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1H-NMR metabolomic biomarkers of poor outcome after hemorrhagic shock are absent in hibernators.

https://arctichealth.org/en/permalink/ahliterature267428
Source
PLoS One. 2014;9(9):e107493
Publication Type
Article
Date
2014
Author
Lori K Bogren
Carl J Murphy
Erin L Johnston
Neeraj Sinha
Natalie J Serkova
Kelly L Drew
Source
PLoS One. 2014;9(9):e107493
Date
2014
Language
English
Publication Type
Article
Keywords
Animals
Biological Markers - blood
Hibernation
Lipids - blood
Magnetic Resonance Spectroscopy
Male
Metabolome
Rats, Sprague-Dawley
Reperfusion Injury - blood - prevention & control
Sciuridae
Shock, Hemorrhagic - blood - therapy
Treatment Outcome
Abstract
Hemorrhagic shock (HS) following trauma is a leading cause of death among persons under the age of 40. During HS the body undergoes systemic warm ischemia followed by reperfusion during medical intervention. Ischemia/reperfusion (I/R) results in a disruption of cellular metabolic processes that ultimately lead to tissue and organ dysfunction or failure. Resistance to I/R injury is a characteristic of hibernating mammals. The present study sought to identify circulating metabolites in the rat as biomarkers for metabolic alterations associated with poor outcome after HS. Arctic ground squirrels (AGS), a hibernating species that resists I/R injury independent of decreased body temperature (warm I/R), was used as a negative control.
Male Sprague-Dawley rats and AGS were subject to HS by withdrawing blood to a mean arterial pressure (MAP) of 35 mmHg and maintaining the low MAP for 20 min before reperfusing with Ringers. The animals' temperature was maintained at 37 ? 0.5 ?C for the duration of the experiment. Plasma samples were taken immediately before hemorrhage and three hours after reperfusion. Hydrophilic and lipid metabolites from plasma were then analyzed via 1H-NMR from unprocessed plasma and lipid extracts, respectively. Rats, susceptible to I/R injury, had a qualitative shift in their hydrophilic metabolic fingerprint including differential activation of glucose and anaerobic metabolism and had alterations in several metabolites during I/R indicative of metabolic adjustments and organ damage. In contrast, I/R injury resistant AGS, regardless of season or body temperature, maintained a stable metabolic homeostasis revealed by a qualitative 1H-NMR metabolic profile with few changes in quantified metabolites during HS-induced global I/R.
An increase in circulating metabolites indicative of anaerobic metabolism and activation of glycolytic pathways is associated with poor prognosis after HS in rats. These same biomarkers are absent in AGS after HS with warm I/R.
Notes
Cites: Am J Physiol Regul Integr Comp Physiol. 2010 Feb;298(2):R329-4019923364
Cites: Gerontology. 2010;56(2):220-3019602865
Cites: Am J Physiol Regul Integr Comp Physiol. 2011 Nov;301(5):R1440-5221865542
Cites: Transplantation. 2011 Dec 15;92(11):1215-2122082817
Cites: Am J Respir Crit Care Med. 2011 Sep 15;184(6):647-5521680948
Cites: Pharmacol Ther. 2012 Feb;133(2):230-5522138603
Cites: Bioanalysis. 2012 Feb;4(3):321-4122303835
Cites: Resuscitation. 2012 Feb;83(2):253-821864484
Cites: Comp Biochem Physiol B Biochem Mol Biol. 2012 May;162(1-3):1-922326449
