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[A method for differential cytolysis in the molecular genetic expert identification of material evidence: problems in optimizing the procedure].

https://arctichealth.org/en/permalink/ahliterature213534
Source
Sud Med Ekspert. 1996 Jan-Mar;39(1):16-21
Publication Type
Article
Author
L I Gyské
P L Ivanov
Source
Sud Med Ekspert. 1996 Jan-Mar;39(1):16-21
Language
Russian
Publication Type
Article
Keywords
Cell Fractionation - methods
DNA - analysis - isolation & purification
Electrophoresis, Agar Gel
Expert Testimony - methods - standards
Forensic Medicine - methods - standards - statistics & numerical data
Humans
Male
Polymerase Chain Reaction - methods - standards - statistics & numerical data
Regression Analysis
Russia
Spermatozoa - chemistry - ultrastructure
Abstract
The authors discuss the problem of selective derivation of the genetic material of spermatozoa for molecular genetic identification from mixed biological traces containing sperm on material evidence. Possible methods of improving the efficacy of differential lysis of cells present in mixed traces are analyzed. Effects of some routinely used extractants on biological substrata, most often contaminating the sperm in expert material, have been studied, and conditions for their most complete elimination from objects of investigation optimized.
PubMed ID
8669057 View in PubMed
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