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[Distribution of HLA specificity frequencies in patients with cystic echinococcosis].

https://arctichealth.org/en/permalink/ahliterature127303
Source
Med Parazitol (Mosk). 2011 Oct-Dec;(4):14-6
Publication Type
Article
Author
G I Lukmanova
A A Gumerov
Z M Elicheva
L I Lukmanova
Source
Med Parazitol (Mosk). 2011 Oct-Dec;(4):14-6
Language
Russian
Publication Type
Article
Keywords
Alleles
Animals
Antigenic Variation - genetics - immunology
Bashkiria
Case-Control Studies
Child
Child, Preschool
Cysts
Echinococcosis - blood - genetics - immunology
Echinococcus - immunology
Epitopes - genetics - immunology
Female
Gene Frequency
Genetic Predisposition to Disease
HLA-DQ beta-Chains - blood - genetics - immunology
HLA-DRB1 Chains - blood - genetics - immunology
Haplotypes
Heterozygote
Humans
Immunophenotyping
Male
Polymerase Chain Reaction
Suppuration
Abstract
This investigation was undertaken to study the associations of the polymorphic variants of the HLA-DRBI and HLA-DQB1 loci with the development of cystic echinococcosis in children. The material for the investigation was collected from 57 children admitted for surgery to the clinic of the Department of Pediatric Surgery, Orthopedics, and Anesthesiology, Bashkir State Medical University (Ufa). The PROTRANS kit (Germany) was used to isolate DNA samples from peripheral venous blood served as an object of the investigations. HLA specificities were typed by polymerase chain reaction. Molecular genetic studies established the association of DRB1*07, DQB1*0.9, DQB1*02 specificities with the increased risk of cystic echinococcosis in children. The echinococcosis cyst suppuration-complicated course of the disease was found to be more frequently encountered in DQB1*02 and DRB1*03 allele carriers.
PubMed ID
22308705 View in PubMed
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[Genomic typing of the Echinococcus granulosus isolates from the areas of Southern Urals].

https://arctichealth.org/en/permalink/ahliterature166268
Source
Parazitologiia. 2006 Sep-Oct;40(5):479-84
Publication Type
Article
Author
G I Lukmanova
A A Gumeov
M M Tuigunov
T V Viktorova
Source
Parazitologiia. 2006 Sep-Oct;40(5):479-84
Language
Russian
Publication Type
Article
Keywords
Adolescent
Animals
Cattle
Child
DNA, Helminth - genetics
Echinococcosis - epidemiology - genetics
Echinococcus granulosus - genetics
Female
Genotype
Humans
Male
Russia
Abstract
Nine larvocysts of Echinococcus granulosus isolated from nine patients and one cyst derived from a naturally infested cattle have been examined. Genomic typing was carried out in order to identify strains of E. granulosus. All DNA samples were shown to have the same genotype, E. granulosus G1.
PubMed ID
17144408 View in PubMed
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[Identification of the causative agent of echinococcosis in the population of the South Urals].

https://arctichealth.org/en/permalink/ahliterature158789
Source
Med Parazitol (Mosk). 2007 Oct-Dec;(4):29-31
Publication Type
Article
Author
G I Lukmanova
A A Gumerov
M A Nartailakov
F S Bilalov
A Kh Baimiev
Source
Med Parazitol (Mosk). 2007 Oct-Dec;(4):29-31
Language
Russian
Publication Type
Article
Keywords
Adolescent
Adult
Animals
Child
Child, Preschool
DNA, Helminth - genetics
DNA, Mitochondrial - genetics
Echinococcosis - parasitology
Echinococcus granulosus - anatomy & histology - classification - genetics
Electron Transport Complex IV - genetics
Humans
Larva - anatomy & histology - classification - genetics
Polymerase Chain Reaction
Russia
Species Specificity
Abstract
The topicality of the problem associated with echinococcosis granulosus in the South Urals is determined by its wide spread and a considerable economic damage made to this region by this invasion. The study was undertaken to reveal the intraspecific affiliation of Echinococcus granulosus that induces hydatid disease in the population of the South Urals. Samples for studies were taken from the fertile larval cysts obtained during intraoperative intervention in patients with hydatid disease. As morphological criteria for differentiation, the authors examined the proboscis uncuses of protoscolexes. For E. granulosus genomic typing, polymerase chain reaction (PCR) of DNA synthesis was used, as described by Gasser (1998). As a DNA marker, the authors used a fragment of the mitochondrial gene encoding for the first subunit of cytochome-C-oxidase. The DNA fragments obtained by PCR from 9 isolated underwent the direct enzyme dideoxy-sequencing test (Senger, 1977). As a result, the causative agent of echinococcosis granulosis was first identified in the patient of the South Urals. In children and adults, the clinical form of the disease is caused by E. granulosus with the genotype G - common, that of domestic sheep. Comparative analysis of molecular data revealed the presence of genotype G1 variations circulating in the South Urals homologous to the sequences recorded in the GenBank under numbers U50464 and DQ109036.
PubMed ID
18274150 View in PubMed
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[ON PREVENTION OF CYSTIC ECHINOCOCCOSIS IN THE REPUBLIC OF BASHKORTOSTAN].

https://arctichealth.org/en/permalink/ahliterature266201
Source
Gig Sanit. 2015 May-Jun;94(3):15-7
Publication Type
Article
Author
M I Lukmanov
M A Nartailakov
T R Zul'karnaev
S R Gabdrakhimov
G I Lukmanova
Source
Gig Sanit. 2015 May-Jun;94(3):15-7
Language
Russian
Publication Type
Article
Keywords
Animals
Bashkiria - epidemiology
Echinococcosis - epidemiology - prevention & control
Health Education - methods
Humans
Hygiene
Morbidity - trends
Abstract
According to official statistics, in the Republic of Bashkortostan (RB) since 1991 there is noted the increase in the number of patients with cystic echinococcosis. In 2012, the number of recorded cases increased by 55.3% if compared with 2007. In the areas of RB, where there was observed the high index of population morbidity rate, there was noted the extensive growth of the infestation in sheep. The analysis of the landscape-climatic and sanitary characteristics of the territory of the RB, as well as population economic activity revealed a definite relationship between them and the spread of echinococcosis. The performed survey of rural residents of RB showed that the population is poorly informed about the factors of transmission and ways of infection by echinococcosis. Out of respondents 73% were wrong in determining factors of transmission of infection, 34% admitted irregularities in the disposal of slaughtering condemned products. Fully correct answers were given only by 9% of respondents. The research of fruitfulness of hydatid bubbles and viability of Protoscolexes revealed that the effect of hot water (t = 90-100 ° C) is effective for disinfecting of invasive origins of larvocysts. Due to the low level of awareness of the population there is needed an active health education work on effective measures to prevent the spread of echinococcosis in RB--the prevention of feeding dogs with raw slaughtering waste and decontamination in the private courtyard slaughtering condemned products with the hot water.
PubMed ID
26302551 View in PubMed
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