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5-oxo-6,8,11,14-eicosatetraenoic acid induces the infiltration of granulocytes into human skin.

https://arctichealth.org/en/permalink/ahliterature15223
Source
J Allergy Clin Immunol. 2003 Oct;112(4):768-74
Publication Type
Article
Date
Oct-2003
Author
Shigeo Muro
Qutayba Hamid
Ronald Olivenstein
Rame Taha
Joshua Rokach
William S Powell
Author Affiliation
Department of Medicine, McGill University, Montreal, Quebec, Canada.
Source
J Allergy Clin Immunol. 2003 Oct;112(4):768-74
Date
Oct-2003
Language
English
Publication Type
Article
Keywords
Arachidonic Acids - pharmacology
Asthma - physiopathology
Case-Control Studies
Cell Movement - drug effects
Chemotactic Factors - pharmacology
Granulocytes - drug effects - pathology
Humans
Macrophages - pathology
Mast Cells - pathology
Neutrophils - pathology
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Skin - pathology
Time Factors
Abstract
BACKGROUND: 5-Oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE) is an arachidonic acid metabolite with potent in vitro chemoattractant effects on eosinophils and neutrophils. It has also been shown to induce pulmonary eosinophilia in Brown Norway rats, but it is not known whether it is active in human beings in vivo. OBJECTIVE: To determine whether 5-oxo-ETE can induce cellular infiltration in patients with atopic asthma and nonatopic control subjects after intradermal administration. METHODS: 5-Oxo-ETE was administered intradermally to 11 patients with atopic asthma and 10 nonatopic control subjects. Skin biopsy specimens were taken 6 or 24 hours later and examined by immunocytochemistry for cells expressing specific markers for eosinophils (major basic protein), neutrophils (elastase), macrophages (CD68), lymphocytes (CD3), and mast cells (tryptase). RESULTS: 5-Oxo-ETE (1.5 and 5 microg) elicited the infiltration of both eosinophils and neutrophils into the skin in both control and atopic asthmatic subjects. Increased numbers of eosinophils were observed at 6 and 24 hours after injection, whereas significantly elevated neutrophil numbers were present only after 24 hours. Eosinophils were >3 times higher in patients with atopic asthma compared with control subjects after injection of the highest dose of 5-oxo-ETE. Macrophage numbers were also elevated, but only at the highest dose of 5-oxo-ETE. No effects were observed on the numbers of either lymphocytes or mast cells. CONCLUSIONS: 5-Oxo-ETE elicits the infiltration of eosinophils and neutrophils into the skin of human beings in vivo after intradermal administration. Asthmatic subjects are more responsive to this substance than nonallergic control subjects. These results suggest that 5-oxo-ETE may be an important mediator of inflammation.
PubMed ID
14564360 View in PubMed
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Airway Remodeling in Allergen Challenged Brown Norway Rats: Distribution of Proteoglycans.

https://arctichealth.org/en/permalink/ahliterature14996
Source
Am J Physiol Lung Cell Mol Physiol. 2005 Dec 30;
Publication Type
Article
Date
Dec-30-2005
Author
Laura Pini
Chiara Torregiani
James G Martin
Qutayba Hamid
Mara S Ludwig
Author Affiliation
Department of Medicine, Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.
Source
Am J Physiol Lung Cell Mol Physiol. 2005 Dec 30;
Date
Dec-30-2005
Language
English
Publication Type
Article
Abstract
Proteoglycans (PG) have important effects on the mechanical properties of tissues, and the phenotype of various structural cells. Little is known about changes in PG deposition in the airways in animal models of asthma. We studied changes in PG in the airway wall of Brown Norway rats sensitized to ovalbumin (OA) and exposed to repeated OA challenge. Control (Sal) animals were sensitized and challenged with saline. After the 3rd challenge, animals were sacrificed and lungs fixed in formalin. Tissue sections were incubated with antibodies to the small, leucine-rich PG, decorin and biglycan, and collagen type I. Airways were classified according to basement membrane perimeter (Pbm) length (/=3mm). Decorin, biglycan and collagen type I, were increased in the airways of OA vs Sal rats. Remodeling was most prominent in central airways. The distribution of PG differed with respect to the subepithelial vs airway smooth muscle (ASM) vs adventitial layer. Whereas biglycan was readily detected within the ASM, decorin and collagen were detected outside the ASM, and especially in the adventitial layer. Differences in the distribution of these molecules within the layers of the airway wall may reflect their specific functional roles.
