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Tracing Mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting: a retrospective population-based study in East Greenland.

https://arctichealth.org/en/permalink/ahliterature292531
Source
Sci Rep. 2016 09 12; 6:33180
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Date
09-12-2016
Author
K Bjorn-Mortensen
B Soborg
A Koch
K Ladefoged
M Merker
T Lillebaek
A B Andersen
S Niemann
T A Kohl
Author Affiliation
Department of Epidemiology Research, Statens Serum Institut, Copenhagen, Denmark.
Source
Sci Rep. 2016 09 12; 6:33180
Date
09-12-2016
Language
English
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Keywords
Adolescent
Adult
Child
Female
Genotype
Greenland - epidemiology
Humans
Incidence
Male
Molecular Typing
Mycobacterium tuberculosis - genetics
Retrospective Studies
Tuberculosis, Pulmonary - epidemiology - microbiology - transmission
Whole Genome Sequencing
Young Adult
Abstract
In East Greenland, a dramatic increase of tuberculosis (TB) incidence has been observed in recent years. Classical genotyping suggests a genetically similar Mycobacterium tuberculosis (Mtb) strain population as cause, however, precise transmission patterns are unclear. We performed whole genome sequencing (WGS) of Mtb isolates from 98% of culture-positive TB cases through 21?years (n?=?182) which revealed four genomic clusters of the Euro-American lineage (mainly sub-lineage 4.8 (n?=?134)). The time to the most recent common ancestor of lineage 4.8 strains was found to be 100?years. This sub-lineage further diversified in the 1970s, and massively expanded in the 1990s, a period of lowered TB awareness in Greenland. Despite the low genetic strain diversity, WGS data revealed several recent short-term transmission events in line with the increasing incidence in the region. Thus, the isolated setting and the uniformity of circulating Mtb strains indicated that the majority of East Greenlandic TB cases originated from one or few strains introduced within the last century. Thereby, the study shows the consequences of even short interruptions in TB control efforts in previously TB high incidence areas and demonstrates the potential role of WGS in detecting ongoing micro epidemics, thus guiding public health efforts in the future.
Notes
Cites: Int J Circumpolar Health. 2004;63 Suppl 2:221-4 PMID 15736656
Cites: Nat Genet. 2013 Oct;45(10):1176-82 PMID 23995134
Cites: Lancet Infect Dis. 2013 Feb;13(2):137-46 PMID 23158499
Cites: Eur Respir J. 2015 Sep;46(3):865-9 PMID 25882797
Cites: J Clin Microbiol. 1994 Dec;32(12):3018-25 PMID 7883893
Cites: Proc Natl Acad Sci U S A. 2015 Nov 3;112(44):13609-14 PMID 26483462
Cites: J Clin Microbiol. 2006 Dec;44(12):4498-510 PMID 17005759
Cites: J Clin Microbiol. 2015 Aug;53(8):2716-9 PMID 26019203
Cites: PLoS Med. 2013;10(2):e1001387 PMID 23424287
Cites: Mol Biol Evol. 2012 Aug;29(8):1969-73 PMID 22367748
Cites: Elife. 2015 Mar 03;4:null PMID 25732036
Cites: Arctic Med Res. 1988 Jan;47(1):22-6 PMID 3285852
Cites: Genome Biol. 2014 Nov 07;15(11):490 PMID 25418686
Cites: PLoS One. 2014 Nov 18;9(11):e112928 PMID 25405861
Cites: J Clin Microbiol. 2013 Dec;51(12):4040-4 PMID 24068008
Cites: Nat Genet. 2011 May;43(5):482-6 PMID 21516081
Cites: Bioinformatics. 2011 May 15;27(10):1351-8 PMID 21450712
Cites: J Infect Dis. 2015 Jun 15;211(12):1905-14 PMID 25576599
Cites: Lancet Respir Med. 2014 Apr;2(4):285-92 PMID 24717625
Cites: PLoS One. 2009 Nov 12;4(11):e7815 PMID 19915672
Cites: Thorax. 2014 Sep;69(9):851-6 PMID 24969643
Cites: PLoS One. 2013 Dec 06;8(12):e82551 PMID 24324807
Cites: Nat Commun. 2014 Sep 01;5:4812 PMID 25176035
Cites: Ugeskr Laeger. 1996 Jul 29;158(31):4399-402 PMID 8759997
Cites: J Clin Microbiol. 2008 Aug;46(8):2692-9 PMID 18550737
Cites: Semin Immunol. 2014 Dec;26(6):431-44 PMID 25453224
Cites: BMC Infect Dis. 2013 Feb 27;13:110 PMID 23446317
Cites: J Infect Dis. 2014 Jan 1;209(1):98-108 PMID 23945373
PubMed ID
27615360 View in PubMed
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