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Analysis of Neisseria gonorrhoeae in Ontario, Canada, with decreased susceptibility to quinolones by pulsed-field gel electrophoresis, auxotyping, serotyping and plasmid content.

https://arctichealth.org/en/permalink/ahliterature208613
Source
J Med Microbiol. 1997 May;46(5):383-90
Publication Type
Article
Date
May-1997
Author
N. Harnett
S. Brown
G. Riley
R. Terro
C. Krishnan
M. Pauzé
K H Yeung
Author Affiliation
Central Public Health Laboratory, Ontario Ministry of Health, Toronto, Canada.
Source
J Med Microbiol. 1997 May;46(5):383-90
Date
May-1997
Language
English
Publication Type
Article
Keywords
4-Quinolones
Anti-Infective Agents - pharmacology
Bacterial Typing Techniques
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field
Gonorrhea - epidemiology - microbiology
Humans
Incidence
Microbial Sensitivity Tests
Neisseria gonorrhoeae - classification - drug effects
Ontario - epidemiology
Plasmids
Restriction Mapping
Serotyping
Abstract
The incidence of Neisseria gonorrhoeae with reduced susceptibility to quinolones increased from 0.18% (63 of 3285) in 1992 to 0.56% (15 of 2663) in 1993 and 0.62% (46 of 2846) in 1994. In all, 65 of the 67 isolates of Neisseria gonorrhoeae with decreased susceptibility to quinolones were characterised by pulsed-field gel electrophoresis (PFGE), auxotyping, serotyping and plasmid content. The strains were distributed among 14 auxotype/serovar (A/S) classes. Thirty isolates (46.2%) which were penicillin-susceptible with ciprofloxacin MIC90 of 0.12 mg/L and norfloxacin MIC90 of 1.0 mg/L belonged to a single A/S class, OUHL/IA-2. All but two of the 30 isolates had identical PFGE restriction profiles with NheI restriction endonuclease. Fifteen isolates (23.1%) with MICs in the intermediate (or resistant) categories for penicillin and with ciprofloxacin and norfloxacin MIC90 of 0.25 and 4.0 mg/L and (0.5 and 4.0 mg/L) respectively, belonged to A/S class P/IB-1. The 15 isolates showed nine different patterns with NheI and eight patterns with SpeI restriction endonucleases. Two of three beta-lactamase-producing (PPNG) isolates belonged to A/S class P/IB-5 and had a dissimilar PFGE restriction profile with NheI endonuclease; the other isolate belonged to A/S class P/IB-8. The remaining 17 isolates were distributed among 11 A/S classes. Three isolates within the common A/S class NR/IB-1 were subdivided into two types by PFGE as were three isolates belonging to A/S class NR/IB-2. Overall the 65 isolates of N. gonorrhoeae were distributed into 30 NheI and 26 SpeI macrorestriction profiles. All but one isolate harboured the 2.6-MDa cryptic plasmid and 18 isolates carried the 24.5-MDa transferable plasmid. The three PPNG isolates carried the 4.5-MDa Asian beta-lactamase-producing plasmid and a 25.2-MDa conjugative plasmid was found in the two TRNG isolates.
PubMed ID
9152033 View in PubMed
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Antimicrobial resistance of Salmonella isolates from human and animal sources in Ontario.

https://arctichealth.org/en/permalink/ahliterature229031
Source
Can Dis Wkly Rep. 1990 May 26;16(21):99-102
Publication Type
Article
Date
May-26-1990
Author
A H Chagla
A. Borczyk
C A Fleming
C. Krishnan
Author Affiliation
Clinical Bacteriology Section, Ontario Ministry of Health, Toronto.
Source
Can Dis Wkly Rep. 1990 May 26;16(21):99-102
Date
May-26-1990
Language
English
French
Publication Type
Article
Keywords
Animals
Disease Reservoirs
Drug Resistance, Microbial
Humans
Ontario
Salmonella - drug effects - isolation & purification
PubMed ID
2357739 View in PubMed
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Congenital tuberculosis in a twin of immigrant parentage.

https://arctichealth.org/en/permalink/ahliterature248079
Source
Can Med Assoc J. 1978 Nov 4;119(9):1068-70
Publication Type
Article
Date
Nov-4-1978
Author
M R Dische
C. Krishnan
R. Andreychuk
M W Fujiwara
Source
Can Med Assoc J. 1978 Nov 4;119(9):1068-70
Date
Nov-4-1978
Language
English
Publication Type
Article
Keywords
Diseases in Twins
Emigration and Immigration
Female
Hepatic Veins
Humans
Infant
Iran - ethnology
Male
Ontario
Phlebitis - congenital - genetics
Pregnancy
Pregnancy Complications, Infectious
Tuberculosis - congenital - genetics
Tuberculosis, Cardiovascular - congenital - genetics
Tuberculosis, Hepatic - congenital - genetics
Tuberculosis, Lymph Node - congenital - genetics
Tuberculosis, Pulmonary - congenital - genetics - transmission
Notes
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Cites: Arch Dis Child. 1952 Apr;27(132):167-7214924686
Cites: Can Med Assoc J. 1966 Feb 26;94(9):420-520328514
PubMed ID
311240 View in PubMed
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Decreased susceptibility of Neisseria gonorrhoeae to fluoroquinolones--Ontario, 1992-1994.