PubMed ID
16387756 View in PubMed
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CD8+ alphabeta T cells can mediate late airway responses and airway eosinophilia in rats.

https://arctichealth.org/en/permalink/ahliterature57391
Source
J Allergy Clin Immunol. 2004 Dec;114(6):1345-52
Publication Type
Article
Date
Dec-2004
Author
Susumu Isogai
Rame Taha
Meiyo Tamaoka
Yasuyuki Yoshizawa
Qutayba Hamid
James G Martin
Author Affiliation
Department of Medicine, McGill University, Montreal, Quebec, Canada.
Source
J Allergy Clin Immunol. 2004 Dec;114(6):1345-52
Date
Dec-2004
Language
English
Publication Type
Article
Keywords
Adoptive Transfer
Animals
Bronchoalveolar Lavage Fluid - immunology
CD8-Positive T-Lymphocytes - immunology
Cytokines - biosynthesis
Eosinophilia - etiology
Lung Diseases - etiology
Male
Ovalbumin - immunology
Rats
Rats, Inbred BN
Receptors, Antigen, T-Cell, alpha-beta - analysis
Research Support, Non-U.S. Gov't
Abstract
BACKGROUND: The function of CD8+ T-cell subsets in mediating late allergic responses is incompletely understood. OBJECTIVE: We sought to test the hypothesis that CD8+ alphabeta T cells are proinflammatory in the airways in vivo by using a well-characterized animal model and the technique of adoptive transfer. METHODS: Brown Norway rats were administered CD8 + alphabeta T cells (10 6 ) intraperitoneally purified from lymph node cells of either naive or ovalbumin (OVA)-sensitized rats and were challenged with aerosolized OVA 2 days later. Control rats were sensitized to 100 mug of OVA in Al(OH) 3 subcutaneously or sham sensitized to saline and were OVA challenged 2 weeks later. RESULTS: The OVA-sensitized and OVA-challenged group and the recipients of OVA-primed CD8+ alphabeta T cells had significant late airway responses calculated from lung resistance measured for an 8-hour period after challenge compared with the naive CD8 + alphabeta T cell-transferred group and the sham-sensitized control group. The number of eosinophils in bronchoalveolar lavage fluid increased in the OVA-sensitized group and the OVA-primed CD8+ alphabeta T-cell recipients compared with numbers in the naive CD8+ alphabeta T-cell recipients and the sham-sensitized control group. IL-4 and IL-5 cytokine mRNA expression in bronchoalveolar lavage fluid increased in the OVA-sensitized group and the OVA-primed CD8+ alphabeta T-cell recipients compared with that in the sham-sensitized group. CONCLUSION: We conclude that antigen-primed CD8 + alphabeta T cells might have a proinflammatory role in allergen-driven airway responses in the rat.
PubMed ID
15577833 View in PubMed
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CD8 positive T cells express IL-17 in patients with chronic obstructive pulmonary disease.

https://arctichealth.org/en/permalink/ahliterature135451
Source
Respir Res. 2011;12:43
Publication Type
Article
Date
2011
Author
Ying Chang
Jessica Nadigel
Nicholas Boulais
Jean Bourbeau
François Maltais
David H Eidelman
Qutayba Hamid
Author Affiliation
Meakins-Christie Laboratories and Respiratory Division, Department of Medicine McGill University, 3626 rue St, Urbain, Montreal, QC, H2X 2P2 Canada.
