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Associations of Neospora caninum seropositivity with gestation number and pregnancy outcome in Danish dairy herds.

https://arctichealth.org/en/permalink/ahliterature64063
Source
Prev Vet Med. 1999 Jun 11;40(3-4):151-63
Publication Type
Article
Date
Jun-11-1999
Author
A M Jensen
C. Björkman
A M Kjeldsen
A. Wedderkopp
C. Willadsen
A. Uggla
P. Lind
Author Affiliation
Danish Agricultural Advisory Centre, Aarhus, Denmark. amj@lr.dk
Source
Prev Vet Med. 1999 Jun 11;40(3-4):151-63
Date
Jun-11-1999
Language
English
Publication Type
Article
Keywords
Abortion, Veterinary - parasitology
Animals
Antibodies, Protozoan - analysis
Antigens, Protozoan - immunology
Cattle
Cattle Diseases - epidemiology - immunology - parasitology
Coccidiosis - epidemiology - immunology - parasitology - veterinary
Dairying
Denmark - epidemiology
Enzyme-Linked Immunosorbent Assay - veterinary
Female
Fluorescent Antibody Technique, Indirect - veterinary
Litter Size
Male
Neospora - immunology
Pregnancy
Pregnancy Complications, Parasitic - epidemiology - immunology - parasitology - veterinary
Pregnancy Outcome - veterinary
Research Support, Non-U.S. Gov't
Seroepidemiologic Studies
Abstract
The prevalence and distribution of seropositivity towards the protozoan parasite Neospora caninum were studied in single blood samples from 1561 cows from 31 Danish dairy herds. Blood samples were analysed by an indirect enzyme-linked immunoassay and an indirect fluorescent-antibody test. Seroprevalence in 15 herds with previous abortions assigned to neosporosis ranged from 1% to 58%, with a mean frequency of 22%. In eight out of 16 herds without a history of N.caninum related abortions, no seroreactors were found. In the remaining eight herds, the seroprevalence ranged from 6% to 59%. The prevalence and distribution of seropositivity, gestation number prior to sampling, and breed were related to abortions and perinatal deaths using a random-effects logistic-regression model. Abortion risk was significantly increased in seropositive animals (OR = 3) and in > or = 2nd-gestation cows (OR = 3). Perinatal death was significantly influenced by gestation number and breed, but not by serostatus. Reproductive performance and culling risk of cows were not affected by serostatus. Seropositivity increased with "age" (i.e. gestation number) (P = 0.02). In open cows, seropositivity tended to decrease with distance from calving (P = 0.05). The proportion of seropositive pregnant cows increased with trimester (P = 0.02).
Notes
Erratum In: Prev Vet Med 2000 Jan 20;43(2):139-40
PubMed ID
10423771 View in PubMed
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Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark.

https://arctichealth.org/en/permalink/ahliterature75512
Source
J Appl Microbiol. 2003;94(5):929-35
Publication Type
Article
Date
2003
Author
M. Lund
A. Wedderkopp
M. Wainø
S. Nordentoft
D D Bang
K. Pedersen
M. Madsen
Author Affiliation
Danish Veterinary Institute, Arhus N, Denmark. mlu@vetinst.dk
Source
J Appl Microbiol. 2003;94(5):929-35
Date
2003
Language
English
Publication Type
Article
Keywords
Abattoirs - standards
Animals
Bacteriological Techniques - methods
Campylobacter - isolation & purification
Chickens - microbiology
DNA, Bacterial - analysis
Feces - microbiology
Food Microbiology
Polymerase Chain Reaction - methods
Reproducibility of Results
Research Support, Non-U.S. Gov't
Safety Management - methods
Sensitivity and specificity
Abstract
AIMS: To develop and evaluate a rapid and sensitive PCR method for detection of Campylobacter spp. directly from chicken faeces. METHODS AND RESULTS: DNA was isolated from faecal swabs using magnetic beads followed by PCR using a prealiquoted PCR mixture, which had been stored in the freezer. The result could be obtained in
PubMed ID
12694459 View in PubMed
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