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32 records – page 1 of 4.

[Analysis of the Moscow population of Neisseria meningitidis strains by the method of multilocus sequencing-typing].

https://arctichealth.org/en/permalink/ahliterature168921
Source
Zh Mikrobiol Epidemiol Immunobiol. 2006 Mar-Apr;(2):31-6
Publication Type
Article
Author
K O Mironov
A E Platonov
I S Koroleva
G A Shipulin
Source
Zh Mikrobiol Epidemiol Immunobiol. 2006 Mar-Apr;(2):31-6
Language
Russian
Publication Type
Article
Keywords
Cerebrospinal Fluid - microbiology
DNA, Bacterial - genetics
Humans
Meningococcal Infections - microbiology - prevention & control
Molecular Epidemiology
Moscow - epidemiology
Neisseria meningitidis - classification - genetics
Sequence Analysis, DNA
Serotyping
Species Specificity
Abstract
The analysis of meningococcal strains of different serogroups, isolated from the liquor of patients in Moscow, which was carried out with the method of multilocus sequencing-typing (MLST), was presented. At the periods of epidemic morbidity rises in Moscow the prevalence of group A meningococcal strains, belonging to subgroups III with sequence-types 5 (in the 1970s) and 7 (in 1996), was noted, and at a period between epidemics strains of genetic subgroups VI and X were isolated. Meningococcal strains, groups B and C, isolated in 1995 - 2002, had, as a rule, unique sequence-types, differing both one from another and from N. meningitidis sequence-types detected in other countries. Among group B meningococci the prevalence of strains belonging to clonal complex ST-18 was noted, while for group C meningococcci strains belonging to clonal complex ST-41/44 were most typical. Such genetic variability of circulating meningococci was regarded as characteristic of the period between epidemics, observed in Moscow since the end of the 1980s.
PubMed ID
16758895 View in PubMed
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[Application of molecular genetic methods during Legionnaires' disease outbreak in town Verkhnyaya Pyshma].

https://arctichealth.org/en/permalink/ahliterature157286
Source
Zh Mikrobiol Epidemiol Immunobiol. 2008 Mar-Apr;(2):23-9
Publication Type
Article
Author
S B Iatsyshina
T S Astakhova
V V Romanenko
A E Platonov
Iu V Zhukova
S I Braslavskaia
I S Tartakovskii
G A Shipulin
Source
Zh Mikrobiol Epidemiol Immunobiol. 2008 Mar-Apr;(2):23-9
Language
Russian
Publication Type
Article
Keywords
Alleles
DNA Primers
DNA, Bacterial - analysis - genetics
Disease Outbreaks
Humans
Legionella pneumophila - classification - genetics
Legionnaires' Disease - diagnosis - epidemiology
Polymerase Chain Reaction - methods
Russia
Sequence Analysis, DNA
Water Microbiology
Water Supply - analysis
Abstract
The aim of the study was to perform molecular genetic analysis based on multi-locus sequence typing in order to identify source of Legionnaires' disease outbreak in town Verkhnyaya Pyshma in July 2007 and genetic profile of the causative agent. Sequence-based typing protocol recommended by European Working Group on Legionella infection (EWGLI) was used. It was not possible to obtain satisfactory results of Fla gene sequencing for all samples. Obtained allelic profiles of other genes were typical for L. pneumophila. Allelic profiles of L. pneumophila isolated from patients were identical and matched with L. pneumophila DNA detected in water from hot water supply of domestic building, but differed from cooling tower's isolates and isolates from showerhead in apartment of one patient. Identity of 5 genes of L. pneumophila isolated from autopsy samples and from hot water of central hot water supply of domestic building confirms aspiration route of infection through hot water contaminated by the microorganism. L. pneumophila detected in water from cooling tower, showerhead in apartment of one patient, and from drainage canal of hot water supply station belonged to other allelic variants and, therefore, are not related with the outbreak.
PubMed ID
18464536 View in PubMed
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[Astroviral infection in Russian Federation].

