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Ellagitannins of the fruit rind of pomegranate (Punica granatum) antagonize in vitro the host inflammatory response mechanisms involved in the onset of malaria.

https://arctichealth.org/en/permalink/ahliterature142063
Source
Malar J. 2010;9:208
Publication Type
Article
Date
2010
Author
Mario Dell'agli
Germana V Galli
Michela Bulgari
Nicoletta Basilico
Sergio Romeo
Deepak Bhattacharya
Donatella Taramelli
Enrica Bosisio
Author Affiliation
Dipartimento di Scienze Farmacologiche, Università degli Studi di Milano, Via Balzaretti, 9 - 20133 Milano, Italy. mario.dellagli@unimi.it
Source
Malar J. 2010;9:208
Date
2010
Language
English
Publication Type
Article
Keywords
Antimalarials - pharmacology
Biological Assay
Ellagic Acid - pharmacology
Fruit
Gene Expression Regulation - drug effects
Hemeproteins - analysis
Humans
Hydrolyzable Tannins - pharmacology
Inflammation - drug therapy
Malaria, Cerebral - drug therapy
Matrix Metalloproteinase 9 - drug effects - metabolism - secretion
Matrix Metalloproteinase Inhibitors
NF-kappa B - drug effects - physiology
Phytotherapy
Plant Extracts - chemistry - pharmacology
Punicaceae - chemistry
RNA, Messenger - analysis - drug effects
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha - physiology
Up-Regulation
Abstract
The sun-dried rind of the immature fruit of pomegranate (Punica granatum) is presently used as a herbal formulation (OMARIA, Orissa Malaria Research Indigenous Attempt) in Orissa, India, for the therapy and prophylaxis of malaria. The pathogenesis of cerebral malaria, a complication of the infection by Plasmodium falciparum, is an inflammatory cytokine-driven disease associated to an up-regulation and activity of metalloproteinase-9 and to the increase of TNF production. The in vitro anti-plasmodial activity of Punica granatum (Pg) was recently described. The aim of the present study was to explore whether the anti-malarial effect of OMARIA could also be sustained via other mechanisms among those associated to the host immune response.
From the methanolic extract of the fruit rind, a fraction enriched in tannins (Pg-FET) was prepared. MMP-9 secretion and expression were evaluated in THP-1 cells stimulated with haemozoin or TNF. The assays were conducted in the presence of the Pg-FET and its chemical constituents ellagic acid and punicalagin. The effect of urolithins, the ellagitannin metabolites formed by human intestinal microflora, was also investigated.
Pg-FET and its constituents inhibited the secretion of MMP-9 induced by haemozoin or TNF. The effect occurred at transcriptional level since MMP-9 mRNA levels were lower in the presence of the tested compounds. Urolithins as well inhibited MMP-9 secretion and expression. Pg-FET and pure compounds also inhibited MMP-9 promoter activity and NF-kB-driven transcription.
The beneficial effect of the fruit rind of Punica granatum for the treatment of malarial disease may be attributed to the anti-parasitic activity and the inhibition of the pro-inflammatory mechanisms involved in the onset of cerebral malaria.
Notes
Cites: Anal Biochem. 1976 May 7;72:248-54942051
Cites: Science. 1976 Aug 20;193(4254):673-5781840
Cites: Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):325-91988933
Cites: FEBS Lett. 1998 Sep 11;435(1):29-349755853
Cites: Int J Cancer. 2005 Jan 20;113(3):423-3315455341
Cites: J Immunol. 2005 Jan 1;174(1):475-8415611273
Cites: Photochem Photobiol. 2005 Jan-Feb;81(1):38-4515493960
Cites: J Nutr. 2005 Sep;135(9):2096-10216140882
Cites: J Immunol. 2005 Nov 15;175(10):6436-4216272296
Cites: Cell Mol Life Sci. 2005 Dec;62(23):2896-90316314917
Cites: J Ethnopharmacol. 2006 Feb 20;103(3):311-816221534
Cites: J Agric Food Chem. 2006 Feb 8;54(3):980-516448212
Cites: Lab Invest. 2006 Sep;86(9):873-8816865090
Cites: J Nutr. 2006 Oct;136(10):2481-516988113
Cites: Malar J. 2006;5:8517029647
Cites: J Agric Food Chem. 2006 Nov 15;54(23):8956-6117090147
Cites: Altern Med Rev. 2008 Jun;13(2):128-4418590349
Cites: J Med Food. 2008 Jun;11(2):390-418598186
Cites: Malar J. 2008;7:15718710562
Cites: Antimicrob Agents Chemother. 2009 Feb;53(2):622-3019015351
Cites: Cancer Res. 2009 Feb 15;69(4):1502-819208844
Cites: Antimicrob Agents Chemother. 2009 Mar;53(3):1100-619015354
Cites: J Ethnopharmacol. 2009 Sep 7;125(2):279-8519577622
Cites: J Agric Food Chem. 2010 Feb 24;58(4):2246-5220102205
Cites: J Nutr Biochem. 2010 Aug;21(8):717-2519616930
Cites: Am J Chin Med. 1999;27(3-4):371-610592846
Cites: J Nat Prod. 2001 May;64(5):603-711374952
Cites: J Ethnopharmacol. 2001 Nov;78(1):85-711585693
Cites: Cell Mol Life Sci. 2003 Jul;60(7):1440-812943230
Cites: Cell Mol Life Sci. 2003 Aug;60(8):1623-3514504653
Cites: J Immunol. 2003 Oct 15;171(8):4243-5314530348
Cites: Parassitologia. 2003 Dec;45(3-4):135-4015267101
Cites: Infect Immun. 2004 Aug;72(8):4868-7315271950
Cites: Eur J Nutr. 2004 Aug;43(4):205-2015309440
Cites: Clin Chim Acta. 2004 Oct;348(1-2):63-815369737
Cites: J Immunol Methods. 1986 May 22;89(2):271-73486233
PubMed ID
20642847 View in PubMed
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