https://arctichealth.org/en/permalink/ahliterature215796
Source
Can Commun Dis Rep. 1995 Feb 15;21(3):17-20; discussion 20-1
Publication Type
Article
Date
Feb-15-1995
Author
N. Harnett
S. Brown
G. Riley
R. Terro
C. Krishnan
Author Affiliation
STD Laboratory, Ontario Ministry of Health, Toronto.
Source
Can Commun Dis Rep. 1995 Feb 15;21(3):17-20; discussion 20-1
Date
Feb-15-1995
Language
English
French
Publication Type
Article
Keywords
Anti-Infective Agents - pharmacology
Drug Resistance, Microbial
Female
Fluoroquinolones
Gonorrhea - epidemiology - microbiology
Humans
Male
Neisseria gonorrhoeae - drug effects
Ontario - epidemiology
PubMed ID
7881379 View in PubMed
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Infection due to Yersinia enterocolitica in a series of patients with beta-thalassemia: incidence and predisposing factors.

https://arctichealth.org/en/permalink/ahliterature203533
Source
Clin Infect Dis. 1998 Dec;27(6):1362-6
Publication Type
Article
Date
Dec-1998
Author
T V Adamkiewicz
M. Berkovitch
C. Krishnan
C. Polsinelli
D. Kermack
N F Olivieri
Author Affiliation
Hospital for Sick Children, Toronto, Canada.
Source
Clin Infect Dis. 1998 Dec;27(6):1362-6
Date
Dec-1998
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Canada - epidemiology
Causality
Chelating Agents - therapeutic use
Child
Child, Preschool
Cohort Studies
Deferoxamine - therapeutic use
Female
Humans
Incidence
Male
Retrospective Studies
Yersinia Infections - drug therapy - epidemiology - etiology
Yersinia enterocolitica - isolation & purification
beta-Thalassemia - complications - microbiology
Abstract
Over 15 years, 14 patients with yersiniosis in two North American comprehensive thalassemia clinics (0.6 cases per 100 patient-years) presented with fever (100%), diarrhea (86%), right-lower-quadrant abdominal pain (71%), bacteremia (57%), a palpable abdominal mass (36%), and pharyngitis (28%). Clinically apparent infection occurred within 10 days of blood transfusion in 57% of patients. Nine patients (64%) had only a modest elevation in serum level of ferritin (
Notes
Comment In: Clin Infect Dis. 1998 Dec;27(6):1367-89868643
PubMed ID
9868642 View in PubMed
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Isolates of Neisseria gonorrhoeae in Ontario showing chromosomally-mediated resistance to penicillin combined with plasmid-mediated resistance to tetracycline.

https://arctichealth.org/en/permalink/ahliterature214550
Source
Can Commun Dis Rep. 1995 Aug 15;21(15):139-43
Publication Type
Article
Date
Aug-15-1995

Laboratory investigation of outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7.