https://arctichealth.org/en/permalink/ahliterature107453
Source
Vopr Virusol. 2013 May-Jun;58(3):32-7
Publication Type
Article
Author
A T Podkolzin
T A Konovalova
M L Iakovenko
S I Braslavskaia
G A Shipulin
Source
Vopr Virusol. 2013 May-Jun;58(3):32-7
Language
Russian
Publication Type
Article
Keywords
Adolescent
Astroviridae Infections - complications - epidemiology - virology
Child
Child, Preschool
Feces - virology
Gastroenteritis - complications - epidemiology - virology
Humans
Incidence
Infant
Infant, Newborn
Mamastrovirus - classification - genetics - isolation & purification
Phylogeny
RNA, Viral - classification - genetics
Retrospective Studies
Russia - epidemiology
Serotyping
Abstract
The astroviral infections are considered among the most common pathogens of gastroenteritis in children. The incidence, molecular epidemiology and clinical manifestations of the astrovirus infection in children hospitalized with acute gastroenteritis, in various areas of the Russian Federation from 2004 to 2010 was determined.
PubMed ID
24006631 View in PubMed
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The burden of tick-borne diseases in the Altai region of Russia.

https://arctichealth.org/en/permalink/ahliterature290986
Source
Ticks Tick Borne Dis. 2017 08; 8(5):787-794
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Date
08-2017
Author
V G Dedkov
E G Simonova
O V Beshlebova
M V Safonova
O A Stukolova
E V Verigina
G V Savinov
I P Karaseva
E A Blinova
V M Granitov
I V Arsenjeva
G A Shipulin
Author Affiliation
Central Research Institute for Epidemiology (CRIE), Federal Service on Consumer Rights Protection and Human Well-Being Surveillance, Moscow, Russia; Research Institute of Occupational Health, Moscow, Russia. Electronic address: vgdedkov@yandex.ru.
Source
Ticks Tick Borne Dis. 2017 08; 8(5):787-794
Date
08-2017
Language
English
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Keywords
Coinfection - epidemiology - microbiology - parasitology
Humans
Incidence
Lyme Disease - epidemiology - microbiology
Prevalence
Risk
Siberia - epidemiology
Spotted Fever Group Rickettsiosis - epidemiology - microbiology
Tick-Borne Diseases - epidemiology - microbiology - parasitology
Abstract
This article presents the results of a comprehensive survey of the burden of tick-borne infectious diseases (TBIDs) in the Altai region of Russia. Official data for TBID incidence were analyzed and 201 samples from patients with suspected TBID were studied. Furthermore, questing ticks and ticks recovered from humans were examined to estimate prevalence of TBID-causative agents. The Altai region was determined to have a heightened risk for TBIDs in Russia. The most epidemiologically significant tick-borne illness in this area is spotted fever group rickettsiosis, while nationally in Russia, the leading TBID is Lyme borreliosis. The prevalence of mixed infection was 12.4% among the studied cases. Additionally, the prevalence of poorly studied pathogens - Kemerovo virus (KEMV) and Rickettsia tarasevichiae - in ticks from the Altai region was determined.
PubMed ID
28648773 View in PubMed
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[Detection of sapoviruses in children with acute gastroenteritis in Moscow in 2002-2003].

https://arctichealth.org/en/permalink/ahliterature174898
Source
Vopr Virusol. 2005 Mar-Apr;50(2):27-31
Publication Type
Article
Author
A T Podkolzin
A A Mukhina
G A Shipulin
V N Kuz'mina
S I Braslavskaia
V V Maleev
A V Gorelov
N V Belova
A G Bokovoi
N B Tanina
A A Novokshenov
N V Sokolova
L N Mazankova
S V Shishkina
G Iu Iakovleva
Source
Vopr Virusol. 2005 Mar-Apr;50(2):27-31
Language
Russian
Publication Type
Article
Keywords
Adolescent
Caliciviridae Infections - diagnosis - virology
Child
Child, Preschool
Feces - virology
Female
Gastroenteritis - virology
Hospitals, Urban
Humans
Infant
Male
Molecular Sequence Data
Moscow
Phylogeny
Sapovirus - genetics - isolation & purification
Abstract
Sapoviruses were found, for the first time, to be circulating in children with acute gastroenteritis in the city of Moscow. On the basis of a genetic analysis, they were classified as belonging to genotypes 1 and 2. Two groups of sapoviruses that are essentially different from the strains presented now at the GenBank NCBI were described within the case study.
PubMed ID
15881394 View in PubMed
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Development and evaluation of a real-time RT-PCR assay for the detection of Ebola virus (Zaire) during an Ebola outbreak in Guinea in 2014-2015.

