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Toward a rapid method for the study of biodiversity in cold environments: the characterization of psychrophilic yeasts by MALDI-TOF mass spectrometry.

https://arctichealth.org/en/permalink/ahliterature299993
Source
Extremophiles. 2019 May 14; :
Publication Type
Journal Article
Date
May-14-2019
Author
Joseph J Dalluge
Elizabeth C Brown
Laurie B Connell
Author Affiliation
Department of Chemistry, University of Minnesota, Minneapolis, MN, 55455, USA. jdalluge@umn.edu.
Source
Extremophiles. 2019 May 14; :
Date
May-14-2019
Language
English
Publication Type
Journal Article
Abstract
To investigate the potential of matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF/MS) as a platform to support biodiversity and phylogenetic studies of psychrophilic yeasts in cold environments, the technique was employed to rapidly characterize and distinguish three psychrophilic yeasts (Rhodotorula mucilaginosa, Naganishia vishniacii, and Dioszegia cryoxerica) from three mesophilic counterparts (Saccharomyces cerevisiae Cry Havoc, S. cerevisiae California V Ale, and S. pastorianus). A detailed workflow for providing reproducible mass spectral fingerprints of low molecular weight protein/peptide features specific to the organisms studied is presented. The potential of this approach as a tool in the study of biodiversity, systematics, and phylogeny of psychrophilic microorganisms is highlighted.
PubMed ID
31089891 View in PubMed
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