The dynamics of the acid and neutral proteinases general enzymes activity change in the hepatocytes postnuclear fraction in the rats suffering from the Heren's carcinoma was investigated. It was determined that in the tumor development of the enzyme activity level of both the acid and neutral proteinases increased 2,6-fold. The natural preparation of the herbs (Calendula officinalis L., Echinacea purpurea L., Scorzonera humilis L., Aconitum moldavicum Hacq.) normalizes both the activity of the investigated enzymes and coefficients of the liver weights of the sick animals. The chemical medicinal preparation 5,6-benzcumarine-5-uracil normalizes the activity of the neutral cytoplasmatic proteinases and reduces the level of the proteolytic activity of the acid enzymes in comparison with the control group of the animals as well as increases of the liver weight coefficients.
The contents of primary and secondary (TBA-active) products of lipid peroxidation were investigated in microsomal fraction of the liver and tumor cells of rats with transplanted Guerin's carcinoma and under the condition of antitumor liposomal preparation 5,6-benzcumarine-5-uracil (BCU) action. High level of lipid peroxidation process in the microsomal fraction is shown in the rat liver and tumor cells under the condition of BCU action in the period of intensive carcinoma growth. It remains till the period of tumor growth braking. This fact testifies to the prooxidation action of the preparation. Liposomal antitumor preparation BCU raises the process of lipid peroxidation in microsomal fraction of tumor cells and its action increases according to the malignant growth. The processes of lipid peroxidation in microsomal rat liver fraction approach the control data under the condition of the mentioned preparation. The investigated liposomal form of BCU possesses directed prooxidation action on the malignant tissue.
The fractionated X-ray irradiation resulted in accumulation of malonic dialdehyde (MDA) in chromatin of the cells of rat liver and activation of nuclear endogenous histon-specific proteases. Seven days after the termination of irradiation, the activity of the nuclear proteases returned to the control level and the MDA level remained 10% above the control parameter. The correlation (r = 0.71) of the processes of MDA accumulation in chromatin and activation of histon-specific proteases in the cells of rat liver after the termination of irradiation showed that these processes were coupled. Transplantation of Guerin's carcinoma to the irradiated animals caused the changes in nuclear proteolysis of the histones and MDA level in chromatin of the tumor-carrier liver cells, which lessened the influence of preliminary X-ray irradiation on rats.
The rate of proteins oxidative modification of chromatin in the liver and Heren's carcinoma of rats was studied. It was shown that the tumour development was accompanied by the changes of histones and nonhistones carbonyl derivatives levels both in the tumour, and in the liver. The processes of free-radicals oxidation of researched proteins are sensitive to the fortnight introduction of 5,6-benzcumarine-5-uracil and natural herbs preparation, resulting in the inhibition of tumour development.
The most substantial change in the ratio between glycoprotein fractions distributing in the blood serum of rat under conditions of different vitamin A provision (0.50-0.70 micromol/L) are observed; they are characteristic of gamma-globulin, haptoglobin and alpha 1-acid glycoprotein; the intensity of colouring of their bars decreases on serum electrophoregrammes. At the same time, the decline of intensity of bar coloration of ceruloplasmin fraction takes place under the conditions of middle vitamin A deficiency (0.35-0.50 micromol/L) while that of transferrin takes place at marginal A-deficient state (
The work deals with the experimental researches of lipids peroxidation in the rats liver and reduced glutatione and some antioxidant enzymes activity in the same animals blood and liver in dependence on the Heren's carcinoma dynamics as well as in the tumor-carriers ones injected subcutaneously by "NIKA" herbal preparation. As the result of the experiments it was identified, that in the course of the tumor growing there was an increase of the rats liver level of malonic dialdehyde--the final product of the lipids peroxidation, as well as of some antioxidant enzymes activity growth in the blood and liver. In the 21-th day following the tumor implantation the animals-tumor-carriers antioxidant system is exhausted. The tested herbal medicine displays the normalizing and correcting action on the rats organism protective system and provides for the tumor growth slowering.