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Repeated allergen exposure of sensitized Brown-Norway rats induces airway cell DNA synthesis and remodelling.

https://arctichealth.org/en/permalink/ahliterature15632
Source
Eur Respir J. 1999 Sep;14(3):633-41
Publication Type
Article
Date
Sep-1999
Author
M. Salmon
D A Walsh
H. Koto
P J Barnes
K F Chung
Author Affiliation
Thoracic Medicine, Imperial College School of Medicine at the National Heart and Lung Institute, London, UK.
Source
Eur Respir J. 1999 Sep;14(3):633-41
Date
Sep-1999
Language
English
Publication Type
Article
Keywords
Actins - metabolism
Allergens - pharmacology
Animals
Antigens, CD2 - metabolism
Asthma - chemically induced - metabolism - pathology
Blood Proteins - metabolism
Bromodeoxyuridine - metabolism
Bronchial Hyperreactivity - chemically induced - metabolism - pathology
Comparative Study
DNA - biosynthesis
Disease Models, Animal
Eosinophil Granule Proteins
Eosinophils - metabolism - pathology
Epithelial Cells - metabolism - pathology
Lung - drug effects - metabolism - pathology
Male
Muscle, Smooth - metabolism - pathology
Ovalbumin - pharmacology
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Ribonucleases
T-Lymphocytes - metabolism - pathology
Abstract
Chronic inflammation in asthmatic airways can lead to characteristic airway smooth muscle (ASM) thickening and pathological changes within the airway wall. This study assessed the effect of repeated allergen exposure on ASM and epithelial cell deoxyribonucleic acid (DNA) synthesis, cell recruitment and airway wall pathology. Brown-Norway rats were sensitized and then exposed to ovalbumin or saline aerosol every 3 days on six occasions. After the final exposure, rats were administered twice daily for 7 days with the DNA S-phase marker bromodeoxyuridine (BrdU). Using a triple immunohistochemical staining technique, BrdU incorporation into ASM and epithelium was quantified employing computer-assisted image analysis. There were >3-fold mean increases in BrdU incorporation into ASM from 1.3% of cells (95% confidence interval (CI) 1.0-1.6) in saline controls to 4.7% (95% CI 2.6-6.7) after allergen exposure (p
PubMed ID
10543287 View in PubMed
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