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Physiological characterization of the emergence from diapause: A transcriptomics approach.

https://arctichealth.org/en/permalink/ahliterature294500
Source
Sci Rep. 2018 Aug 22; 8(1):12577
Publication Type
Journal Article
Date
Aug-22-2018
Author
Vittoria Roncalli
Stephanie A Sommer
Matthew C Cieslak
Cheryl Clarke
Russell R Hopcroft
Petra H Lenz
Author Affiliation
Pacific Biosciences Research Center, University of Hawai'i at Manoa, 1993 East-West Rd., Honolulu, HI, 96822, USA. roncalli@hawaii.edu.
Source
Sci Rep. 2018 Aug 22; 8(1):12577
Date
Aug-22-2018
Language
English
Publication Type
Journal Article
Abstract
Organisms inhabiting high-latitude environments have evolved adaptations, such as diapause to time reproduction and growth to optimize their survival. However, the physiological regulation of the timing of complex life histories is poorly understood, particularly for marine copepods, that diapause at depth. A member of the pelagic community of the sub-Arctic Pacific Ocean, Neocalanus flemingeri enters diapause in June. Egg production occurs in winter/spring. In order to characterize the transition from diapause to egg release, females were collected in late September from 400-700?m depth, incubated in the dark at 4-5?°C and sampled for RNASeq at weekly intervals. The diapause phenotype showed down-regulation of protein turnover and up-regulation of stress genes. Activation of the reproductive program was marked by the up-regulation of genes involved in germline development. Thereafter, progress through phases of oocyte development could be linked to changes in gene expression. At 5 weeks, females showed up-regulation of spermatogenesis, indicating that stored sperm had been in a quiescent stage and completed their maturation inside the female. Gene expression profiles provide a framework to stage field-collected females. The 7-week progression from diapause to late oogenesis suggests that females typically spawning in January initiated the reproductive program in November.
Notes
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PubMed ID
30135598 View in PubMed
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Regional heterogeneity impacts gene expression in the subarctic zooplankter Neocalanus flemingeri in the northern Gulf of Alaska.

https://arctichealth.org/en/permalink/ahliterature309174
Source
Commun Biol. 2019; 2:324
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Date
2019
Author
Vittoria Roncalli
Matthew C Cieslak
Martina Germano
Russell R Hopcroft
Petra H Lenz
Author Affiliation
1Pacific Biosciences Research Center, University of Hawai'i at Manoa, 1993 East-West Rd., Honolulu, HI 96822 USA.
Source
Commun Biol. 2019; 2:324
Date
2019
Language
English
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Keywords
Alaska
Animals
Arctic Regions
Chlorophyll A - metabolism
Cluster analysis
Copepoda - genetics
Gene Expression Regulation
Gene Ontology
Lipid Metabolism - genetics
RNA, Messenger - genetics - metabolism
Salinity
Temperature
Zooplankton - genetics
Abstract
Marine pelagic species are being increasingly challenged by environmental change. Their ability to persist will depend on their capacity for physiological acclimatization. Little is known about limits of physiological plasticity in key species at the base of the food web. Here we investigate the capacity for acclimatization in the copepod Neocalanus flemingeri, which inhabits the Gulf of Alaska, a heterogeneous and highly seasonal environment. RNA-Seq analysis of field-collected pre-adults identified large regional differences in expression of genes involved in metabolic and developmental processes and response to stressors. We found that lipid synthesis genes were up-regulated in individuals from Prince William Sound and down-regulated in the Gulf of Alaska. Up-regulation of lipid catabolic genes in offshore individuals suggests they are experiencing nutritional deficits. The expression differences demonstrate physiological plasticity in response to a steep gradient in food availability. Our transcriptional analysis reveals mechanisms of acclimatization that likely contribute to the observed resilience of this population.
PubMed ID
31482143 View in PubMed
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