Source
Respir Res. 2011;12:43
Date
2011
Language
English
Publication Type
Article
Keywords
Adult
Biopsy
Bronchi - immunology
Bronchoscopy
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Case-Control Studies
Female
Humans
Immunohistochemistry
Immunophenotyping
Interleukin-17 - analysis - genetics
Lasers
Male
Microdissection - instrumentation
Middle Aged
Pulmonary Disease, Chronic Obstructive - diagnosis - genetics - immunology
Quebec
RNA, Messenger - analysis
Reverse Transcriptase Polymerase Chain Reaction
Severity of Illness Index
Abstract
Chronic obstructive pulmonary disease (COPD) is a progressive and irreversible chronic inflammatory disease of the lung. The nature of the immune reaction in COPD raises the possibility that IL-17 and related cytokines may contribute to this disorder. This study analyzed the expression of IL-17A and IL-17F as well as the phenotype of cells producing them in bronchial biopsies from COPD patients.
Bronchoscopic biopsies of the airway were obtained from 16 COPD subjects (GOLD stage 1-4) and 15 control subjects. Paraffin sections were used for the investigation of IL-17A and IL-17F expression in the airways by immunohistochemistry, and frozen sections were used for the immunofluorescence double staining of IL-17A or IL-17F paired with CD4 or CD8. In order to confirm the expression of IL-17A and IL-17F at the mRNA level, a quantitative RT-PCR was performed on the total mRNA extracted from entire section or CD8 positive cells selected by laser capture microdissection.
IL-17F immunoreactivity was significantly higher in the bronchial biopsies of COPD patients compared to control subjects (P
Notes
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PubMed ID
21477350 View in PubMed
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The effects of CD8+gammadelta T cells on late allergic airway responses and airway inflammation in rats.

https://arctichealth.org/en/permalink/ahliterature57418
Source
J Allergy Clin Immunol. 2003 Sep;112(3):547-55
Publication Type
Article
Date
Sep-2003
Author
Susumu Isogai
Alexandra Rubin
Karim Maghni
David Ramos-Barbon
Rame Taha
Yasuyuki Yoshizawa
Qutayba Hamid
James G Martin
Author Affiliation
Meakins Christie Laboratories, Department of Medicine, McGill University, 3623 St Urbain, Montreal, Quebec, Canada H2X 2P2.
Source
J Allergy Clin Immunol. 2003 Sep;112(3):547-55
Date
Sep-2003
Language
English
Publication Type
Article
Keywords
Adoptive Transfer
Allergens - administration & dosage
Animals
CD8-Positive T-Lymphocytes - immunology
Cytokines - biosynthesis - genetics
Eosinophilia - immunology
Immunoglobulin E - blood
Interferon Type II - biosynthesis - genetics
Interleukin-4 - biosynthesis - genetics
Male
Models, Immunological
Ovalbumin - administration & dosage - immunology
RNA, Messenger - genetics - metabolism
Rats
Rats, Inbred BN
Receptors, Antigen, T-Cell, gamma-delta - metabolism
Research Support, Non-U.S. Gov't
Respiratory Hypersensitivity - etiology - immunology
T-Lymphocyte Subsets - immunology
Abstract
BACKGROUND: Gamma-delta (gammadelta) T cells regulate immune responses to foreign protein at mucosal surfaces. Whether they can modify allergen-induced early (EAR) and late airway responses (LAR) is unknown. OBJECTIVE: We have tested the hypothesis that the CD8+ subtype of gammadelta T cells decreases allergen-induced LAR and airway eosinophilia in the rat. METHODS: Brown Norway rats were administered, intraperitoneally, 3.5 x 10(4) lymph node CD8+gammadelta T cells from naive or sensitized rats. The recipients were sensitized to ovalbumin (OVA) in Al(OH)(3) 3 days after cell transfer and challenged with aerosolized OVA 14 days later. Serum IgE was measured before allergen challenge. After challenge, lung resistance was monitored for 8 hours and then bronchoalveolar lavage (BAL) was analyzed for eosinophil major basic protein (MBP), IL-4, IL-5, IL-13, and IFN-gamma messenger RNA-expressing cells. RESULTS: gammadelta T cells from naive donors significantly decreased LAR in OVA-challenged sensitized rats, whereas MBP(+) eosinophils were decreased by both gammadelta T cells from naive and sensitized donors. EAR and serum IgE levels were unchanged. The expression of IL-4, IL-5, and IL-13 by BAL cells of gammadelta T cell recipients was attenuated compared with OVA-challenged controls. This was accompanied by an increase in the expression of IFN-gamma. CONCLUSIONS: Our results are consistent with a suppressive role of CD8+gammadelta T cells on allergic airway responses. However, only gammadelta T cells from naive donors inhibit LAR.
