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[Analysis of complete sequence of cryptic plasmid pTP33 from Yersinia pestis isolated in Tuva natural focus of plague].

https://arctichealth.org/en/permalink/ahliterature289526
Source
Genetika. 2016 Sep; 52(9):1012-20
Publication Type
Journal Article
Date
Sep-2016
Author
M V Afanas’ev
S V Balakhonov
E G Tokmakova
V S Polovinkina
E A Sidorova
V V Sinkov
Source
Genetika. 2016 Sep; 52(9):1012-20
Date
Sep-2016
Language
Russian
Publication Type
Journal Article
Keywords
Bacterial Proteins - genetics
Plague - genetics
Plasmids - genetics
Siberia
Yersinia pestis - genetics - isolation & purification
Abstract
This paper studies a full nucleotide sequence of cryptic plasmid pTP33, which was isolated from the typical plague strain of the Tuvinian natural focus, Yersinia pestis I-2638. Sequencing was carried out using the 454 GS Junior platform (Roche). In analysis using the software package GS De Novo Assembler v. 2.7 (Roche) and the algorithm Newbler v. 2.7, 1855 nucleotide reads, which contained 1101246 nucleotides, were assembled to a contig of 33 978 bp. The GC content of the obtained nucleotide sequence was 50.25%. During annotation, we found 56 open reading frames. Homologs of the predicted reading frames were sought in the BLAST databases. We detected 22 reading frames coding hypothetical proteins, 23 frames coding phagerelated proteins, and 11 frames coding proteins with known functions, including toxin–antitoxin system YefM-YoeB, nucleic acids and polysaccharides metabolism proteins (exopolysaccharide production protein ExoZ, exodeoxyribonuclease VIII), and replication proteins (ParA). Some predicted pTP33 proteins were found to be homologs (from 45 to 75%) with sequences of phage-related proteins of certain microorganisms—endosymbionts of insects (Sodalis glossinidius) and endosymbionts of entomopathogenic nematodes (Photorhabdus luminescens, P. asymbiotica, Xenorhabdus bovienii).
PubMed ID
29369556 View in PubMed
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[Analysis of complete sequence of cryptic plasmid pTP33 from Yersinia pestis isolated in Tuva natural focus of plague].

https://arctichealth.org/en/permalink/ahliterature289684
Source
Genetika. 2016 Sep; 52(9):1012-20
Publication Type
Journal Article
Date
Sep-2016
Author
M V Afanas’ev
S V Balakhonov
E G Tokmakova
V S Polovinkina
E A Sidorova
V V Sinkov
Source
Genetika. 2016 Sep; 52(9):1012-20
Date
Sep-2016
Language
Russian
Publication Type
Journal Article
Keywords
Bacterial Proteins - genetics
Plague - genetics
Plasmids - genetics
Siberia
Yersinia pestis - genetics - isolation & purification
Abstract
This paper studies a full nucleotide sequence of cryptic plasmid pTP33, which was isolated from the typical plague strain of the Tuvinian natural focus, Yersinia pestis I-2638. Sequencing was carried out using the 454 GS Junior platform (Roche). In analysis using the software package GS De Novo Assembler v. 2.7 (Roche) and the algorithm Newbler v. 2.7, 1855 nucleotide reads, which contained 1101246 nucleotides, were assembled to a contig of 33 978 bp. The GC content of the obtained nucleotide sequence was 50.25%. During annotation, we found 56 open reading frames. Homologs of the predicted reading frames were sought in the BLAST databases. We detected 22 reading frames coding hypothetical proteins, 23 frames coding phagerelated proteins, and 11 frames coding proteins with known functions, including toxin–antitoxin system YefM-YoeB, nucleic acids and polysaccharides metabolism proteins (exopolysaccharide production protein ExoZ, exodeoxyribonuclease VIII), and replication proteins (ParA). Some predicted pTP33 proteins were found to be homologs (from 45 to 75%) with sequences of phage-related proteins of certain microorganisms—endosymbionts of insects (Sodalis glossinidius) and endosymbionts of entomopathogenic nematodes (Photorhabdus luminescens, P. asymbiotica, Xenorhabdus bovienii).
PubMed ID
29369556 View in PubMed
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[Change in the habitat of Yersinia pestis in the Gorno-Altaisk natural focus of plague].

