Skip header and navigation

2 records – page 1 of 1.

Development of a peptide ELISA for the diagnosis of Aleutian mink disease.

https://arctichealth.org/en/permalink/ahliterature277369
Source
PLoS One. 2016;11(11):e0165793
Publication Type
Article
Date
2016
Author
Ma F
Zhang L
Wang Y
Lu R
Hu B
Lv S
Xue X
Li X
Ling M
Fan S
Zhang H
Yan X
Source
PLoS One. 2016;11(11):e0165793
Date
2016
Language
English
Publication Type
Article
Abstract
Aleutian disease (AD) is a common immunosuppressive disease in mink farms world-wide. Since the 1980s, counterimmunoelectrophoresis (CIEP) has been the main detection method for infection with the Aleutian Mink Disease Virus (AMDV). In this study, six peptides derived from the AMDV structural protein VP2 were designed, synthesized, and used as ELISA antigens to detect anti-AMDV antibodies in the sera of infected minks. Serum samples were collected from 764 minks in farms from five different provinces, and analyzed by both CIEP (a gold standard) and peptide ELISA. A peptide designated P1 (415 aa-433 aa) exhibited good antigenicity. A novel ELISA was developed using ovalbumin-linked peptide P1 to detect anti-AMDV antibodies in mink sera. The sensitivity and specificity of the peptide ELISA was 98.0% and 97.5%, respectively. Moreover, the ELISA also detected 342 early-stage infected samples (negative by CIEP and positive by PCR), of which 43.6% (149/342) were true positives. These results showed that the peptide ELISA had better sensitivity compared with CIEP, and therefore could be preferable over CIEP for detecting anti-AMDV antibodies in serological screening.
PubMed ID
27802320 View in PubMed
Less detail

Performance of AEP Monitor/2-derived composite index as an indicator for depth of sedation with midazolam and alfentanil during gastrointestinal endoscopy.

https://arctichealth.org/en/permalink/ahliterature80133
Source
Eur J Anaesthesiol. 2007 Mar;24(3):252-7
Publication Type
Article
Date
Mar-2007
Author
Huang Y-Y
Chu Y-C
Chang K-Y
Wang Y-C
Chan K-H
Tsou M-Y
Author Affiliation
National Yang-Ming University, Taipei Veterans General Hospital & School of Medicine, Department of Anaesthesiology, Taiwan.
Source
Eur J Anaesthesiol. 2007 Mar;24(3):252-7
Date
Mar-2007
Language
English
Publication Type
Article
Keywords
Adult
Alfentanil - pharmacology
Anesthetics, Intravenous - pharmacology
Blood Pressure - drug effects
Conscious Sedation - methods
Endoscopy, Gastrointestinal - methods
Evoked Potentials, Auditory - physiology
Female
Heart Rate - drug effects
Humans
Male
Midazolam - pharmacology
Middle Aged
Monitoring, Intraoperative - instrumentation - methods
Oxygen - blood
Prospective Studies
ROC Curve
Reproducibility of Results
Sensitivity and specificity
Abstract
BACKGROUND AND OBJECTIVE: The A-Line auditory evoked potential index (AAI) (AEP Monitor/2, Danmeter A/S, Odense, Denmark) is a newly developed composite parameter representing the degree of hypnosis. We conducted a prospective, observational study to explore the performance and validity of the AAI during conventional sedation for gastrointestinal (GI) endoscopy. METHODS: Thirty adults of either sex, age
PubMed ID
17054809 View in PubMed
Less detail