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[Activity of cytoplasmic proteinases from rat liver in Heren's carcinoma during tumor growth and treatment with medicinal herbs]

https://arctichealth.org/en/permalink/ahliterature20009
Source
Ukr Biokhim Zh. 2000 May-Jun;72(3):91-4
Publication Type
Article
Author
M M Marchenko
H P Kopyl'chuk
O V Hrygor'ieva
Author Affiliation
Yu. Fedkovich Chernivtsi State University, Ukraine.
Source
Ukr Biokhim Zh. 2000 May-Jun;72(3):91-4
Language
Ukrainian
Publication Type
Article
Keywords
Animals
Cytoplasm - enzymology
English Abstract
Hydrolysis
Liver - enzymology - pathology
Liver Neoplasms - enzymology
Organ Size - drug effects
Plant Extracts - pharmacology
Plants, Medicinal - chemistry
Rats
Abstract
The dynamics of the acid and neutral proteinases general enzymes activity change in the hepatocytes postnuclear fraction in the rats suffering from the Heren's carcinoma was investigated. It was determined that in the tumor development of the enzyme activity level of both the acid and neutral proteinases increased 2,6-fold. The natural preparation of the herbs (Calendula officinalis L., Echinacea purpurea L., Scorzonera humilis L., Aconitum moldavicum Hacq.) normalizes both the activity of the investigated enzymes and coefficients of the liver weights of the sick animals. The chemical medicinal preparation 5,6-benzcumarine-5-uracil normalizes the activity of the neutral cytoplasmatic proteinases and reduces the level of the proteolytic activity of the acid enzymes in comparison with the control group of the animals as well as increases of the liver weight coefficients.
PubMed ID
11200483 View in PubMed
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[Effect of liposomal antitumor preparation 5,6-benzocoumarin-5-uracil on intensity of free-radical processes in the liver microsomes and tumor cells of rats with transplanted Guerin's carcinoma]

https://arctichealth.org/en/permalink/ahliterature16594
Source
Ukr Biokhim Zh. 2005 Mar-Apr;77(2):141-6
Publication Type
Article
Author
M M Marchenko
O V Ketsa
Source
Ukr Biokhim Zh. 2005 Mar-Apr;77(2):141-6
Language
Ukrainian
Publication Type
Article
Keywords
Animals
Antineoplastic Agents - administration & dosage - pharmacology - therapeutic use
Cell Line, Tumor
Coumarins - administration & dosage - pharmacology - therapeutic use
English Abstract
Free Radicals - metabolism
Lipid Peroxidation - drug effects
Lipid Peroxides - chemistry - metabolism
Liposomes
Male
Microsomes, Liver - drug effects - metabolism
Neoplasm Transplantation
Neoplasms, Experimental - drug therapy - metabolism
Rats
Thiobarbituric Acid Reactive Substances - chemistry - metabolism
Uracil - administration & dosage - analogs & derivatives - pharmacology - therapeutic use
Abstract
The contents of primary and secondary (TBA-active) products of lipid peroxidation were investigated in microsomal fraction of the liver and tumor cells of rats with transplanted Guerin's carcinoma and under the condition of antitumor liposomal preparation 5,6-benzcumarine-5-uracil (BCU) action. High level of lipid peroxidation process in the microsomal fraction is shown in the rat liver and tumor cells under the condition of BCU action in the period of intensive carcinoma growth. It remains till the period of tumor growth braking. This fact testifies to the prooxidation action of the preparation. Liposomal antitumor preparation BCU raises the process of lipid peroxidation in microsomal fraction of tumor cells and its action increases according to the malignant growth. The processes of lipid peroxidation in microsomal rat liver fraction approach the control data under the condition of the mentioned preparation. The investigated liposomal form of BCU possesses directed prooxidation action on the malignant tissue.
PubMed ID
16335247 View in PubMed
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[Influence of small doses of fractionated X-ray radiation on the activity of histone-specific proteases and the content of malonic dialdehyde in chromatin of the cells of rat liver]

https://arctichealth.org/en/permalink/ahliterature18771
Source
Radiats Biol Radioecol. 2002 Sep-Oct;42(5):488-91
Publication Type
Article
Author
M M Marchenko
G P Kopyl'chuk
E V Grigor'eva
Author Affiliation
Chernovtsy National University, Chernovtsy, 58002 Ukraine. mmarch@chnu.cv.ua
Source
Radiats Biol Radioecol. 2002 Sep-Oct;42(5):488-91
Language
Russian
Publication Type
Article
Keywords
Animals
Chromatin - metabolism - radiation effects
Comparative Study
Dose Fractionation
Electrophoresis
Endopeptidases - metabolism - radiation effects
English Abstract
Hepatocytes - radiation effects
Histones - analysis - radiation effects
Liver - cytology - metabolism - radiation effects
Male
Malondialdehyde - analysis - radiation effects
Neoplasm Transplantation
Neoplasms, Experimental - metabolism - pathology
Rats
Time Factors
Abstract
The fractionated X-ray irradiation resulted in accumulation of malonic dialdehyde (MDA) in chromatin of the cells of rat liver and activation of nuclear endogenous histon-specific proteases. Seven days after the termination of irradiation, the activity of the nuclear proteases returned to the control level and the MDA level remained 10% above the control parameter. The correlation (r = 0.71) of the processes of MDA accumulation in chromatin and activation of histon-specific proteases in the cells of rat liver after the termination of irradiation showed that these processes were coupled. Transplantation of Guerin's carcinoma to the irradiated animals caused the changes in nuclear proteolysis of the histones and MDA level in chromatin of the tumor-carrier liver cells, which lessened the influence of preliminary X-ray irradiation on rats.
PubMed ID
12449814 View in PubMed
Less detail