https://arctichealth.org/en/permalink/ahliterature262345
Source
Med Parazitol (Mosk). 2014 Oct-Dec;(4):11-9
Publication Type
Article
Author
V M Korzun
E V Chipanin
S V Balakhonov
A V Denisov
E N Rozhdestvenskii
E P Mihailov
M B Iarygina
S A Kosilko
Source
Med Parazitol (Mosk). 2014 Oct-Dec;(4):11-9
Language
Russian
Publication Type
Article
Keywords
Animals
Disease Reservoirs
Ecosystem
Humans
Insect Vectors
Mammals - microbiology
Mongolia
Plague - epidemiology - transmission
Russia
Yersinia pestis - pathogenicity
Abstract
The paper analyzes the change that occurred in the habitat of the causative agent of plague in its Gorno-Altaisk natural focus in 1961 to 2012. Since 1961 when the plague microbe was found to come from the southern slopes of the Saylyugem mountain range, which are located in Mongolia, to the northern slopes situated in Russia, a gradual expansion of the habitat of Yersenia pestis subsp. altaica had commenced in South-Eastern Altai. During the considered period, the area where epizootic manifestations were registered showed an 11-fold increase. In most cases, the spread of the plague pathogen within the focus was natural and occurred in the successive and closely related settlements of Mongolian pikas (Ochotona pallasi). By now, the plague microbe has been widely distributed in three populations of this small animal, which inhabit the territory of South-Eastern Altai.
PubMed ID
25812401 View in PubMed
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Chitin and Products of Its Hydrolysis in Vibrio cholerae Ecology.

https://arctichealth.org/en/permalink/ahliterature267675
Source
Biochemistry (Mosc). 2015 Sep;80(9):1109-16
Publication Type
Article
Date
Sep-2015
Author
E Yu Markov
E S Kulikalova
L Ya Urbanovich
V S Vishnyakov
S V Balakhonov
Source
Biochemistry (Mosc). 2015 Sep;80(9):1109-16
Date
Sep-2015
Language
English
Publication Type
Article
Abstract
The role of chitin and its hydrolysis products generated by Vibrio cholerae chitinases in mechanisms of its adaptation in water environments, metabolism, preservation, acquisition of pathogenic potential, and its epidemiological value are reviewed. Chitin utilization by V. cholerae as a source of energy, carbon, and nitrogen is described. Chitin association promotes biofilm formation on natural chitinous surfaces, increasing V. cholerae resistance to adverse factors in ecological niches: the human body and water environments with its inhabitants. Hydrolytic enzymes regulated by the corresponding genes result in complete chitin biodegradation by a chitinolytic catabolic cascade. Consequences of V. cholerae cell and chitin interaction at different hierarchical levels include metabolic and physiological cell reactions such as chemotaxis, cell division, biofilm formation, induction of genetic competence, and commensalic and symbiotic mutual relations with higher organisms, nutrient cycle, pathogenicity for humans, and water organisms that is an example of successful interrelation of bacteria and substratum in the ecology of the microorganism.
PubMed ID
26555464 View in PubMed
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[Detection of "hybrid" Vibrio cholerae eltor strains during epidemic complications in Syberia and Far East].

