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Analysis of Neisseria gonorrhoeae in Ontario, Canada, with decreased susceptibility to quinolones by pulsed-field gel electrophoresis, auxotyping, serotyping and plasmid content.

https://arctichealth.org/en/permalink/ahliterature208613
Source
J Med Microbiol. 1997 May;46(5):383-90
Publication Type
Article
Date
May-1997
Author
N. Harnett
S. Brown
G. Riley
R. Terro
C. Krishnan
M. Pauzé
K H Yeung
Author Affiliation
Central Public Health Laboratory, Ontario Ministry of Health, Toronto, Canada.
Source
J Med Microbiol. 1997 May;46(5):383-90
Date
May-1997
Language
English
Publication Type
Article
Keywords
4-Quinolones
Anti-Infective Agents - pharmacology
Bacterial Typing Techniques
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field
Gonorrhea - epidemiology - microbiology
Humans
Incidence
Microbial Sensitivity Tests
Neisseria gonorrhoeae - classification - drug effects
Ontario - epidemiology
Plasmids
Restriction Mapping
Serotyping
Abstract
The incidence of Neisseria gonorrhoeae with reduced susceptibility to quinolones increased from 0.18% (63 of 3285) in 1992 to 0.56% (15 of 2663) in 1993 and 0.62% (46 of 2846) in 1994. In all, 65 of the 67 isolates of Neisseria gonorrhoeae with decreased susceptibility to quinolones were characterised by pulsed-field gel electrophoresis (PFGE), auxotyping, serotyping and plasmid content. The strains were distributed among 14 auxotype/serovar (A/S) classes. Thirty isolates (46.2%) which were penicillin-susceptible with ciprofloxacin MIC90 of 0.12 mg/L and norfloxacin MIC90 of 1.0 mg/L belonged to a single A/S class, OUHL/IA-2. All but two of the 30 isolates had identical PFGE restriction profiles with NheI restriction endonuclease. Fifteen isolates (23.1%) with MICs in the intermediate (or resistant) categories for penicillin and with ciprofloxacin and norfloxacin MIC90 of 0.25 and 4.0 mg/L and (0.5 and 4.0 mg/L) respectively, belonged to A/S class P/IB-1. The 15 isolates showed nine different patterns with NheI and eight patterns with SpeI restriction endonucleases. Two of three beta-lactamase-producing (PPNG) isolates belonged to A/S class P/IB-5 and had a dissimilar PFGE restriction profile with NheI endonuclease; the other isolate belonged to A/S class P/IB-8. The remaining 17 isolates were distributed among 11 A/S classes. Three isolates within the common A/S class NR/IB-1 were subdivided into two types by PFGE as were three isolates belonging to A/S class NR/IB-2. Overall the 65 isolates of N. gonorrhoeae were distributed into 30 NheI and 26 SpeI macrorestriction profiles. All but one isolate harboured the 2.6-MDa cryptic plasmid and 18 isolates carried the 24.5-MDa transferable plasmid. The three PPNG isolates carried the 4.5-MDa Asian beta-lactamase-producing plasmid and a 25.2-MDa conjugative plasmid was found in the two TRNG isolates.
PubMed ID
9152033 View in PubMed
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Decreased susceptibility of Neisseria gonorrhoeae to fluoroquinolones--Ontario, 1992-1994.

https://arctichealth.org/en/permalink/ahliterature215796
Source
Can Commun Dis Rep. 1995 Feb 15;21(3):17-20; discussion 20-1
Publication Type
Article
Date
Feb-15-1995
Author
N. Harnett
S. Brown
G. Riley
R. Terro
C. Krishnan
Author Affiliation
STD Laboratory, Ontario Ministry of Health, Toronto.
Source
Can Commun Dis Rep. 1995 Feb 15;21(3):17-20; discussion 20-1
Date
Feb-15-1995
Language
English
French
Publication Type
Article
Keywords
Anti-Infective Agents - pharmacology
Drug Resistance, Microbial
Female
Fluoroquinolones
Gonorrhea - epidemiology - microbiology
Humans
Male
Neisseria gonorrhoeae - drug effects
Ontario - epidemiology
PubMed ID
7881379 View in PubMed
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Isolates of Neisseria gonorrhoeae in Ontario showing chromosomally-mediated resistance to penicillin combined with plasmid-mediated resistance to tetracycline.

https://arctichealth.org/en/permalink/ahliterature214550
Source
Can Commun Dis Rep. 1995 Aug 15;21(15):139-43
Publication Type
Article
Date
Aug-15-1995