https://arctichealth.org/en/permalink/ahliterature277330
Source
J Virol Methods. 2016 Feb;228:26-30
Publication Type
Article
Date
Feb-2016
Author
V G Dedkov
N' F Magassouba
M V Safonova
A A Deviatkin
A S Dolgova
O V Pyankov
A A Sergeev
D V Utkin
G N Odinokov
V A Safronov
A P Agafonov
V V Maleev
G A Shipulin
Source
J Virol Methods. 2016 Feb;228:26-30
Date
Feb-2016
Language
English
Publication Type
Article
Keywords
Africa, Western - epidemiology
Democratic Republic of the Congo
Disease Outbreaks - prevention & control
Ebolavirus - genetics - isolation & purification
Guinea - epidemiology
Hemorrhagic Fever, Ebola - diagnosis - epidemiology
Humans
RNA, Viral - genetics
Real-Time Polymerase Chain Reaction - methods
Reverse Transcriptase Polymerase Chain Reaction - methods
Russia
Sensitivity and specificity
Abstract
In early February 2014, an outbreak of the Ebola virus disease caused by Zaire ebolavirus (EBOV) occurred in Guinea; cases were also recorded in other West African countries with a combined population of approximately 25 million. A rapid, sensitive and inexpensive method for detecting EBOV is needed to effectively control such outbreak. Here, we report a real-time reverse-transcription PCR assay for Z. ebolavirus detection used by the Specialized Anti-epidemic Team of the Russian Federation during the Ebola virus disease prevention mission in the Republic of Guinea. The analytical sensitivity of the assay is 5 × 10(2) viral particles per ml, and high specificity is demonstrated using representative sampling of viral, bacterial and human nucleic acids. This assay can be applied successfully for detecting the West African strains of Z. ebolavirus as well as on strains isolated in the Democratic Republic of the Congo in 2014.
PubMed ID
26597659 View in PubMed
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Development and evaluation of the RT-PCR kit for the rabies virus diagnosis.

https://arctichealth.org/en/permalink/ahliterature289567
Source
Vopr Virusol. 2016; 61(5):235-40
Publication Type
Journal Article
Author
Vladimir G Dedkov
A A Deviatkin
E M Poleschuk
M V Safonova
M L Markelov
G A Shipulin
Source
Vopr Virusol. 2016; 61(5):235-40
Language
English
Publication Type
Journal Article
Keywords
Animals
Cats
DNA Primers - chemical synthesis - genetics
Deer - virology
Dogs
Foxes - virology
Humans
RNA, Viral - genetics
Rabies - diagnosis - epidemiology - transmission - veterinary
Rabies virus - genetics - isolation & purification
Real-Time Polymerase Chain Reaction - methods - standards
Reverse Transcriptase Polymerase Chain Reaction - methods - standards
Russia - epidemiology
Sensitivity and specificity
Abstract
To improve the diagnosis, surveillance, and control for the rabies virus, a kit for hybridization-triggered fluorescence detection of rabies virus DNA by the RT-PCR technique was developed and evaluated. The analytical sensitivity of the kit was 4*10 GE per ml. High specificity of the kit was shown using representative sampling of viral, bacterial, and human nucleic acids.
PubMed ID
29323857 View in PubMed
Less detail

Development and evaluation of the RT-PCR kit for the rabies virus diagnosis.

https://arctichealth.org/en/permalink/ahliterature289725
Source
Vopr Virusol. 2016; 61(5):235-40
Publication Type
Journal Article
Author
Vladimir G Dedkov
A A Deviatkin
E M Poleschuk
M V Safonova
M L Markelov
G A Shipulin
Source
Vopr Virusol. 2016; 61(5):235-40
Language
English
Publication Type
Journal Article
Keywords
Animals
Cats
DNA Primers - chemical synthesis - genetics
Deer - virology
Dogs
Foxes - virology
Humans
RNA, Viral - genetics
Rabies - diagnosis - epidemiology - transmission - veterinary
Rabies virus - genetics - isolation & purification
Real-Time Polymerase Chain Reaction - methods - standards
Reverse Transcriptase Polymerase Chain Reaction - methods - standards
Russia - epidemiology
Sensitivity and specificity
Abstract
To improve the diagnosis, surveillance, and control for the rabies virus, a kit for hybridization-triggered fluorescence detection of rabies virus DNA by the RT-PCR technique was developed and evaluated. The analytical sensitivity of the kit was 4*10 GE per ml. High specificity of the kit was shown using representative sampling of viral, bacterial, and human nucleic acids.
PubMed ID
29323857 View in PubMed
Less detail

[Development and use of the assay for Legionella pneumophila detection based on fluorescent real-time/endpoint polymerase-chain reaction].