https://arctichealth.org/en/permalink/ahliterature129079
Source
Zh Mikrobiol Epidemiol Immunobiol. 2011 Sep-Oct;(5):12-8
Publication Type
Article
Author
L V Mironova
S V Balakhonov
L Ia Urbanovich
V S Polovinkina
A S Kozhevnikova
E S Kulikalova
M V Afanas'ev
Source
Zh Mikrobiol Epidemiol Immunobiol. 2011 Sep-Oct;(5):12-8
Language
Russian
Publication Type
Article
Keywords
Amino Acid Sequence
Bacterial Typing Techniques
Cholera - epidemiology - microbiology
Cholera Toxin - genetics
Disease Outbreaks
Far East
Genes, Bacterial
Genotype
Humans
Molecular Sequence Data
Siberia
Vibrio cholerae - genetics - isolation & purification
Virulence - genetics
Abstract
Biotyping of Vibrio cholerae eltor isolated during epidemic complications of cholera in Syberia and Far East by phenotypic and genotypic properties complex.
45 strains of V. cholerae were studied. Phenotypic analysis was performed by using a complex ofbiovar determining tests. Genotyping was performed by detecting ctxAB, tcpA, toxR, rstRgenes, and ctxB gene structure analysis.
All the V. cholerae during epidemiologic complications in Syberia in the 1970s belong to eltor biovar by phenotypic properties and have eltor specific alleles of tcpA and rstR genes, and ctxB of the third genotype in the genome. In the 1990s the strains were phenotypically matching eltor biovar, but had genetical determinants of both eltor(tcpAE1, rstRE1) and classical (ctxB1, rstR(Cl) biovar.
The cause of epidemic complications of cholera in Syberia in the 1970s was V. cholerae eltor with typical eltor biovarphenotypical and genotypical properties. In the 1990s cases of introduction into the region of "hybrid: V. cholerae eltor strain were ascertained, developing into acute cholera outbreaks in several cases.
PubMed ID
22145342 View in PubMed
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Effect of Metal-Containing Nanocomposites on Functional Status of the Thymus in Experimental Animals.

https://arctichealth.org/en/permalink/ahliterature281458
Source
Bull Exp Biol Med. 2017 Mar;162(5):666-670
Publication Type
Article
Date
Mar-2017
Author
V I Dubrovina
S V Balakhonov
V V Voitkova
S A Vityazeva
T P Starovoitova
K M Korytov
G F Prozorova
G P Aleksandrova
S I Kolesnikov
Source
Bull Exp Biol Med. 2017 Mar;162(5):666-670
Date
Mar-2017
Language
English
Publication Type
Article
Abstract
The effects of nanostructurized composites argentogalactomannan and argento-poly-1-vinyl-1,2,4-triasole on the kinetics of T cell maturation in the thymus of albino mice were studied. These nanocomposites stimulated the formation of CD3(+)CD4(+)CD8(-) and CD3(+)CD4(-)CD8(+) cells from CD3(-)CD4(-)CD8(-) cells, the effect of argentogalactomannan was more potent. The efficiency of these compounds as agents improving the defense potential deserves special studies.
PubMed ID
28361422 View in PubMed
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Effect of Synthetic Organoselenium Drug on the Degree of Pathological Changes in the Organs of White Mice Immunized with Tularemia and Brucellosis Vaccines.

https://arctichealth.org/en/permalink/ahliterature308046
Source
Bull Exp Biol Med. 2019 Nov; 168(1):66-68
Publication Type
Journal Article
Date
Nov-2019
Author
O V Yurieva
V I Dubrovina
V A Potapov
M V Musalov
T P Starovoitova
T A Ivanova
A V Gromova
T T Shkaruba
S V Balakhonov
Author Affiliation
Irkutsk Research Anti-Plague Institute, Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Irkutsk, Russia.
Source
Bull Exp Biol Med. 2019 Nov; 168(1):66-68
Date
Nov-2019
Language
English
Publication Type
Journal Article
Keywords
Animals
Bacterial Vaccines - immunology
Brucellosis - immunology
Magnetic Resonance Spectroscopy
Mice
Organoselenium Compounds - pharmacology
Synthetic Drugs - pharmacology
Tularemia - immunology
Abstract
We studied the effect of the organoselenium compound 2,6-dipyridinium-9-selenium-bicyclo[ 3,3,1]nonan dibromide (974zh) on the severity of pathological changes in the organs of experimental animals immunized with live tularemia and brucellosis vaccines. It was found that 974zh reduced reactogenicity of vaccines for experimental animals. Our findings indicate the prospects for further studies of the effects of 974zh on the functional state of experimental animals.
PubMed ID
31754926 View in PubMed
Less detail

Effects of Thermoextracts of Brucella S and L Forms on Lipid Peroxidation and Antioxidant Defense in Organs of Laboratory Animals.