PubMed ID
13679814 View in PubMed
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The effects of IL-5 on airway physiology and inflammation in rats.

https://arctichealth.org/en/permalink/ahliterature15276
Source
J Allergy Clin Immunol. 2003 Mar;111(3):558-66
Publication Type
Article
Date
Mar-2003
Author
Sammy S Nag
Li Jing Xu
Qutayba Hamid
Paolo M Renzi
Author Affiliation
CHUM Research Center, Notre Dame Pavillion, University of Montreal, and the Meakins-Christie Laboratories, McGill University, Quebec, Canada.
Source
J Allergy Clin Immunol. 2003 Mar;111(3):558-66
Date
Mar-2003
Language
English
Publication Type
Article
Keywords
Animals
Blood Cells - pathology
Bronchoalveolar Lavage Fluid - cytology
Cell Differentiation - drug effects
Cytokines - genetics
Dose-Response Relationship, Drug
Eosinophils - pathology
Humans
Immunization
Immunoglobulin E - blood
Inflammation - physiopathology
Interleukin-5 - administration & dosage - pharmacology
Intubation, Intratracheal
Lung - metabolism
Lymphocyte Subsets - pathology
Male
Methacholine Chloride - pharmacology
Ovalbumin - immunology
RNA, Messenger - metabolism
Rats
Rats, Inbred BN
Recombinant Proteins - administration & dosage - pharmacology
Research Support, Non-U.S. Gov't
Respiratory System - drug effects - physiopathology
Respiratory Tract Diseases - physiopathology
Stem Cells - pathology
Abstract
BACKGROUND: There is evidence that the cytokine IL-5 is a prominent feature of airway inflammation in asthma. OBJECTIVE: The aim of this study was to determine whether exogenous IL-5 could cause changes in lung physiology, the early and late airway response after antigen challenge, and airway inflammation in rats that do not have a propensity to develop these changes after sensitization and challenge. METHOD AND RESULTS: Intratracheal administration of IL-5 to ovalbumin sensitized Brown Norway SSN rats increased the airway responsiveness to methacholine (AHR) 20 hours after administration of IL-5 at the same time as an increase in neutrophils occurred in the lung lavage. This effect was dose dependent and was not caused by endotoxin. Concurrent intratracheal administration of 50 ng of anti-IL-5 monoclonal antibody with 10 microg of recombinant human IL-5 decreased the AHR and neutrophil influx. Pretreatment with 3 microg of IL-5 had no effect on the early and late airway response or on AHR after ovalbumin challenge. However, IL-5 increased lung re-sistance 20 hours after antigen challenge. Although total lung cells and differential counts did not differ significantly 8 hours after antigen challenge, the blood lymphocyte CD4/CD8 ratio decreased in IL-5 pretreated rats (P
PubMed ID
12642837 View in PubMed
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IL-4, IL-5 and IFN-gamma mRNA expression in pulmonary lymphocytes in equine heaves.

https://arctichealth.org/en/permalink/ahliterature15211
Source
Vet Immunol Immunopathol. 2004 Jan;97(1-2):87-96
Publication Type
Article
Date
Jan-2004
Author
Marie-Eve Cordeau
Philippe Joubert
Oday Dewachi
Qutayba Hamid
Jean-Pierre Lavoie
Author Affiliation
Faculté de Médecine Vétérinaire, Université de Montréal, 3200 Sicotte, St-Hyacinthe, Que., Canada J2S 7C6.
