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Assessing the diversity of the g23 gene of T4-like bacteriophages from Lake Baikal with high-throughput sequencing.

https://arctichealth.org/en/permalink/ahliterature287595
Source
FEMS Microbiol Lett. 2017 Dec 05;
Publication Type
Article
Date
Dec-05-2017
Author
Sergey Potapov
Olga Olga
Andrey Krasnopeev
Anna Gladkikh
Marsel Kabilov
Aleksey Tupikin
Tatyana V Butina
Source
FEMS Microbiol Lett. 2017 Dec 05;
Date
Dec-05-2017
Language
English
Publication Type
Article
Abstract
Based on second generation sequencing (MiSeq platform), we determined the genetic diversity of T4-like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obtained by the Sanger method before. We obtained 33701 sequences of the g23 gene fragments, 141 OTUs of which were identified. 86 OTUs (60.9%) had the closest relatives from lakes Bourget and Annecy, and 28 OTUs (19.8%) had the highest identity with the Baikal g23 clones, which had been previously identified in the northern and southern basins of the lake by the Sanger method. The remaining OTUs were similar to the clones from other ecosystems. We showed a high genetic diversity of T4-type bacteriophages and a genetic difference with the phage communities from other ecosystems.
PubMed ID
29228190 View in PubMed
Less detail

Assessing the diversity of the g23 gene of T4-like bacteriophages from Lake Baikal with high-throughput sequencing.

https://arctichealth.org/en/permalink/ahliterature303053
Source
FEMS Microbiol Lett. 2018 02 01; 365(3):
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Date
02-01-2018
Author
Sergey Potapov
Olga Belykh
Andrey Krasnopeev
Anna Gladkikh
Marsel Kabilov
Aleksey Tupikin
Tatyana Butina
Author Affiliation
Limnological Institute Siberian Branch of the Russian Academy of Sciences, 3 Ulan-Batorskaya, Irkutsk 664033, Russia.
Source
FEMS Microbiol Lett. 2018 02 01; 365(3):
Date
02-01-2018
Language
English
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Keywords
Bacteriophage T4 - classification - genetics
Capsid Proteins - genetics
Genes, Viral - genetics
Genetic Variation
High-Throughput Nucleotide Sequencing
Lakes - virology
Phylogeny
Plankton - classification - genetics
Sequence Analysis, DNA
Water Microbiology
Abstract
Based on second generation sequencing (MiSeq platform, Illumina), we determined the genetic diversity of T4-like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obtained by the Sanger method before. We obtained 33 701 sequences of the g23 gene fragments, 141 operational taxonomic units (OTUs) of which were identified. 86 OTUs (60.9%) had the closest relatives from lakes Bourget and Annecy, and 28 OTUs (19.8%) had the highest identity with the Baikal g23 clones, which had been previously identified in the northern and southern basins of the lake by the Sanger method. The remaining OTUs were similar to the clones from other ecosystems. We showed a high genetic diversity of T4-type bacteriophages and a genetic difference with the phage communities from other ecosystems.
PubMed ID
29228190 View in PubMed
Less detail