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[Analysis of complete sequence of cryptic plasmid pTP33 from Yersinia pestis isolated in Tuva natural focus of plague].

https://arctichealth.org/en/permalink/ahliterature289526
Source
Genetika. 2016 Sep; 52(9):1012-20
Publication Type
Journal Article
Date
Sep-2016
Author
M V Afanas’ev
S V Balakhonov
E G Tokmakova
V S Polovinkina
E A Sidorova
V V Sinkov
Source
Genetika. 2016 Sep; 52(9):1012-20
Date
Sep-2016
Language
Russian
Publication Type
Journal Article
Keywords
Bacterial Proteins - genetics
Plague - genetics
Plasmids - genetics
Siberia
Yersinia pestis - genetics - isolation & purification
Abstract
This paper studies a full nucleotide sequence of cryptic plasmid pTP33, which was isolated from the typical plague strain of the Tuvinian natural focus, Yersinia pestis I-2638. Sequencing was carried out using the 454 GS Junior platform (Roche). In analysis using the software package GS De Novo Assembler v. 2.7 (Roche) and the algorithm Newbler v. 2.7, 1855 nucleotide reads, which contained 1101246 nucleotides, were assembled to a contig of 33 978 bp. The GC content of the obtained nucleotide sequence was 50.25%. During annotation, we found 56 open reading frames. Homologs of the predicted reading frames were sought in the BLAST databases. We detected 22 reading frames coding hypothetical proteins, 23 frames coding phagerelated proteins, and 11 frames coding proteins with known functions, including toxin–antitoxin system YefM-YoeB, nucleic acids and polysaccharides metabolism proteins (exopolysaccharide production protein ExoZ, exodeoxyribonuclease VIII), and replication proteins (ParA). Some predicted pTP33 proteins were found to be homologs (from 45 to 75%) with sequences of phage-related proteins of certain microorganisms—endosymbionts of insects (Sodalis glossinidius) and endosymbionts of entomopathogenic nematodes (Photorhabdus luminescens, P. asymbiotica, Xenorhabdus bovienii).
PubMed ID
29369556 View in PubMed
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[Analysis of complete sequence of cryptic plasmid pTP33 from Yersinia pestis isolated in Tuva natural focus of plague].

https://arctichealth.org/en/permalink/ahliterature289684
Source
Genetika. 2016 Sep; 52(9):1012-20
Publication Type
Journal Article
Date
Sep-2016
Author
M V Afanas’ev
S V Balakhonov
E G Tokmakova
V S Polovinkina
E A Sidorova
V V Sinkov
Source
Genetika. 2016 Sep; 52(9):1012-20
Date
Sep-2016
Language
Russian
Publication Type
Journal Article
Keywords
Bacterial Proteins - genetics
Plague - genetics
Plasmids - genetics
Siberia
Yersinia pestis - genetics - isolation & purification
Abstract
This paper studies a full nucleotide sequence of cryptic plasmid pTP33, which was isolated from the typical plague strain of the Tuvinian natural focus, Yersinia pestis I-2638. Sequencing was carried out using the 454 GS Junior platform (Roche). In analysis using the software package GS De Novo Assembler v. 2.7 (Roche) and the algorithm Newbler v. 2.7, 1855 nucleotide reads, which contained 1101246 nucleotides, were assembled to a contig of 33 978 bp. The GC content of the obtained nucleotide sequence was 50.25%. During annotation, we found 56 open reading frames. Homologs of the predicted reading frames were sought in the BLAST databases. We detected 22 reading frames coding hypothetical proteins, 23 frames coding phagerelated proteins, and 11 frames coding proteins with known functions, including toxin–antitoxin system YefM-YoeB, nucleic acids and polysaccharides metabolism proteins (exopolysaccharide production protein ExoZ, exodeoxyribonuclease VIII), and replication proteins (ParA). Some predicted pTP33 proteins were found to be homologs (from 45 to 75%) with sequences of phage-related proteins of certain microorganisms—endosymbionts of insects (Sodalis glossinidius) and endosymbionts of entomopathogenic nematodes (Photorhabdus luminescens, P. asymbiotica, Xenorhabdus bovienii).
PubMed ID
29369556 View in PubMed
Less detail

[The sensitivity of plague agent from Siberian natural focuses of disease to antibacterial preparations in vitro].

https://arctichealth.org/en/permalink/ahliterature107691
Source
Klin Lab Diagn. 2013 Apr;(4):36-40
Publication Type
Article
Date
Apr-2013
Author
S V Balakhonov
S A Bel'kova
E G Tokmakova
I G Khvoinova
O D Zakhlebnaia
Source
Klin Lab Diagn. 2013 Apr;(4):36-40
Date
Apr-2013
Language
Russian
Publication Type
Article
Keywords
Anti-Bacterial Agents - pharmacology
Culture Media
Disease Reservoirs
Humans
Microbial Sensitivity Tests
Plague - drug therapy - genetics - microbiology
Siberia
Yersinia pestis - drug effects - growth & development - pathogenicity
Abstract
The comparative analysis was applied concerning antimicrobic action of different groups of antibacterial preparations on the plague agent strains isolated from Siberian natural focuses of disease. The analysis was applied to results obtained using such different methods as disco-diffusive technique, serial dilution and HiCOMB MIC test. It is established that freshly isolated cultures of Yersinia pestis have high sensitivity to antibacterial preparations of different groups. The results obtained using three technical approaches intercorrelate in significant degree. The HiCOMB MIC test was applied for the first time for analysis Y. pestis strains from natural focuses of plague of Siberia.
PubMed ID
23984554 View in PubMed
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