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Characterization of the bacterial communities on recent Icelandic volcanic deposits of different ages.

https://arctichealth.org/en/permalink/ahliterature299615
Source
BMC Microbiol. 2018 09 24; 18(1):122
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Date
09-24-2018
Author
Bo Byloos
Pieter Monsieurs
Mohamed Mysara
Natalie Leys
Nico Boon
Rob Van Houdt
Author Affiliation
Microbiology Unit, Interdisciplinary Biosciences, Belgian Nuclear Research Centre, SCKā€¢CEN, Boeretang 200, B-2400, Mol, Belgium.
Source
BMC Microbiol. 2018 09 24; 18(1):122
Date
09-24-2018
Language
English
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Keywords
Bacteria - classification - genetics - growth & development - isolation & purification
Biodiversity
Carbon - analysis - metabolism
DNA, Bacterial - genetics
Iceland
Nitrogen - analysis - metabolism
Phylogeny
RNA, Ribosomal, 16S - genetics
Soil Microbiology
Volcanic Eruptions - analysis
Abstract
Basalt is the most common igneous rock on the Earth's surface covering. Basalt-associated microorganisms drive the cycling and sequestration of different elements such as nitrogen, carbon and other nutrients, which facilitate subsequent pioneer and plant development, impacting long-term regulation of the Earth's temperature and biosphere. The initial processes of colonization and subsequent rock weathering by microbial communities are still poorly understood and relatively few data are available on the diversity and richness of the communities inhabiting successive and chronological lava flows. In this study, the bacterial communities present on lava deposits from different eruptions of the 1975-84 Krafla Fires (32-, 35- and 39-year old, respectively) at the Krafla, Iceland, were determined.
Three sites were sampled for each deposit (32-, 35- and 39-year old), two proximal sites (at 10 m distance) and one more distant site (at 100 m from the two other sites). The determined chemical composition and metal concentrations were similar for the three basalt deposits. No significant differences were observed in the total number of cells in each flow. 16S rRNA gene amplicon sequencing showed that the most abundant classified phylum across the 3 flows was Proteobacteria, although predominance of Acidobacteria, Actinobacteria and Firmicutes was observed for some sampling sites. In addition, a considerable fraction of the operational taxonomic units remained unclassified. Alpha diversity (Shannon, inverse Simpson and Chao), HOMOVA and AMOVA only showed a significant difference for Shannon between the 32- and 39-year old flow (p 
PubMed ID
30249184 View in PubMed
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PHB-degrading bacteria isolated from the gastrointestinal tract of aquatic animals as protective actors against luminescent vibriosis.

https://arctichealth.org/en/permalink/ahliterature96482
Source
FEMS Microbiol Ecol. 2010 Jun 7;
Publication Type
Article
Date
Jun-7-2010
Author
Yiying Liu
Peter De Schryver
Bart Van Delsen
Loïs Maignien
Nico Boon
Patrick Sorgeloos
Willy Verstraete
Peter Bossier
Tom Defoirdt
Author Affiliation
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Rozier, Ghent, Belgium.
Source
FEMS Microbiol Ecol. 2010 Jun 7;
Date
Jun-7-2010
Language
English
Publication Type
Article
Abstract
Abstract The use of poly-beta-hydroxybutyrate (PHB) was shown to be successful in increasing the resistance of brine shrimp against pathogenic infections. In this study, we isolated for the first time PHB-degrading bacteria from a gastrointestinal environment. Pure strains of PHB-degrading bacteria were isolated from Siberian sturgeon, European sea bass and giant river prawn. The capability of selected isolates to degrade PHB was confirmed in at least two of three setups: (1) growth in minimal medium containing PHB as the sole carbon (C) source, (2) production of clearing zones on minimal agar containing PHB as the sole C source and (3) degradation of PHB (as determined by HPLC analysis) in 10% Luria-Bertani medium containing PHB. Challenge tests showed that the PHB-degrading activity of the selected isolates increased the survival of brine shrimp larvae challenged to a pathogenic Vibrio campbellii strain by a factor 2-3. Finally, one of the PHB-degrading isolates from sturgeon showed a double biocontrol effect because it was also able to inactivate acylhomoserine lactones, a type of quorum-sensing molecule that regulates the virulence of different pathogenic bacteria. Thus, the combined supplementation of a PHB-degrading bacterium and PHB as a synbioticum provides perspectives for improving the gastrointestinal health of aquatic animals.
PubMed ID
20597982 View in PubMed
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