[Oxidative modification of proteins in rat liver and Heren's carcinoma chromatin]

https://arctichealth.org/en/permalink/ahliterature18963
Source
Ukr Biokhim Zh. 2002 Mar-Apr;74(2):111-4
Publication Type
Article
Author
H P Kopyl'chuk
L V Khuda
M M Marchenko
Author Affiliation
Fedkovich Chernivtsy National University, Ukraine.
Source
Ukr Biokhim Zh. 2002 Mar-Apr;74(2):111-4
Language
Ukrainian
Publication Type
Article
Keywords
Animals
Chromatin - metabolism
English Abstract
Liver - metabolism
Liver Neoplasms, Experimental - metabolism
Oxidation-Reduction
Rats
Abstract
The rate of proteins oxidative modification of chromatin in the liver and Heren's carcinoma of rats was studied. It was shown that the tumour development was accompanied by the changes of histones and nonhistones carbonyl derivatives levels both in the tumour, and in the liver. The processes of free-radicals oxidation of researched proteins are sensitive to the fortnight introduction of 5,6-benzcumarine-5-uracil and natural herbs preparation, resulting in the inhibition of tumour development.
PubMed ID
12152301 View in PubMed
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[Peculiarities of fractional composition of serum glycoproteins in Guerin's carcinoma development under condition of different vitamin A provision]

https://arctichealth.org/en/permalink/ahliterature98798
Source
Ukr Biokhim Zh. 2009 Jan-Feb;81(1):89-98
Publication Type
Article
Author
M M Marchenko
I O Shmarakov
M V Pasailiuk
Source
Ukr Biokhim Zh. 2009 Jan-Feb;81(1):89-98
Language
Ukrainian
Publication Type
Article
Keywords
Animals
Electrophoresis, Polyacrylamide Gel
Female
Glycoproteins - blood - chemistry
Neoplasm Transplantation
Neoplasms, Experimental - blood - complications
Rats
Vitamin A - administration & dosage
Vitamin A Deficiency - blood - complications
Abstract
The most substantial change in the ratio between glycoprotein fractions distributing in the blood serum of rat under conditions of different vitamin A provision (0.50-0.70 micromol/L) are observed; they are characteristic of gamma-globulin, haptoglobin and alpha 1-acid glycoprotein; the intensity of colouring of their bars decreases on serum electrophoregrammes. At the same time, the decline of intensity of bar coloration of ceruloplasmin fraction takes place under the conditions of middle vitamin A deficiency (0.35-0.50 micromol/L) while that of transferrin takes place at marginal A-deficient state (
PubMed ID
19877421 View in PubMed
Less detail

[Status of the rat antioxidant system and correction by medicinal plant preparations during the dynamics of growth of Heren's carcinoma]

https://arctichealth.org/en/permalink/ahliterature20440
Source
Ukr Biokhim Zh. 1999 Nov-Dec;71(6):76-80
Publication Type
Article
Author
M M Marchenko
I D Nykoliuk
V V Bevzo
Author Affiliation
Yu. Fedkovich State University, Chernivtsy, Ukraine.
Source
Ukr Biokhim Zh. 1999 Nov-Dec;71(6):76-80
Language
Ukrainian
Publication Type
Article
Keywords
Animals
Antioxidants - metabolism
Cell Division - drug effects
English Abstract
Female
Lipid Peroxidation - drug effects
Liver - drug effects - metabolism
Malondialdehyde - metabolism
Neoplasm Transplantation
Neoplasms, Experimental - metabolism - pathology
Plant Extracts - pharmacology
Plants, Medicinal - chemistry
Rats
Abstract
The work deals with the experimental researches of lipids peroxidation in the rats liver and reduced glutatione and some antioxidant enzymes activity in the same animals blood and liver in dependence on the Heren's carcinoma dynamics as well as in the tumor-carriers ones injected subcutaneously by "NIKA" herbal preparation. As the result of the experiments it was identified, that in the course of the tumor growing there was an increase of the rats liver level of malonic dialdehyde--the final product of the lipids peroxidation, as well as of some antioxidant enzymes activity growth in the blood and liver. In the 21-th day following the tumor implantation the animals-tumor-carriers antioxidant system is exhausted. The tested herbal medicine displays the normalizing and correcting action on the rats organism protective system and provides for the tumor growth slowering.
PubMed ID
10820852 View in PubMed
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6 records – page 1 of 1.