Cites: Nucleic Acids Res. 2012 Jul;40(Web Server issue):W127-3322553367
Cites: Physiol Genomics. 2012 Jul 15;44(14):717-2722643061
Cites: Resuscitation. 2012 Sep;83(9):1166-7222353638
Cites: Exp Biol Med (Maywood). 2013 May;238(5):539-4823856905
Cites: J Surg Res. 2014 Jan;186(1):338-4524124975
Cites: PLoS One. 2014;9(4):e9422524728042
Cites: Jpn J Pharmacol. 2001 Oct;87(2):143-5011700013
Cites: Am J Surg. 2001 Nov;182(5):481-511754855
Cites: J Comp Physiol B. 2010 Apr;180(4):599-61719967378
Cites: J Trauma. 2010 Jul;69(1):31-4020622576
Cites: J Surg Res. 2010 Nov;164(1):e131-920855081
Cites: Shock. 2010 Dec;34(6):565-7220386494
Cites: Comp Biochem Physiol Part D Genomics Proteomics. 2010 Dec;5(4):265-7320728417
Cites: Am J Physiol Lung Cell Mol Physiol. 2011 Jan;300(1):L4-L1120889676
Cites: J Exp Biol. 2011 Apr 15;214(Pt 8):1300-621430207
Cites: Physiol Genomics. 2011 Jul 14;43(13):799-80721540299
Cites: PLoS One. 2011;6(10):e2702122046435
Cites: Jpn J Pharmacol. 2002 May;89(1):36-4312083741
Cites: Horm Behav. 2003 Feb;43(2):318-2612694642
Cites: J Emerg Med. 2003 May;24(4):413-2212745044
Cites: Surgery. 2003 Aug;134(2):267-7412947328
Cites: Biol Reprod. 1988 Apr;38(3):616-223378074
Cites: J Comp Physiol B. 1988;158(1):25-373385059
Cites: Science. 1989 Jun 30;244(4912):1593-52740905
Cites: J Lipid Res. 1993 Jun;34(6):1009-198354948
Cites: J Cereb Blood Flow Metab. 1994 Mar;14(2):193-2058113316
Cites: Anal Chem. 1995 Mar 1;67(5):793-8117762816
Cites: J Cereb Blood Flow Metab. 1998 Feb;18(2):168-759469159
Cites: Kidney Int. 2005 Mar;67(3):1142-5115698456
Cites: Physiol Rev. 2003 Oct;83(4):1153-8114506303
Cites: Annu Rev Physiol. 2004;66:239-7414977403
Cites: Am J Physiol Gastrointest Liver Physiol. 2005 Mar;288(3):G473-8015701622
Cites: Chem Res Toxicol. 2005 Apr;18(4):639-5415833024
Cites: Annu Rev Nutr. 2005;25:469-9716011475
Cites: Anesth Analg. 2005 Dec;101(6):1577-8316301222
Cites: J Hepatol. 2006 May;44(5):956-6216223541
Cites: Stroke. 2006 May;37(5):1261-516574920
Cites: Surgery. 2006 Sep;140(3):404-1216934602
Cites: Am J Physiol Gastrointest Liver Physiol. 2006 Nov;291(5):G895-90116751173
Cites: J Surg Res. 2007 Jan;137(1):96-10217064732
Cites: JPEN J Parenter Enteral Nutr. 2007 Mar-Apr;31(2):94-10017308249
Cites: Bioessays. 2007 May;29(5):431-4017450592
Cites: Acta Cardiol. 2007 Aug;62(4):381-917824299
Cites: Physiol Genomics. 2007 Sep 19;31(1):15-2417536023
Cites: J Cereb Blood Flow Metab. 2008 Jul;28(7):1307-1918398417
Cites: J Neural Transm (Vienna). 2008 Jul;115(7):1011-718478178
Cites: Am J Physiol Regul Integr Comp Physiol. 2008 Jul;295(1):R316-2818434441
Cites: Allergol Int. 2008 Sep;57(3):211-718566550
Cites: Crit Care. 2008;12(4):21818638356
Cites: Shock. 2009 Jan;31(1):40-918497709
Cites: Am J Physiol Regul Integr Comp Physiol. 2009 Feb;296(2):R383-9319052316
Cites: Conf Proc IEEE Eng Med Biol Soc. 2008;2008:4891-419163813
Cites: Brain Res Rev. 2009 Mar;59(2):293-31518845187
Cites: Nucleic Acids Res. 2009 Jul;37(Web Server issue):W652-6019429898
Cites: J Neurochem. 2009 Aug;110(4):1170-919493168
Cites: Exp Biol Med (Maywood). 2009 Sep;234(9):1011-919546346
Cites: Mol Biosyst. 2010 Jan;6(1):215-2420024083
PubMed ID
25211248 View in PubMed
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