https://arctichealth.org/en/permalink/ahliterature157285
Source
Zh Mikrobiol Epidemiol Immunobiol. 2008 Mar-Apr;(2):29-37
Publication Type
Article
Author
S B Iatsyshina
S A Portenko
T S Astakhova
N A Osina
S A Rachina
S B Garanina
Iu V Zhukova
A V Shishova
T Iu Kondrat'eva
N S Cherviakova
T V Valova
N V Ivanchik
O I Krechikova
A L Durasova
A N Kulichenko
V V Romanenko
I S Tartakovskii
V V Maleev
G A Shipulin
Source
Zh Mikrobiol Epidemiol Immunobiol. 2008 Mar-Apr;(2):29-37
Language
Russian
Publication Type
Article
Keywords
Bronchoalveolar Lavage Fluid - microbiology
Colony Count, Microbial
DNA, Bacterial - analysis - genetics
Diagnosis, Differential
Fluorescence
Humans
Legionella pneumophila - genetics - isolation & purification
Legionnaires' Disease - diagnosis - epidemiology - microbiology
Lung - microbiology
Polymerase Chain Reaction - methods
Russia - epidemiology
Sensitivity and specificity
Sputum - microbiology
Water Microbiology
Water Supply - analysis
Abstract
The aim of the work was to develop a PCR-based assay for detection of L. pneumophila and L. micdadei in environmental samples as well as in clinical samples from low respiratory tract and to assess its analytic characteristics. The assay was used during investigation of the outbreak developed in July 2007 in town Verkhnyaya Pyshma (Sverdlovsk region). Polymerase-chain reaction (PCR)with fluorescent detection,sequencing and cloning of DNA fragments were used. Developed assay based on the PCR with fluorescent real-time/ endpointdetection is able to detect L. pneumophila in clinical and environmental samples and to quantify amount of bacterial DNA in water. Specificity of analysis (100%) was assessed using the panel of bacterial strains and samples from healthy individuals. Analytic sensitivity of assay and quantitation limit was 1000 GU in 1 ml. Sensitivity of the assay of artificially contaminated biological samples was 1000 bacteria in 1 ml. During outbreak investigation L. pneumophila DNAwas detected in 4 lung samples from 4 fatal cases, from 1 of 2 sputum samples, 1 of 2 bronchoalveolar lavage samples with X-ray confirmed pneumonia. Legionella's DNA was found in samples from cooling towers, central hot water supply as well as from showerheads in apartments of 3 patients. Fountain and drinking water samples were PCR-negative. Specificity of PCR-positive results was confirmed by sequencing. Use of the assay during outbreak in- vestigation allowed to confirm the diagnosis in fatal cases and quickly identify the possible source of infection.
PubMed ID
18464537 View in PubMed
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[Genetic characteristic of Haemophilus influenzae type B strains isolated in Russian regions].

https://arctichealth.org/en/permalink/ahliterature144965
Source
Zh Mikrobiol Epidemiol Immunobiol. 2010 Jan-Feb;(1):24-8
Publication Type
Article
Author
K O Mironov
A E Platonov
M K Nikolaev
I S Koroleva
G A Shipulin
Source
Zh Mikrobiol Epidemiol Immunobiol. 2010 Jan-Feb;(1):24-8
Language
Russian
Publication Type
Article
Keywords
Bacterial Typing Techniques
Genes, Bacterial - genetics
Haemophilus Infections - epidemiology
Haemophilus influenzae type b - classification - genetics
Humans
Molecular Epidemiology
Russia - epidemiology
Sequence Analysis
Abstract
Genotyping of Hib strains isolated in regions of Russia as well as characterization of genetic relations of typed strains with strains isolated in other areas.
Genetic characterization of 31 strains of Hib isolated in Russian regions during 2005-2008 was performed by multilocus sequence typing.
Studied strains belonged to 11 variants of sequence types, 6 of which were described in previous studies, whereas other 5 were isolated for the first time during this study. The most common isolated strains were ST-92 (13 strains or 42%) and ST-6 (6 strains or 19%). Typed strains were distributed to two clonal complexes. Clonal complex "A1/A2" ("ST-6") incorporates all typed strains except ST-93 strain belonging to clonal complex "B1b" ("ST-93"). The majority of studied strains (19 or 61%) had difference from "central" sequence type of clonal complex, A1/A2 ("ST-6") on not more than one allele.
Clonal structure of isolated strains is analogous to the one observed in Moscow and foreign strains.
PubMed ID
20218340 View in PubMed
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32 records – page 1 of 4.