Source
Vet Immunol Immunopathol. 2004 Jan;97(1-2):87-96
Date
Jan-2004
Language
English
Publication Type
Article
Keywords
Animals
Bronchoalveolar Lavage Fluid - cytology - immunology
Female
Horse Diseases - genetics - immunology - metabolism
Horses
In Situ Hybridization - veterinary
Interferon Type II - biosynthesis - genetics - immunology
Interleukin-4 - biosynthesis - genetics - immunology
Interleukin-5 - biosynthesis - genetics - immunology
Lung Diseases, Obstructive - genetics - immunology - metabolism - veterinary
Male
RNA, Messenger - biosynthesis - blood - genetics
Research Support, Non-U.S. Gov't
Respiratory Function Tests - veterinary
Respiratory Hypersensitivity - genetics - immunology - metabolism - veterinary
Statistics, nonparametric
Abstract
Heaves is a common condition of horses of cold climate that is characterized by small airway inflammation and obstruction following exposure of susceptible horses to moldy hay and straw. It has been shown that helper T lymphocytes (Th) orchestrate the inflammatory response in asthma and in various animal models of allergic lung diseases by the release of Th2-type cytokines. Results of previous studies indicate that a predominant expression of Th2-type response by airway cells may also be present in heaves. To evaluate the temporal mRNA expression of Th1 (IFN-gamma) and Th2 (IL-4, IL-5) type cytokines in heaves and their relationship to clinical disease, we studied the pulmonary mechanics and cytokine mRNA expression (IL-4, IL-5 and IFN-gamma) in bronchoalveolar lavage lymphocytes of horses with heaves (n=6) and control (n=6) before and after 24h and 9 days of continuous natural inhalation challenge. Starting 24h after challenge horses with heaves, but not control horses, had a significant increase in pulmonary elastance and the number of lymphocytes expressing mRNA for IL-4 and IL-5. These changes were further increased at 9 days, at which time the number of cells positive for IFN-gamma mRNA was decreased. In this study we have shown that BAL lymphocytes of horses with heaves during clinical exacerbation have a predominant Th2-type cytokine response and that this response coincides in time with the presence of airway obstruction.
PubMed ID
14700540 View in PubMed
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Resident CD8+ T cells suppress CD4+ T cell-dependent late allergic airway responses.

https://arctichealth.org/en/permalink/ahliterature57383
Source
J Allergy Clin Immunol. 2005 Mar;115(3):521-6
Publication Type
Article
Date
Mar-2005
Author
Susumu Isogai
Sean Jedrzkiewicz
Rame Taha
Qutayba Hamid
James G Martin
Author Affiliation
Meakins Christie Laboratories, Department of Medicine, McGill University, Montreal, Quebec, Canada.
Source
J Allergy Clin Immunol. 2005 Mar;115(3):521-6
Date
Mar-2005
Language
English
Publication Type
Article
Keywords
Adoptive Transfer
Animals
Bronchoalveolar Lavage Fluid - cytology - immunology
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Flow Cytometry
Immunohistochemistry
In Situ Hybridization
Interferon Type II - immunology
Interleukin-4 - immunology
Interleukin-5 - immunology
Ovalbumin - immunology
RNA, Messenger
Rats
Research Support, Non-U.S. Gov't
Respiratory Hypersensitivity - immunology
Abstract
BACKGROUND: The role of CD8+ T cells in the immune response to airway challenge with an allergen is poorly understood. OBJECTIVE: The aim of this study was to test the hypothesis that resident naive CD8+ T cells modulate the magnitude of CD4+ T cell-dependent allergic airway responses. METHODS: Cervical lymph node CD4+ T cells (2 x 10(6)) were harvested from ovalbumin (OVA)- or sham-sensitized rats and injected intraperitoneally into naive Brown Norway recipients. The recipients were treated with a CD8alpha mAb (OX-8) to deplete the resident CD8+ T cells (n = 12) or mouse ascites (n = 12). Two days after adoptive transfer, the recipient animals were OVA challenged, lung resistance was measured for 8 hours, and bronchoalveolar lavage (BAL) was performed. RESULTS: After OVA challenge, primed CD4-transferred CD8-depleted rats had larger early airway responses and late airway responses compared with primed CD4-transferred CD8-nondepleted rats (early airway responses: 158.6% +/- 19.2% vs 115.7% +/- 5.9%, P
PubMed ID
15753899 View in PubMed
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8 records – page 1 of 1.