8/34 (H1N1) in titres from 1:20 to 1:40 did not produce hemolysis zones with A/PR/8/34 virus in RHT, but most of these sera gave similar results of both tests with A/Brazil/11/79 and A/Khabarovsk/1/77 viruses. This attests to cross, nonspecific nature of hemagglutination-inhibition with A/Shklyawer/49 virus which evidently does not circulate now in the population of Moscow City and suburbs. An increase in the level of immunity to influenza B and A (H1N1)/1977 viruses reflected the epidemic situation of the previous year.
Immune complexes (IC) were detected and isolated from the serum of Brown Norway (BN), (Lewis x BN)F1, and Lewis rats bearing a Moloney sarcoma (MST). IC were isolated from the serum of individual rats employing a system of G-200 chromatography and passage through a heavy chain specific anti-rat IgG Immunoadsorbent. IgG and IgM were identified in the isolated IC by polyacrylamide gel electrophoresis (PAGE) and co-precipitation radioimmunoassays. Employing monospecific antibodies, IC consisting of IgG and IgM were bound to Raji cells as assessed by radioimmunoassay and indirect immunofluorescence. Raji binding activity of IC-containing serum was substantially reduced by pretreatment with dithiothreitol or incubation with anti-rat IgM or pooled normal rat IgG: F(ab')2. Sucrose density gradient ultracentrifugation under acid conditions dissociated IC into 7S IgG and 19S IgM components which recombined when co-incubated at pH 7 . 5. Viral antigens (gp70 and p30) were not detected in IC by PAGE and co-precipitation radioimmunoassay. Findings show that sera of rats bearing MST contain IC consisting predominantly of immunoglobulin. An IgM component which was separated, isolated and identified within IC containing serum displays anti-F(ab')2 reactivity.