https://arctichealth.org/en/permalink/ahliterature292426
Source
Bull Exp Biol Med. 2018 Jun 19; :
Publication Type
Journal Article
Date
Jun-19-2018
Author
V I Dubrovina
S V Balakhonov
O V Yurieva
T P Starovoitova
N L Barannikova
L E Tokareva
A B Pyatidesyatnikova
K Yu Yastremskaya
T T Shkaruba
Author Affiliation
Irkutsk Antiplague Research Institute of Siberia and Far East, Irkutsk, Russia. dubrovina-valya@mail.ru.
Source
Bull Exp Biol Med. 2018 Jun 19; :
Date
Jun-19-2018
Language
English
Publication Type
Journal Article
Abstract
The dynamics of LPO marker malondialdehyde formation and peroxidase-destroying activity was studied in homogenized organs of guinea pigs, immunized with thermoextracts from S and L forms Brucella abortus I-206. The L form brucella thermoextract exhibited a lower reactogenicity and adequately activated the antioxidant system, due to which the destructive effects of ROS could be partially neutralized during the vaccinal process.
PubMed ID
29923000 View in PubMed
Less detail

Effects of Thermoextracts of Brucella S and L Forms on Lipid Peroxidation and Antioxidant Defense in Organs of Laboratory Animals.

https://arctichealth.org/en/permalink/ahliterature296008
Source
Bull Exp Biol Med. 2018 Jun; 165(2):239-242
Publication Type
Journal Article
Date
Jun-2018
Author
V I Dubrovina
S V Balakhonov
O V Yurieva
T P Starovoitova
N L Barannikova
L E Tokareva
A B Pyatidesyatnikova
K Yu Yastremskaya
T T Shkaruba
Author Affiliation
Irkutsk Antiplague Research Institute of Siberia and Far East, Irkutsk, Russia. dubrovina-valya@mail.ru.
Source
Bull Exp Biol Med. 2018 Jun; 165(2):239-242
Date
Jun-2018
Language
English
Publication Type
Journal Article
Keywords
Animal Structures - drug effects - metabolism
Animals
Animals, Laboratory
Antioxidants - metabolism
Brucella Vaccine - chemistry - pharmacology
Brucella abortus - chemistry - immunology - pathogenicity
Female
Guinea Pigs
L Forms - physiology
Lipid Peroxidation - drug effects
Liver - drug effects - metabolism - pathology
Male
Oxidative Stress - drug effects
Spheroplasts - physiology
Temperature
Vaccines, Attenuated - chemistry - pharmacology
Abstract
The dynamics of LPO marker malondialdehyde formation and peroxidase-destroying activity was studied in homogenized organs of guinea pigs, immunized with thermoextracts from S and L forms Brucella abortus I-206. The L form brucella thermoextract exhibited a lower reactogenicity and adequately activated the antioxidant system, due to which the destructive effects of ROS could be partially neutralized during the vaccinal process.
PubMed ID
29923000 View in PubMed
Less detail

[Epidemiological situation and prophylaxis of zoonotic and natural-focal infectious diseases in Siberia and the Far East].

https://arctichealth.org/en/permalink/ahliterature112722
Source
Zh Mikrobiol Epidemiol Immunobiol. 2013 Jan-Feb;(1):62-6
Publication Type
Article
Author
S V Balakhonov
M V Chesnokova
E I Andaev
S A Kosilko
N V Breneva
Source
Zh Mikrobiol Epidemiol Immunobiol. 2013 Jan-Feb;(1):62-6
Language
Russian
Publication Type
Article
Keywords
Animals
Epidemiological Monitoring
Female
Humans
Male
Rabies
Siberia
Tick-Borne Diseases - epidemiology - prevention & control - transmission
Zoonoses
Abstract
Analysis of zoonotic and natural-focal infectious disease morbidity in 2009 - 2011 in Siberia and the Far East is presented, and a complex of measures aimed at their prophylaxis is proposed. Analysis is carried out based on the data received by Reference Center of Monitoring of Natural-Focal Infection Causative Agents and Regional Center of Monitoring of I-II Pathogenicity Group Causative Agents at the Irkutsk Research Institute of Plague Control from departments and Centers of Hygiene and Epidemiology of Siberian, Far Eastern, 3 subjects of Urals Federal District and 5 Stations of Plague Control of Federal Service for Control in the Sphere of Protection of Consumers' Rights and Well-Being of Humans. In the morbidity structure in this region "tick-borne" infections were established to predominate--69.4%, among bacterial--yersiniosis dominates. Deterioration of epizootic situation on rabies is observed in the Republics of Tuva and Buryatia.
PubMed ID
23805654 View in PubMed
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17 records – page 1 of 2.