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[Properties of NAD-glycohydrolase of the nuclei of the liver cells of rats]

https://arctichealth.org/en/permalink/ahliterature13299
Source
Ukr Biokhim Zh. 1975 Jan-Feb;47(1):3-7
Publication Type
Article
Author
L V Pakyrbaéva
S I Shushevich
A H Khalmuradov
R V Chahovets'
Source
Ukr Biokhim Zh. 1975 Jan-Feb;47(1):3-7
Language
Ukrainian
Publication Type
Article
Keywords
Animals
Cell Nucleus - drug effects - enzymology
Edetic Acid - pharmacology
Egtazic Acid - pharmacology
English Abstract
Hydrogen-Ion Concentration
Isonicotinic Acids - pharmacology
Liver - drug effects - enzymology
Mercaptoethanol - pharmacology
N-Glycosyl Hydrolases - metabolism
NAD - metabolism
NAD+ Nucleosidase - metabolism
NADP - metabolism
Niacinamide - pharmacology
Nicotinamide Mononucleotide - metabolism
Nicotinic Acids - pharmacology
Rats
Temperature
Abstract
Certain properties of the rat liver cell nuclei NAD-glycohydrolase (EC 3.2.2.5) were investigated. It is established that its highest activity is at 37 degrees with activation energy equal to 9480 cal/M and with factor Q10 equal to 1.5. The enzyme pH optimum in 0.2 M tris acetate is equal to 6.5 and in 0.2 potassium phosphate - 7.5. It was shown that the enzyme manifests its strict specificity only with beta-NAD, and it hardly decomposes NADP without affecting NADH, NADPH and NMN. The apparent Km value of the enzyme with respect to NAD is established. Isonicotinic acid hydrazide, nicotinamide and to the less extent nicotinic acid inhibit the enzymatic activity of nuclei. EDTA, EGTA, p-CMB, mercaptoethanol do not cause any changes in the rat liver cells nuclei NADase activity.
PubMed ID
830 View in PubMed
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