https://arctichealth.org/en/permalink/ahliterature235149
Source
J Clin Microbiol. 1987 Jun;25(6):1043-7
Publication Type
Article
Date
Jun-1987
Author
C. Krishnan
V A Fitzgerald
S J Dakin
R J Behme
Source
J Clin Microbiol. 1987 Jun;25(6):1043-7
Date
Jun-1987
Language
English
Publication Type
Article
Keywords
Aged
Aged, 80 and over
Animals
Bacterial Toxins - analysis - biosynthesis
Colitis - epidemiology
Culture Media
Cytotoxins - analysis - biosynthesis
Disease Outbreaks
Escherichia coli - classification - metabolism
Escherichia coli Infections - epidemiology
Feces - chemistry - microbiology
Gastrointestinal Hemorrhage - epidemiology - etiology
Glucuronidase - metabolism
Homes for the Aged
Humans
Nursing Homes
Ontario
Shiga Toxin 1
Vero Cells
Abstract
A severe outbreak of hemorrhagic colitis occurred in London, Ontario, during the month of September 1985. A total of 55 residents and 18 employees of a nursing home developed diarrhea, and 17 residents (age range, 78 to 99 years) died. Specimens from 38 patients, 37 employees and contacts, and 10 autopsies were investigated for all enteric pathogens. Specimens were also planted on MacConkey-sorbitol agar. Fecal extracts were tested on Vero cells for cytotoxin (FVT). Escherichia coli isolates were serotyped and tested for verotoxin and beta-glucuronidase production. Of the 38 symptomatic patients, 26 were positive for FVT, verotoxin-producing E. coli (VTEC), or both. Of the 105 specimens that were examined from these 38 patients, FVT and VTEC were both positive in 30 specimens, FVT only was positive in 13 specimens, and VTEC only was positive in 4 specimens. None of the 27 specimens from 10 autopsies was positive for FVT or VTEC. No other enteric pathogen was found in any of the cases. All asymptomatic individuals were negative for both FVT and VTEC. Of 19 VTEC strains that were isolated, 18 belonged to serotype O157:H7. These 18 strains and 2 more strains that were obtained from sporadic cases that had occurred within the 2 previous months were found to give similar biochemical reactions in a 36-test identification system. All isolates of serotype O157:H7 were beta-glucuronidase negative and susceptible to the antimicrobial agents that are used to treat E. coli infections. Testing for FVT and VTEC was found to be the most sensitive and specific technique for the laboratory diagnosis of this disease. Negative sorbitol, positive raffinose, and negative beta-glucuronidase tests appeared to be consistent markers for aiding in the detection of E. coli O157:H7.
Notes
Cites: J Infect Dis. 1985 May;151(5):775-823886804
Cites: J Clin Microbiol. 1985 Jan;21(1):46-763881471
Cites: J Clin Microbiol. 1985 Oct;22(4):614-93908473
Cites: J Clin Microbiol. 1985 Oct;22(4):620-53908474
Cites: J Clin Microbiol. 1986 May;23(5):869-723519658
Cites: J Infect Dis. 1986 Oct;154(4):631-83528316
Cites: J Clin Pathol. 1952 Nov;5(4):361-213011228
Cites: Antimicrob Agents Chemother. 1976 Oct;10(4):598-603988781
Cites: Infect Immun. 1977 Dec;18(3):775-9338490
Cites: Appl Environ Microbiol. 1982 Jun;43(6):1320-97049088
Cites: Lancet. 1983 Jan 1;1(8314-5):766129412
Cites: Lancet. 1983 Mar 19;1(8325):619-206131302
Cites: N Engl J Med. 1983 Mar 24;308(12):681-56338386
Cites: J Clin Microbiol. 1983 Sep;18(3):512-206355145
Cites: J Clin Microbiol. 1984 Feb;19(2):172-46365956
Cites: Ann Intern Med. 1984 Dec;101(6):738-426388450
Cites: Appl Environ Microbiol. 1984 Oct;48(4):855-66391379
Cites: J Infect Dis. 1985 Oct;152(4):775-73900234
PubMed ID
3298310 View in PubMed
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Molecular characterization of multiresistant strains of Salmonella typhi from South Asia isolated in Ontario, Canada.

https://arctichealth.org/en/permalink/ahliterature205073
Source
Can J Microbiol. 1998 Apr;44(4):356-63
Publication Type
Article
Date
Apr-1998
Author
N. Harnett
S. McLeod
Y. AuYong
J. Wan
S. Alexander
R. Khakhria
C. Krishnan
Author Affiliation
Clinical Bacteriology Section, Central Public Health Laboratory, Toronto, ON, Canada.
Source
Can J Microbiol. 1998 Apr;44(4):356-63
Date
Apr-1998
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Anti-Bacterial Agents - pharmacology
Asia - epidemiology
Bacterial Typing Techniques
Canada - epidemiology
Child
Child, Preschool
Citric Acid - metabolism
Conjugation, Genetic
DNA Fingerprinting
Drug Resistance, Microbial - genetics
Drug Resistance, Multiple - genetics
Electrophoresis, Gel, Pulsed-Field
Humans
Infant
Mercury - pharmacology
Microbial Sensitivity Tests
Middle Aged
Plasmids - drug effects
Salmonella paratyphi A - classification - isolation & purification
Salmonella typhi - classification - drug effects - isolation & purification
Travel
Typhoid Fever - epidemiology - microbiology
Abstract
Two hundred and fourteen isolates of Salmonella typhi submitted to our laboratory between 1992 and 1996 were tested for susceptibility to 20 antimicrobial agents. Forty-eight of the 214 isolates (22.4%), recovered from individuals who had travelled in South Asia, were multiresistant. Forty-four of the 48 isolates were resistant to ampicillin, chloramphenicol, tetracycline, streptomycin, sulfamethoxazole, trimethoprim, cotrimoxazole, ticarcillin, and piperacillin; the other four isolates were resistant to four to six agents. Forty-two of the multiresistant isolates belonged to Vi phage type E1, two isolates from the Punjab State belonged to phage type A, another from the Punjab State belonged to phage type E3, one isolate from Pakistan belonged to type M1, and one isolate from India belonged to type J1. Plasmids from 45 of 48 isolates showed a temperature-sensitive mechanism of transfer to Escherichia coli K-12 strains, characteristic of H1 incompatibility group plasmids. The majority of plasmids had an estimated molecular weight of 120 MDa and encoded both citrate utilization and mercury resistance. Plasmids from three isolates had an estimated molecular weight of 112-115 MDa; one of these isolates encoded citrate utilization but not mercury resistance. Analysis of isolates by pulsed-field gel electrophoresis after digestion with XbaI and SpeI indicated that the majority of multiresistant isolates shared a common restriction profile, while four isolates had unique patterns.
PubMed ID
9674108 View in PubMed
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13 records – page 1 of 2.