1] Institute for Cancer Genetics and Informatics, Oslo University Hospital, PO Box 4950 Nydalen, 0424 Oslo, Norway  Centre for Cancer Biomedicine, University of Oslo, PO Box 4950 Nydalen, 0424 Oslo, Norway  Department of Informatics, University of Oslo, PO Box 1080 Blindern, 0316 Oslo, Norway.
The prognostic impact of an indication of chromosomal instability (CIN) is evaluated in a consecutive series of 952 colorectal cancer patients treated at Aker University Hospital, Norway, during 1993-2003. Microsatellite instability (MSI) in this case series has recently been reported and made it possible to find the co-occurrence and compare the prognostic significance of CIN and MSI.
Data sets for overall survival (OS; n=855) and time to recurrence (TTR; n=579) were studied. To reveal CIN we used automated image cytometry (ICM). Non-diploid histograms were taken as indicative of the presence of CIN. PCR-based measures of MSI in this material have already been described.
As with MSI, CIN was found to be an independent predictor of early relapse and death among stage II patients (TTR: n=278: HR 2.19 (95% CI: 1.35-3.55), P=0.002). Of the MSI tumours (16%), 71% were found to be DNA diploid, 21% were DNA tetraploid and 8% were DNA aneuploid. Among microsatellite stable tumours, 24% were DNA diploid, 15% were DNA tetraploid and 61% were DNA aneuploid.
For patients presenting with stage II disease, genomic instability as detected by DNA image cytometry has the potential to provide a useful biomarker for relapse and cancer-related death following surgery with curative intent.
BACKGROUND: The article gives an overview of the telepathology activity at the Norwegian Radium Hospital from the service was launched in 1994 and up until today. We show the development during these years and discuss telepathology in general terms. We also discuss those aspects that determine how well a telepathology service functions. MATERIAL AND METHODS: 74 frozen section slides were diagnosed by two different telepathology systems. One of these systems was used for examining its appropriateness as a tool for second opinion in pathology. A new Internet-based system was developed that provided additional functionality. RESULTS: A telepathology system with a digital camera outperforms one with an analog camera with respect to diagnostic accuracy. INTERPRETATION: Image quality determines the precision of a telepathology service. Telepathology is a feasible tool for second opinion in pathology.
Comment In: Tidsskr Nor Laegeforen. 2000 Sep 10;120(21):257911071002